The Versatility Of HiSorb-GC×GC-qMS To Explore Brewed Coffee Volatiles

Solid phase microextraction (SPME) as a well-known high concentration capacity (HCC) is more and more used in combination with comprehensive two-dimensional gas chromatography to investigate the food-related volatiles. A more recent HCC tool with a much higher extractive phase named HiSorb¿ was released in the past few years. This higher sorbent volume drastically increases the sensitivity compared to the SPME, as does the stir bar sorptive extraction (SBSE). However, SBSE requires additional manipulation which considerably limit its automation and may lead to lower repeatability. The HiSorb¿ combine the advantages of both SPME and SBSE, respectively, the easy handling and automation and the higher sensitivity. In this project, the versatility of the novel HiSorb¿ was explored through the investigation of brewed coffee volatiles. The HiSorb¿ were coupled with comprehensive two-dimensional gas chromatography quadrupole mass spectrometry (GC×GC-qMS) equipped with a flow modulator to enhance the separation and maximise the level of information extractable from the obtained chromatographic fingerprint. In addition, the use of multi-cumulative extraction provided more informative-rich data which highly benefits from the use of modern chemometrics tools providing more comprehensive information.