[en] The aim of this study was to assess the adverse effects of enrofloxacin (EF) on Tra catfish, Pangasianodon hypophthalmus, in relation with density stress. Fish were held at 40, 80 or 120 fish m(-3) and fed with pellets containing either 1 g kg(-1) EF or no EF. Antibiotic exposure lasted 7d and all fish were fed without EF for another 7-d recovery period. Fish were sampled at 3, 7, 8, 10 and 14 d after the beginning of EF exposure. Lipid peroxidation (LPO) and total glutathione (GSH) levels, catalase (CAT), glutathione-s-transferase (GST) and acetylcholine-esterase (AChE) activities were assessed in gill, brain, liver and muscle. At day 7, LPO levels in gills of EF-fish reared at low or high density were significantly more than 5-fold higher than their respective control. On the contrary, LPO in gills of EF-fish reared at medium density was significantly 3-fold lower than the control fish. Similarly, CAT activities in gills of EF-fish reared under low or high density were higher than in their control groups, while this activity was lower in EF-fish of the medium density group. AChE activities in muscles of EF-fish reared at low or high density were lower than controls at days 3 and 7, respectively. These results suggest that EF exposure may lead to disorders like lipid peroxidation and neural dysfunction in fish. However, when reared under lower stress condition (medium density), they may cope better with EF-induced stress than chronically stressed fish (low or high density).
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Animal production & animal husbandry
Author, co-author :
Wang, Neil; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP
Nkejabega, Noemie; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP
Hien, Nguyen-Ngoc
Huynh, Thi-Tu
Silvestre, Frederic; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP
Phuong, Nguyen-Thanh
Danyi, Sophie ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Widart, Joëlle ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Douny, Caroline ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Kestemont, Patrick; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP
Huong, Do-Thi-Thanh
Language :
English
Title :
Adverse effects of enrofloxacin when associated with environmental stress in Tra catfish (Pangasianodon hypophthalmus).
Ahmad I., Fatima M., Athar M., Khan N.Z., and Raisuddin S. Responses of circulating fish phagocytes to paper milt effluent exposure. Bull. Environ. Contam. Toxicol. 61 (1998) 746-753
Ahmad I., Pacheco M., and Santos M.A. Enzymatic and nonenzymatic antioxidants as an adaptation to phagocyte-induced damage in Anguilla anguilla L. following in situ habor water exposure. Ecotoxicol. Environ. Saf. 57 (2004) 290-302
Baudhuin P., Baufay H., Rahman-Li Y., Sellinger O.Z., Wattiaux R., Jacques P., and De Duve C. Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, d-amino acid oxidase and catalase in rat-liver tissue. Biochem. J. 92 (1964) 179-184
Christ W. Central nervous system toxicity of quinolones: human and animal findings. J. Antimicrob. Chemother. 26 (1990) 219-225
Della Rocca G., Di Salvo A., Malvisia J., and Sello M. The disposition of enrofloxacin in sea bream (Sparus aurata L.) after single intravenous injection or from medicated feed administration. Aquaculture 232 (2004) 53-62
Ellman G.L., Courtney K.O., Anders V., and Featherstone R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7 (1961) 88-95
Fatima M., Ahmad I., Sayeed I., Athar M., and Raisuddin S. Pollutant-induced over-activation of phagocytes is concomitantly associated with peroxidative damage in fish tissues. Aquat. Toxicol. 49 (2000) 243-250
Gurbay A., Gonthier B., Daveloose D., Favier A., and Hincal F. Microsomal metabolism of ciprofloxacin generates free radicals. Free Radical Biol. Med. 30 (2001) 1118-1121
Gurbay A., Gonthier B., Signorini-Allibe N., Barret L., Favier A., and Hincal F. Ciprofloxacin-induced DNA damage in primary culture of rat astrocytes and protection by vitamin E. Neurotoxicology 27 (2006) 6-10
Gurbay A., Gonthier B., Barret L., Favier A., and Hincal F. Cytotoxic effect of ciprofloxacin in primary culture of rat astrocytes and protection by vitamin E. Toxicology 229 (2007) 54-61
Habig W.H., Pabst M.J., and Jakoby W.B. Glutathione S-transferases. The first step in mercapturic acid formation. J. Biol. Chem. 249 (1974) 7120-7130
Head A.B., and Malison J.A. Effects of lighting spectrum and disturbance level on the growth and stress responses of Yellow perch, Perca flavescens. J. World Aquacult. Soc. 31 (2000) 73-80
Hecht T., and Uys W. Effect of density on the feeding and aggressive behaviour in juvenile African catfish, Clarias gariepinus. S. Afr. J. Sci. 87 (1997) 537-541
Intorre L., Cecchini S., Bertini S., Cognetti Varriale A.M., Soldani G., and Mengozzi G. Pharmacokinetics of enrofloxacin in the seabass (Dicentrarchus labrax). Aquaculture 182 (2000) 49-59
Kim M.S., Lim J.H., Park B.K., Hwang Y.H., and Yun H.I. Pharmacokinetics of enrofloxacin in Korean catfish (Silurus asotus). J. Vet. Pharmacol. Ther. 29 (2006) 397-402
Littell R.C., Milliken G.A., Stroup W.W., and Wolfinger R.D. SAS® System for Mixed Models (1996), SAS Institute Inc., Cary, NC 633 pp
Lowry O.H., Rosenbruogh N.J., Tarr A.I., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
Matos P., Fontainhas-Fernandes A., Peixoto F., Carrola J., and Rocha E. Biochemical and histological hepatic changes of Nile tilapia Oreochromis niloticus exposed to carbaryl. Pestic. Biochem. Physiol. 89 (2007) 73-80
Minta M., Wilk I., and Zmudzki J. Inhibition of cell differentiation by quinolones in micromass cultures of rat embryonic limb bud and midbrain cells. Toxicol. in Vitro 19 (2005) 915-919
Montero D., Marrero M., Izquierdo M.S., Robaina L., Vergara J.M., and Tort L. Effect of vitamin E and C dietary supplementation on some immune parameters of gilthead seabream, Sparus aurata, juveniles subjected to crowding stress. Aquaculture 171 (1999) 269-278
Oikari A. Caging techniques for field exposures of fish to chemical contaminants. Aquat. Toxicol. 78 (2006) 370-381
Ozcan Oruc E., and Usta D. Evaluation of oxidative stress responses and neurotoxicity potential of diazinon in different tissues of Cyprinus carpio. Environ. Toxicol. Pharmacol. 23 (2007) 48-55
Payne J.F., Mathieu A., Melvin W., and Fancey L.L. Acetylcholinesterase, an old biomarker with a new future? Field trials in association with two urban rivers and a paper mill in Newfoundland. Mar. Pollut. Bull. 32 (1996) 225-231
Rotllant J., Pavlidis M., Kentouri M., Abad M.E., and Tort L. Non-specific immune responses in the red porgy Pagrus pagrus after crowding stress. Aquaculture 156 (1997) 279-290
Rotllant J., Tort L., Montero D., Pavlidis M., Martinez M., Wendelaar-Bonga S.E., and Balm P.H.M. Background colour influence on the stress response in cultured red porgy Pagrus pagrus. Aquaculture 223 (2003) 129-139
Song S.B., Xu Y., and Zhou B.S. Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio). Chemosphere 65 (2006) 699-706
Sprandel K.A., and Rodvold K.A. Safety and tolerability of fluoroquinolones. Clin. Cornerstone 3 (2003) 29-35
Stahlmann R. Clinical toxicological aspects of fluoroquinolones. Toxicol. Lett. 127 (2002) 269-277
Stoffregen D.A., Wooster G.A., Bustos P.S., Bowser P.R., and Babish J.G. Multiple route and dose pharmacokinetics of enrofloxacin in juvenile Atlantic salmon. J. Vet. Pharmacol. Ther. 20 (1997) 111-123
Sun Y., Hongxia Y., Zhang J., Yin Y., Shi H., and Wang X. Bioaccumulation, depuration and oxidative stress in fish Carassius auratus under phenanthrene exposure. Chemosphere 63 (2006) 1319-1327
Toussaint B., Bordin G., Janosi A., and Rodriguez A.R. Validation of a liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 11 (fluoro)quinolone antibiotics in swine kidney. J. Chromatogr. 976 (2002) 195-206
Toussaint B., Chedin M., Bordin G., and Rodriguez A.R. Determination of (fluoro) quinolone antibiotic residues in pig kidney using liquid chromatography-tandem mass spectrometry I. Laboratory-validated method. J. Chromatogr. 1088 (2005) 32-39
Vaccaro E., Giorgi M., Longo V., Mengozzi G., and Gervasi P.G. Inhibition of cytochrome P450 enzymes by enrofloxacin in the sea bass (Dicentrarchus labrax). Aquat. Toxicol. 62 (2003) 27-33
Wang N., Gardeur J.N., Henrotte E., Marie M., Kestemont P., and Fontaine P. Determinism of the induction of the reproductive cycle in Eurasian perch, Perca fluviatilis: identification of environmental cues and permissive factors. Aquaculture 261 (2006) 706-714
Yin Z., Lam T.J., and Sin Y.M. The effect of crowding stress on the non specific immune response of fancy carp (Cyprinus carpio L.). Fish Shellfish Immunol. 5 (1995) 519-529
Yin Y., Jia H., Sun Y., Yu H., Wang X., Wu J., and Xue Y. Bioaccumulation and ROS generation in liver of Carassius auratus, exposed to phenantrene. Comp. Biochem. Physiol. 145 (2007) 288-293
Zhang L.R., Li M.H., Cheng N.N., Chen B.Y., and Wang Y.M. Inhibition by fluoroquinolones of K+ currents in rat dissociated hippocampal neurons. Eur. J. Pharmacol. 462 (2003) 9-13