Article (Scientific journals)
Effect of ppMCH derived peptides on PBMC proliferation and cytokine expression
Coumans, Bernard; Grisar, Thierry; Nahon, J. L. et al.
2007In Regulatory Peptides, 143 (1-3), p. 104-108
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Keywords :
prepro-MCH (ppMCH); melanin concentrating hon-none (MCH); neuropeptide gluramic acid-isoleucine (NEI); neuropeptide glycine-glutamic acid (NGE); peptide MCH-NEl; peripheral blood mononuclear cell (PBMC); cytokines
Abstract :
[en] The mRNA encoding prepro-Melanin concentrating hormone (ppMCH) is mainly expressed in the central nervous system but has also been detected at lower amount in many peripheral tissues including spleen and thymus. At the peptide level however, several forms of the precursor can be detected in these tissues and are sometimes expressed at similar levels compared to brain. In the present work, we have studied the in vitro action of a wide range of concentration (1 nM to 1 microM) of the different peptides encoded by ppMCH i.e. neuropeptide glycine-glutamic acid (NGE), neuropeptide glutamic acid-isoleucine (NEI), Melanin concentrating hormone (MCH) and the dipeptide NEI-MCH on peripheral blood mononuclear cells (PBMC) proliferation and cytokine production following anti-CD3 stimulation. Among them only MCH decreased PBMC proliferation with a maximal effect of 35% at 100 nM. Moreover as demonstrated by using ELISA, MCH significantly decreases IL-2 production by 25% but not IL-4, INF-gamma or TNF-alpha expression. Interestingly, exogenous IL-2 decreases significantly MCH-mediated inhibition, suggesting that it is an important downstream mediator of MCH action. Finally, we showed that after 7 to 9 days of incubation, MCH also inhibits proliferation of non-stimulated PBMC. Altogether, these data demonstrate that fully mature MCH modulates proliferation of anti-CD3 stimulated PBMC partially through regulation of IL-2 production.
Disciplines :
Endocrinology, metabolism & nutrition
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Coumans, Bernard ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Grisar, Thierry ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Nahon, J. L.
Lakaye, Bernard ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Language :
English
Title :
Effect of ppMCH derived peptides on PBMC proliferation and cytokine expression
Publication date :
04 October 2007
Journal title :
Regulatory Peptides
ISSN :
0167-0115
eISSN :
1873-1686
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
143
Issue :
1-3
Pages :
104-108
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 November 2009

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