Human immune cells express ppMCH mRNA and functional MCHR1 receptor

[en] Melanin-concentrating hormone (MCH) is highly expressed in the brain and modulates feeding behavior. It is also expressed in some peripheral tissues where its role remains unknown. We have investigated MCH function in human and mouse immune cells. RT-PCR analysis revealed a low expression of prepro-MCH and MCH receptor 1 (MCHR1) but not of MCHR2 transcript in tissular and peripheral blood immune cells. FACS and in vitro assay studies demonstrated that MCHR1 receptor expression on most cell types can trigger, in the presence of MCH, cAMP synthesis and calcium mobilization in peripheral blood mononuclear cells (PBMCs). Moreover, MCH treatment decreases the CD3-stimulated PBMC proliferation in vitro. Accordingly, our data indicate for the first time that MCH and MCHR1 may exert immunomodulatory functions. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Verlaet, Myriam ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine)

Adamantidis, Antoine ; Université de Liège - ULiège > Biochimie et physiologie humaine et pathologique

Coumans, Bernard ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.

Chanas, G.

Zorzi, Willy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

Grisar, Thierry ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique

Lakaye, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique

Language :

English

Title :

Human immune cells express ppMCH mRNA and functional MCHR1 receptor

Publication date :

11 September 2002

Journal title :

FEBS Letters

ISSN :

0014-5793

eISSN :

1873-3468

Publisher :

Elsevier Science Bv, Amsterdam, Netherlands

Volume :

527

Issue :

1-3

Pages :

205-210

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 October 2009

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