[en] Magnistipida butayei subsp. montana (Chrysobalanaceae) is known, in the Great Lakes Region, to possess toxicological properties. In this paper, we investigated the acute toxicity (dose levels 50-1600 mg/kg) of its aqueous extract, administered orally to adult Wistar rats. This study demonstrated that the freeze-dried aqueous extract (5%, w/w) possesses high toxicity. The extract caused hypothermia, neurological disorders, including extensor reflex of maximal convulsive induced-seizures at about 2h after the administered dose, and death occurred (LD50 = 370 mg/kg) in a dose dependent manner. Blood parameter evaluation revealed slight variations, but these might not have clinical relevance. Histological examination of internal organs (lungs, liver, heart and kidneys) did not reveal any abnormality in the treated group compared to the control. Therefore, it can be concluded that Magnistipida butayei subsp. montana aqueous extract, given orally, is toxic and that its target is the central nervous system. General phytochemical screening revealed that the plant did not contain significant amounts of products known to be toxic, such as alkaloids or cardioactive glycosides, but only catechic tannins, amino acids, saponins and other aphrogen principles in the three parts of the species (fruit, leave and bark). (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Research center :
Centre Interfacultaire de Recherche du Médicament (C.I.R.M.)
Kadima, Justin N.; Université Nationale du Rwanda - UNR > Pharmacie
Damas, Jacques ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Noirfalise, Alfred ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Language :
English
Title :
Chemical and biological investigations of a toxic plant from Central Africa, Magnistipula butayei subsp montana
S. Ahlenius, M. Oprica, C. Eriksson, B. Winblad, and M. Schultzberg Effects of kainic acid on rat body temperature: unmasking by dizocilpine Neuropharmacology 43 2002 28 35
Angenot, L., 1970. Essais Phytochimiques Préliminaires sur Quelques Plantes Médicinales du Rwanda Oriental Plantes Médicinales et Phytothérapie, Tome IV, no4, pp. 263-278.
J. Astrup, G. Blenow, and B. Nilsson Effects of reduced cerebral blood flow upon EEG pattern, cerebral extracellular potassium and energy metabolism in the rat cortex during bicuculline-induced seizures Brain Research 9 1979 115 126
D. Banauch, W. Brummer, W. Ebeling, H. Metz, H. Rindfrey, H. Lang, K. Leybold, and W. Rick A glucose dehydrogenase for the determination of glucose concentrations in body fluids Zeitschnift für Klinische Chemie und Klinische Biochemie 13 1975 101 107
B.E. Bastidas Ramirez, N. Navarro Ruiz, J.D. Quezada Arellano, B. Ruiz Madrigal, M.T. Villanueva Michel, and P. Garzon Anticonvulsant effects of Magnolia grandiflora L. in the rat Journal of Ethnopharmacology 61 1998 143 152
S. Desouter Pharmacopée humaine et vétérinaire du Rwanda 1991 IRST Butare, Rwanda p. 104
B.P. Devi, R. Boominathan, and S.C. Mandal Evaluation of antipyretic potential of Cleome viscosa L. (Capparidaceae) extract in rats Journal of Ethnopharmacology 87 2003 11 13
W.C. Evans Trease and Evans' Pharmacognosy 14th ed. 1996 W.B. Saunders Company Ltd
L.S. Goodman, and A. Gilman The Pharmacological Basis of Therapeutics ninth ed. 1996 MacGraw-Hill, Professions Division New York p. 267
R.G. Hogan, K.L. Pickering, R.P. Dodge, J.B. Shepard, and S. Master Incidence of seizures that follow rehydration of hypernatremic rabbits with intravenous glucose or fructose solutions Experimental Neurology 87 1985 249 259
International Committee for Standardization in Haematology, 1978. Recommendations for reference method for haemoglobinometry in human blood (ICSH standard EP 6/2: 1977) and specifications for international haemoglobincyanide reference preparation (ICSH standard EP 6/3: 1977). Journal of Clinical Pathology 31, 139-143.
D.D. Johnson, R. Wilcox, J.M. Tuchek, and R.D. Crawford Experimental febrile convulsions in epileptic chickens: the anticonvulsant effect of elevated r-aminobutyric acid concentrations Epilepsia 26 1985 466 471
D. Kanjanapothi, A. Panthong, N. LertPrasertsuke, T. Taesotikul, C. Rujjanawate, D. Kaewpinit, R. Rudthayakorn, W. Choochote, U. Chaitong, A. Jitpakdi, and B. Pitasawat Toxicity of crude rhizome extract of Kaempferia galanga L. (Proh Hom) Journal of Ethnopharmacology 90 2004 359 365
Karangwa, C., 2002. Contribution à l'étude chimique et toxicologique de Magnistipula butayei De Wild. (Brenam) ssp montana (Hauman) F. White. Mémoire de DEA (Diplôme d'Etudes Approfondies) en Sciences Pharmaceutiques, Université de Liège, Belgium, p. 18.
M.A. Lennox-Buchtal Febrile convulsions O. Magnus Lorentz de Hass The Epilepsies 1974 North-Holland Amsterdam 246 263
T. Maeda, K. Hashizume, and T. Tanaka Effect of hypothermia on kainic acid-induced limbic seizures: an electroencephalographic and 14C-deoxyglucose autradiographic study Brain Research 818 1999 228 235
E.H. Malone, and R.C. Robichaud A Hippocratic screen for pure or crude drugs materials Journal of Natural Products 25 1962 320 332
J.A. McCaughram, and N. Schetcher Experimental febrile convulsions: long-term effects of hyperthermia-induced convulsions in the developing rat Epilepsia 27 1982 173 183
S. Meites Skin puncture and blood collecting techniques for infants: update and problems S. Meites Pediatric Clinical Chemistry third ed. 1989 American Association for Clinical Chemistry Washington, DC 5 15
M.E. Meyerhoff, and W.N. Opdyke Ion-selective electrodes H.E. Spiel Advances in Clinical Chemistry vol. 25 1986 Academic Press Inc. New York
T. Morimoto, M. Fukuda, Y. Aibara, H. Nagao, and K. Kida The influence of blood gas changes on hyperthermia-induced seizures in developing rats Developmental Brain Research 92 1996 77 80
S. Mraovitch, and Y. Calando Interactions between limbic, thalamo-striatal-cortical, and central autonomic pathways during epileptic seizure progression The Journal of Comparative Neurology 411 1999 145 161
K.B. Nelson, and J.H. Ellenberg Prenatal and perinatal antecedents of febrile seizures Annals of Neurology 27 1990 127 131
Potron, G., Culioli-Pickel, B. Behar, C., Droullé, C., Nguyen, P., Adjizian, J.C., 1990. Automation en hématologie. Editions techniques. Encyclopédie Médicale et Chirurgicale. Paris, France, p. 19.
A.J. Quick The prothrombin in haemophilia and in obstructive jaundice Journal of Biological Chemistry 109 1935 73 74
C.C. Sanchez-Mateo, B. Prado, and R.M. Rabanal Antidepressant effects of the methanol extract of several Hypericum species from the Canary Islands Journal of Ethnopharmacology 79 2002 119 127
Staner, P., Boutique, R., 1937. Matériaux pour l'étude des plantes médicinales indigènes du Congo Belge. Mémoires, Institut Royal Colonial Belge, Sections des Sciences Naturelles et Médicales, Bruxelles, Librairie Falk fils, G. Van Campenhout, Tome V, vol. 8, p. 48.
Y. Takei, Y. Nishikawa, M. Tachibana, T. Takami, T. Miyajima, A. Hoshika, and S. Takashima Hypothermia during kainic acid-induced seizures reduces hippocampal lesions and cerebral nitric oxide production in immature rabbits Brain and Development 26 2004 176 183
V. Tancredi, R. D'Arcangelo, C. Zona, A. Siniscalchi, and M. Avoli Induction of epileptiform activity by temperature elevation in hippocampal slices from young rats: an in vitro model for febrile seizures Epilepsia 33 1992 228 234
S.F. Traynelis, and R. Dingledine Potassium-induced spontaneous electrographic seizures in the rat hippocampal slice Journal of Neurophysiology 59 1988 259 276
Troupin, G., 1978. Flore du Rwanda, Spermatophytes I, XIV, p. 413.
E.E. Türk, F. Schulz, E. Koops, A. Gehl, and M. Tsokos Fatal hypernatremia after using salt as an emetic - report of three autopsy cases Legal Medicine 7 2005 47 50
H. Wagner, and S. Bladt Plants Drug Analysis: A Thin Layer Chromatography Atlas second ed. 1996 Spring pp. 306-364
Y. Yager, A.E. Armstrong, C. Jaharus, M.D. Saucier, and C.E. Wirrell Preventing hyperthermia decreases brain damage following neonatal hypoxic-ischemic seizures Brain Research 1011 2004 48 57