Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers

Myopathie atypique; Érable; Prévention; Acer; Acer pseudoplatanus; Pâture; Atypical myopathy; Maple; Prevention; Pasture; Ruminants; Toxine; Toxin; Intoxication; Poisoning; Microbiote; Microbiota

Abstract :

[en] Equine atypical myopathy (AM) is caused by hypoglycin A (HGA) and methylenecyclopropylglycine (MCPG) intoxication resulting from the ingestion of seeds or seedlings of some Acer tree species. Interestingly, not all horses pasturing in the same toxic environment develop signs of the disease. In other species, it has been shown that the intestinal microbiota has an impact on digestion, metabolism, immune stimulation and protection from disease. The objective of this study was to characterize and compare fecal microbiota of horses suffering from AM and healthy co-grazers. Furthermore, potential differences in fecal microbiota regarding the outcome of diseased animals were assessed. This prospective observational study included 59 horses with AM (29 survivors and 30 nonsurvivors) referred to three Belgian equine hospitals and 26 clinically healthy co-grazers simultaneously sharing contaminated pastures during spring and autumn outbreak periods. Fresh fecal samples (rectal or within 30min of defecation) were obtained from all horses and bacterial taxonomy profiling obtained by 16S amplicon sequencing was used to identify differentially distributed bacterial taxa between AM-affected horses and healthy co-grazers. Fecal microbial diversity and evenness were significantly (p < 0.001) higher in AM-affected horses as compared with their non-affected co-grazers. The relative abundance of families Ruminococcaceae, Christensenellaceae and Akkermansiaceae were higher (p ≤ 0.001) whereas those of the Lachnospiraceae (p = 0.0053), Bacteroidales (p < 0.0001) and Clostridiales (p = 0.0402) were lower in horses with AM, especially in those with a poor prognosis. While significant shifts were observed, it is still unclear whether they result from the disease or might be involved in the onset of disease pathogenesis.

Research Center/Unit :

FARAH - Fundamental and Applied Research for Animals and Health - ULiège

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Wimmer-Scherr, Christina ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés

Taminiau, Bernard ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Renaud, Benoît ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)

van loon, Gunther

Palmers, Kathrien

Votion, Dominique ; Université de Liège - ULiège > Scientifiques attachés au Doyen (F MV)

Amory, Hélène ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés

Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Cesarini Latorre, Carlota ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés

Language :

English

Title :

Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers

Publication date :

15 February 2021

Journal title :

Animals

ISSN :

2076-2615

Special issue title :

Equine microbiota

Volume :

Issue :

506

Peer reviewed :

Peer reviewed

Additional URL :

https://doi.org/10.3390/ani11020506

Funders :

SPW Agriculture, Ressources naturelles et Environnement - Service Public de Wallonie. Agriculture, Ressources naturelles et Environnement

Available on ORBi :

since 18 February 2021

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