Lactic acid bacteria; Fatty acids; phospholipids class separation; evaporative light-scattering detection; survival; temperature
Abstract :
[en] The total lipids of Tsukamurella paurometabola C-924 were analysed after freeze-drying. Seven individual
lipids classes were identified namly neutral lipids (NLs), fatty acids (FAs), phospholipids (PLs), sterol ester
(SEs), triglycerides (TGs), diglycerides (DGs) and monoglycerides (MGs). The principal fatty acids
identified in most lipid classes were palmitic (C16:0), palmitoleic (C16:1), oleic (C18:1), linoleic (C18:2), and
linolenic (C18:3). PLs were the major constituents and accounted for 50-60% of the total lipids. PLs were
fractionated. . PLs of Tsukamurella paurometabola content phosphatidic acid (PA), phosphatidylethanolamine
(PE), phosphatidylinositol (PI), phosphatidylcholine (PC), sphingomyelin (SM), lysophosphatidylcholine
(LPC) and phosphatidylglycerol (PG). It was observed that PG had the highest proportion at most points
relative to other PLs and was the predominant component of PLs (30%-56%). Evolution of individual rate
was followed during stored at 20°C and 40°C with or without lithothamne400®, respectively.
