Chenopodium ambrosioides induces an endothelium-dependent relaxation of rat isolated aorta

Chenopodiaceae; Vasorelaxation; Ca-activated potassium channels; Thin-layer chromatography; High-performance liquid chromatography; Plant; Complementary and alternative medicine

Abstract :

[en] Objective This study aims to evaluate the vasodilatory effect of Chenopodium ambrosioides on the isolated rat aorta, and to explore its mechanism of action. Methods The vasorelaxant effect and the mode of action of various extracts from the leaves of C. ambrosioides were evaluated on thoracic aortic rings isolated from Wistar rats. In addition, ethyl acetate and methanol fractions were analyzed, using thin-layer chromatography and high-performance liquid chromatography techniques, for their polyphenolic content. Results The various active extracts of C. ambrosioides at four concentrations (10−3, 10−2, 10−1 and 1 mg/mL) relaxed the contraction elicited by phenylephrine, in a concentration-dependent manner. This effect seems to be endothelium-dependent, since the vasodilatory effect was entirely absent in denuded aortic rings. The vasorelaxant effect of the methanol fraction (MF) of C. ambrosioides at 1 mg/mL was also inhibited by atropine and tetraethylammonium. This effect remained unchanged by Nω-nitro-l-arginine methyl ester hydrochloride and glibenclamide. The preliminary phytochemical analysis showed that the leaves of C. ambrosioides are rich in phenolic and flavonoid derivatives. Conclusion These results suggest that the MF of C. ambrosioides produces an endothelium-dependent relaxation of the isolated rat aorta, which is thought to be mediated mainly through stimulation of the muscarinic receptors, and probably involving the opening of Ca2+-activated potassium channels.

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Assaidi, Asmae

Dib, Ikram

Tits, Monique ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie

Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie

Bellahcen, Said

Bouanani, Nourelhouda

Legssyer, Abdelkhaleq

Aziz, Mohammed

Mekhfi, Hassane

Bnouham, Mohammed

Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie

Ziyyat, Abderrahim

Language :

English

Title :

Chenopodium ambrosioides induces an endothelium-dependent relaxation of rat isolated aorta

Publication date :

2019

Journal title :

Journal of Integrative Medicine

ISSN :

2095-4964

Publisher :

Elsevier, Singapore

Volume :

Issue :

Pages :

115 - 124

Peer reviewed :

Peer reviewed

Additional URL :

http://www.sciencedirect.com/science/article/pii/S2095496419300068

Available on ORBi :

since 18 March 2019

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