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Comparison between swabbing devices in order to analyse the microbial flora found on surfaces by classical microbiology and 16S rDNA amplicon sequencing
Krings, Simone; Crevecoeur, Sébastien; Rodriguez Diaz, Cristina et al.
20174th FARAH-Day
 

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Keywords :
Surfaces; Food Microbiology; Swabbing; Community kitchens
Abstract :
[en] The work in community kitchens involves several steps bearing the risk of transmitting pathogenic microorganisms from static or dynamic surfaces to the food. This study aimed at comparing the recovery efficiency of different sampling devices in terms of culture methods and culture-independent 16S rDNA amplicon sequencing for characterising the microbial flora. In order to mimic surfaces found in community kitchens, sterile stainless steel and polypropylene surfaces were spiked with a two-fold raw chicken meat dilution and several concentrations of Escherichia coli, Salmonella Enteritidis and Bacillus cereus, respectively. The ideal swab for kitchen analyses should recover the highest number of viable bacteria with the highest population diversity. Classical culture method showed that recovery was possible with all swabs, but that Sponge-Sticks had the highest recovery rates. The 16S rDNA amplicon sequencing revealed a high relative abundance of Bacillus genus in cotton pad, gauze pad and Sponge-Stick samples and low proportions of Salmonella genus in all samples. Analysis of Escherichia genus results was problematic for molecular analyses as it appeared as a source of DNA contamination of the reagents used during library creation. Furthermore, it was possible to decipher bias populations in the sponge samples (from neutralising buffer) and in controls (due to low amounts of DNA), allowing the exclusion of those in the following analyses, if needed. In the end, these results attest the similarity of population diversity in cotton pad, gauze pad and Sponge-Stick samples, leaving the final choice to the operator.
Disciplines :
Microbiology
Author, co-author :
Krings, Simone ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Crevecoeur, Sébastien ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires
Rodriguez Diaz, Cristina ;  Université de Liège - ULiège > R&D Direction : Chercheurs ULg en mobilité
Fall, Papa Abdoulaye;  Genalyse Partner S.A.
Taminiau, Bernard  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Daube, Georges  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Language :
English
Title :
Comparison between swabbing devices in order to analyse the microbial flora found on surfaces by classical microbiology and 16S rDNA amplicon sequencing
Publication date :
13 October 2017
Event name :
4th FARAH-Day
Event organizer :
FARAH - Université de Liège
Event place :
Liège, Belgium
Event date :
13th October 2017
Available on ORBi :
since 20 September 2018

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