Physico-chemical approach for characterizing probiotics at the solid and dispersed states

Probiotics; Physico-chemistry; Thermogravimetry; Differential Scanning Calorimetry; Solid particles; Dispersed particles; Hydrophobicity; Zeta potential; Hydrodynamic diameter

Abstract :

[en] A physico-chemical approach was used for characterizing and generating fingerprints of single (L. Bulgaricus and S. thermophilus) and multiple (Vivomixx) probiotic species. Such a methodology included thermal, colloidal, and surface analyses of powder (solid-in-gaseous phase) and dispersed (solid-in-aqueous phase) samples. Decomposition and transition phases analyzed by thermogravimetry and differential scanning calorimetry provide specific qualitative and quantitative data that serve as a probiotic fingerprint, and therefore a product quality control for each sample. Investigation of colloidal and surface properties of dispersed samples by light scattering and contact angle measurements informs on the probiotic size average, electrokinetic charge, and surface hydrophobicity. Besides their relevance in identity control, the physico-chemical data are also useful in probiotic performance prediction, since they govern the most crucial microbial functionalities such as thermostability, aggregation, and adhesion.

Research Center/Unit :

TERRA Teaching and Research Centre - TERRA (CARE FoodisLife)

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Razafindralambo, Hary ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > SMARTECH

Delvigne, Frank ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Blecker, Christophe ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > SMARTECH

Language :

English

Title :

Physico-chemical approach for characterizing probiotics at the solid and dispersed states

Publication date :

2019

Journal title :

Food Research International

ISSN :

0963-9969

eISSN :

1873-7145

Publisher :

Elsevier, Netherlands

Volume :

116

Pages :

897-904

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

PHYSCHEMPROBIO

Funders :

ULiège FSR - Université de Liège. Fonds spéciaux pour la recherche

Funding text :

Fonds spéciaux de l'ULiege (FSR-F-GABT-16/2)

Available on ORBi :

since 13 September 2018

Statistics

Number of views

135 (18 by ULiège)

Number of downloads

9 (6 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Alupoaei, C.E., Olivares, J.A., Garcı́a-Rubio, L.H., Quantitative spectroscopy analysis of prokaryotic cells: Vegetative cells and spores. Biosensors and Bioelectronics 19 (2004), 893–903.
Arjmandi-Tash, O., Kovalchuk, N.M., Trybala, A., Kuchin, I.V., Starov, V., Kinetics of wetting and spreading of droplets over various substrates. Langmuir 33 (2017), 4367–4385.
Basile, F., Beverly, M.B., Voorhees, K.J., Hadfield, T.L., Pathogenic bacteria: Their detection and differentiation by rapid lipid profiling with pyrolysis mass spectrometry. TrAC Trends in Analytical Chemistry 17 (1998), 95–109.
Boonaert, C., Rouxhet, P.G., Surface of lactic acid bacteria: Relationships between chemical composition and physicochemical properties. Applied and Environmental Microbioilogy 66 (2000), 2548–2554.
Dean, J.A., Analytical chemistry handbook. 1995, McGraw-Hill.
Eschlbeck, E., Bauer, S.A., Kulozik, U., Effect of cultivation pH on the surface hydrophobicity of Bacillus subtilis spores. AMB Express, 7, 2017, 157.
FAO/WHO, Report on joint FAO/WHO expert consultation on health and nutritional properties of probiotics in food including power milk with live lactic acid bacteria. 2001, FAO.
Fiorucci, S.P., Biagioli, M., Cipriani, S., Carino, A., Marchianò S., Laghi, L., Scarpelli, P., Metabolic variability of a multispecies probiotic preparation impacts on the anti-inflammatory activity. Frontiers in Pharmacology, 8, 2017, 505.
Foligné B., Daniel, C., Pot, B., Probiotics from research to market: The possibilities, risks and challenges. Current Opinion in Microbiology 16 (2013), 284–292.
Guarner, F., Khan, A.G., Garisch, J., Eliakim, R., Gangl, A., Thomson, A., Paula, J.A.d., World gastroenterology organisation global guidelines: Probiotics and prebiotics october 2011. Journal of Clinical Gastroenterology 46 (2012), 468–481.
Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein, D.J., Pot, B., Salminen, S., Expert consensus document: The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology & Hepatology 11 (2014), 506–514.
Jelesarov, I., Bosshard, H.R., Isothermal titration calorimetry and differential scanning calorimetry as complementary tools to investigate the energetics of biomolecular recognition. Journal of Molecular Recognition 12 (1999), 3–18.
Katz, A., Alimova, A., Xu, M., Rudolph, E., Shah, M.K., Savage, H.E., Alfano, R.R., Bacteria size determination by elastic light scattering. IEEE Journal of Selected Topics in Quantum Electronics 9 (2003), 277–287.
Kotzamanidis, C., Kourelis, A., Litopoulou-Tzanetaki, E., Tzanetakis, N., Yiangou, M., Evaluation of adhesion capacity, cell surface traits and immunomodulatory activity of presumptive probiotic Lactobacillus strains. International Journal of Food Microbiology 140 (2010), 154–163.
Lee, Y.K., Salminen, S., Handbook of probiotics and prebiotics. 2009, John Wiley & Sons.
Loskyll, J., Maier, W.F., Stoewe, K., Application of a simultaneous TGA-DSC thermal analysis system for high-throughput screening of catalytic activity. ACS Combinatorial Science 14 (2012), 600–604.
Low, D., Ahlgren, J.A., Horne, D., McMahon, D.J., Oberg, C.J., Broadbent, J.R., Role of Streptococcus thermophilus MR-1C capsular exopolysaccharide in cheese moisture retention. Applied and Environmental Microbiology 64 (1998), 2147–2151.
Mackey, B.M., Miles, C.A., Parsons, S.E., Seymour, D.A., Thermal denaturation of whole cells and cell components of Escherichia coli examined by differential scanning calorimetry. Microbiology 137 (1991), 2361–2374.
Mishra, A., Kavita, K., Jha, B., Characterization of extracellular polymeric substances produced by micro-algae Dunaliella salina. Carbohydrate Polymers 83 (2011), 852–857.
Nguyen, H.T., Razafindralambo, H., Blecker, C., N'Yapo, C., Thonart, P., Delvigne, F., Stochastic exposure to sub-lethal high temperature enhances exopolysaccharides (EPS) excretion and improves Bifidobacterium bifidum cell survival to freeze–drying. Biochemical Engineering Journal 88 (2014), 85–94.
Nguyen, H.-T., Truong, D.-H., Kouhoundé S., Ly, S., Razafindralambo, H., Delvigne, F., Biochemical engineering approaches for increasing viability and functionality of probiotic bacteria. International Journal of Molecular Sciences, 17, 2016, 867.
Oelschlaeger, T.A., Mechanisms of probiotic actions–a review. International Journal of Medical Microbiology 300 (2010), 57–62.
Pembrey, R.S., Marshall, K.C., Schneider, R.P., Cell surface analysis techniques: What do cell preparation protocols do to cell surface properties?. Applied and Environmental Microbiology 65 (1999), 2877–2894.
Rabetafika, H., Razafindralambo, A., Razafindralambo, H., Probiotics from food and non-food sources. Razafindralambo, H., (eds.) Trends in probiotic applications, 2018, Studium Press LCC, Houston, TX, 33–49.
Razafindralambo, H., Trends in probiotic applications. 1st ed., 2018, Studium Press LCC, Houston, TX.
Razafindralambo, H., Advances in physical chemistry tools for probiotic characterization. Hary, R., (eds.) Trends in probiotic applications, 2018, Studium Press LCC, Houston, TX, 50–81.
Sangami, R., Sri, S.R., Emerging trends in improving viability, advanced stability techniques and health claims of healthy microbiome–the probiotics. International Journal of Current Microbiology and Applied Sciences 6 (2017), 194–200.
Saxelin, M., Probiotic formulations and applications, the current probiotics market, and changes in the marketplace: A European perspective. Clinical Infectious Diseases 46 (2008), S76–S79.
Singh, R.P., Shukla, M.K., Mishra, A., Kumari, P., Reddy, C.R.K., Jha, B., Isolation and characterization of exopolysaccharides from seaweed associated bacteria Bacillus licheniformis. Carbohydrate Polymers 84 (2011), 1019–1026.
Snyder, A.P., Maswadeh, W.M., Wick, C.H., Dworzanski, J.P., Tripathi, A., Comparison of the kinetics of thermal decomposition of biological substances between thermogravimetry and a fielded pyrolysis bioaerosol detector. Thermochimica Acta 437 (2005), 87–99.
Snyder, A.P., Tripathi, A., Dworzanski, J.P., Maswadeh, W.M., Wick, C.H., Characterization of microorganisms by thermogravimetric analysis–mass spectrometry. Analytica Chimica Acta 536 (2005), 283–293.
Soccol, C.R., Vandenberghe, L.P., De, S., Spier, M.R., Medeiros, A.B.P., Yamaguishi, C.T., Thomaz-Soccol, V., The potential of probiotics: A review. Food Technology and Biotechnology 48 (2010), 413–434.
Van der Mei, H.C., Bos, R., Busscher, H.J., A reference guide to microbial cell surface hydrophobicity based on contact angles. Colloids and Surfaces B: Biointerfaces 11 (1998), 213–221.
Van Loosdrecht, M.C., Lyklema, J., Norde, W., Schraa, G., Zehnder, A.J., Electrophoretic mobility and hydrophobicity as a measured to predict the initial steps of bacterial adhesion. Applied and Environmental Microbiology 53 (1987), 1898–1901.
Wang, Q., Cen, Z., Zhao, J., The survival mechanisms of thermophiles at high temperatures: An angle of omics. Physiology 30 (2015), 97–106.
Wei, J.B., Shull, J.C., Lee, Y.-J., Hawley, M.C., Characterization of asphalt binders based on chemical and physical properties. International Journal of Polymer Analysis and Characterization 3 (1996), 33–58.