The potency of commercial blackcurrant juices to induce relaxations in porcine coronary artery rings is not correlated to their antioxidant capacity but to their anthocyanin content
Endothelial function ; Blackcurrant; Vasorelaxation; fruit juice; Polyphenols
Abstract :
[en] Objective: Polyphenol-rich products such as fruit juices have been shown to have strong antioxidant capacities and to induce potent endothelium-dependent relaxations. We have evaluated whether the commercial blackcurrant juices induced endothelium-dependent relaxation of isolated coronary arteries can be related to their antioxidant capacity and/or phenolic content.
Research Methods & Procedures: Six different commercial blackcurrant juices were selected. Their main phenolic compounds were measured by UPLC and antioxidant capacity was evaluated by spectrometric methods. Vascular reactivity studies with these juices were done using isolated porcine coronary arteries.
Results: The six different commercial blackcurrant juices induced relaxations ranging from 21 to 100 % at the concentration of 0.5% v/v. The relaxation induced at 0.5 % v/v was not correlated to their antioxidant capacity measured by either ORAC or DPPH assays, and also not to the ascorbic acid, total polyphenols, total flavonols and total phenolic acid contents. In contrast, the amplitude of the relaxation was correlated to the total anthocyanins content and the individual anthocyanin concentration.
Conclusions: Correlations between relaxation amplitude and total anthocyanin or individual anthocyanin contents are of interest for the development of functional blackcurrant beverages with the potential to promote vascular protection.
Disciplines :
Biotechnology
Author, co-author :
Tabart, Jessica ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Auger, Cyril; University of Strasbourg
Kevers, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Dommes, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Pollet, Brigitte; University of Strasbourg
Defraigne, Jean-Olivier ; Université de Liège - ULiège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Schini-Kerth, Valérie; University of Strasbourg
PINCEMAIL, Joël ; Centre Hospitalier Universitaire de Liège - CHU > Service de chirurgie cardio-vasculaire et thoracique
Language :
English
Title :
The potency of commercial blackcurrant juices to induce relaxations in porcine coronary artery rings is not correlated to their antioxidant capacity but to their anthocyanin content
Hertog, M.G.L., Feskens, E.J.M., Kromhout, D., Hollman, P.C.H., Katan, M.B., Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen Elderly Study. Lancet 342 (1993), 1007–1011.
Hertog, M.G., Kromhout, D., Aravanis, C., Blackburn, H., Buzina, R., Fidana, F., et al. Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Arch Intern Med 155 (1995), 381–386.
Yamagata, K., Tagami, M., Yamori, Y., Dietary polyphenols regulate endothelial function and prevent cardiovascular disease. Nutrition 31 (2015), 28–37.
Artero, A., Artero, A., Tarín, J.J., Cano, A., The impact of moderate wine consumption on health. Maturitas 80 (2015), 3–13.
Patel, S., Green tea as a nutraceutical: the latest developments. Food Sci Technol Res 19 (2013), 923–932.
Corti, R., Flammer, A.J., Hollenberg, N.K., Luscher, T.F., Cocoa and cardiovascular health. Circulation 119 (2009), 1433–1441.
Schini-Kerth, V.B., Étienne-Selloum, N., Chataigneau, T., Auger, C., Vascular protection by natural product-derived polyphenols: in vitro and in vivo evidence. Planta Med 77 (2011), 1161–1167.
Wallace, T.C., Slavin, M., Frankenfield, C.L., Systematic review of anthocyanins and markers of cardiovascular disease. Nutrients, 8, 2016, 32.
Mink, P.J., Srafford, C.G., Barraj, I.M., Harnack, I., Hong, C.P., Nettleton, J.A., et al. Flavonoid intake and cardiovascular disease mortality: a prospective study in postmenopausal women. Am J Clin Nutr 85 (2007), 895–909.
Cassidy, A., O'Reilly, É.J., Kay, C., Sampson, L., Franz, M., Forman, J.P., et al. Habitual intake of flavonoid subclasses and incident hypertension in adults. Am J Clin Nutr 93 (2011), 338–347.
Cassidy, A., Mukamal, K.J., Liu, L., Franz, M., Eliassen, A.H., Rimm, E.B., High anthocyanin intake is associated with a reduced risk of myocardial infarction in young and middle-aged women. Circulation 127 (2013), 188–196.
Di Castelnuovo, A., Costanzo, S., Bagnardi, V., Donati, M.B., Iacoviello, L., De Gaetano, G., Alcohol dosing and total mortality in men and women: An updated meta-analysis of 34 prospective studies. Arch Intern Med 166 (2006), 2437–2445.
Naruszewicz, M., Łaniewska, I., Millo, B., Dłuzniewski, M., Combination therapy of statin with flavonoids rich extract from chokeberry fruits enhanced reduction in cardiovascular risk markers in patients after myocardial infraction (MI). Atherosclerosis 194 (2007), 179–184.
Zhu, Y., Bo, Y., Wang, X., Lu, W., Wang, X., Han, Z., et al. The effects of anthocyanins on blood pressure. A PRISMA-compliant meta-analysis of randomized clinical trials. Medicine (Baltimore) 95 (2016), 1–7.
Zhu, Y., Xia, M., Yang, Y., Liu, F., Li, Z., Hao, Y., et al. Purified anthocyanin supplementation improves endothelial function via NO-cGMP activation in hypercholesterolemic individuals. Clin Chem 57 (2011), 1524–1533.
Kent, K., Charlton, K.E., Jenner, A., Roodenrys, S., Acute reduction in blood pressure following of anthocyanin-rich cherry juice may be dose-interval dependent: a pilot cross-over study. Int J Food Sci Nutr 671 (2016), 47–52.
Auger, C., Kim, J.-H., Trinh, S., Chataigneau, T., Popken, A.M., Schini-Kerth, V.B., Fruit juice-induced endothelium-dependent relaxations in isolated porcine coronary arteries: evaluation of different fruit juices and purees and optimization of a red fruit juice blend. Food Funct 2 (2011), 245–250.
Auger, C., Pollet, B., Arnold, C., Marx, C., Schini-Kerth, V.B., Great heterogeneity of commercial fruit juices to induce endothelium-dependent relaxations in isolated porcine coronary arteries: role of the phenolic content and composition. J Med Food 18 (2015), 128–136.
Asgary, S., Sahebkar, A., Afshani, M.R., Keshvari, M., Haghjooyjavanmard, S., Rafieian-Kopaei, M., Clinical evaluation of blood pressure lowering, endothelial function improving, hypolipidemic and anti-inflammatory effects of pomegranate juice in hypertensive subjects. Phytother Res 28 (2014), 193–199.
Kevers, C., Pincemail, J., Defraigne, J.O., Dommes, J., Antioxidant capacity of small dark fruits. Influence of cultivars and harvest time. J Berry Res 4 (2014), 97–105.
Nakamura, Y., Matsumoto, H., Todoki, K., Endothelium-dependent vasorelaxation induced by black currant concentrate in rat thoracic aorta. Jpn J Pharmacol 89 (2002), 29–35.
Jin, Y., Alimbetov, D., George, T., Gordon, M.H., Lovegrove, J.A., A randomized trial to investigate the effects of acute consumption of a blackcurrant juice drink on markers of vascular reactivity and bioavailability of anthocyanins in human subjects. Eur J Clin Nutr 65 (2011), 849–856.
Khan, F., Ray, S., Craigie, A.M., Kennedy, G., Hill, A., Barton, K.L., et al. Lowering of oxidative stress improves endothelial function in healthy subjects with habitually low intake of fruit and vegetables: A randomized controlled trial of antioxidant- and polyphenol-rich blackcurrant juice. Free Radic Biol Med 72 (2014), 232–237.
Mialache-Arion, C., Tabart, J., Kevers, C., Niculaua, M., Filimon, R., Beceanu, D., et al. Antioxidant potential of different plum cultivars during storage. Food Chem 146 (2014), 485–491.
Association of Vitamin Chemists, Methods of vitamin assay. 3rd ed, 1961, Interscience Publishers, New York.
Sipel, A., Kevers, C., Pincemail, J., Grygiel, P.G., Defraigne, J.O., Dommes, J., Sample dilution influences the determination of antioxidant capacity in food: How to minimize it?. Food Anal Methods 6 (2013), 1485–1491.
Hollands, W., Brett, G.M., Radreau, P., Saha, S., Teucher, B., Bennett, R.N., et al. Processing blackcurrants dramatically reduces the content and does not enhance the urinary yield of anthocyanins in human subjects. Food Chem 108 (2008), 869–878.
Mattila, P.H., Hellström, J., McDougall, G., Dobson, G., Pihlava, J.M., Tiirikka, T., et al. Polyphenol and vitamin C contents in European commercial blackcurrant juice products. Food Chem 127 (2011), 1216–1223.
Goodman, C.L., Fawcett, S., Barringer, S.A., Flavor, viscosity, and color analyses of hot and cold break tomato juices. J Food Sci 67 (2002), 404–408.
Khurana, S., Venkataraman, K., Hollingsworth, A., Piche, M., Tai, T.C., Polyphenols: benefits to the cardiovascular system in health and aging. Nutrients 5 (2013), 3779–3827.
Tabart, J., Kevers, C., Dardenne, N., Schini-Kerth, V., Albert, A., Dommes, J., Deriving a global antioxidant score for commercial juices by multivariate graphical and scoring techniques: applications to blackcurrant juice. Preedy, R., (eds.) Processing and impact on antioxidants in beverages, 2014, Academic Press, Oxford, UK, 301–307.
Xu, J.W., Ikeda, K., Yamori, Y., Upregulation of endothelial nitric oxide synthase by cyanidin-3-glucoside, a typical anthocyanin pigment. Hypertension 44 (2004), 217–222.
Edirisinghe, I., Banaszewski, K., Cappozzo, J., McCarthy, D., Burton-Freeman, B.M., Effect of black currant anthocyanins on the activation of endothelial nitric oxide synthase (eNOS) in vitro in human endothelial cells. J Agric Food Chem 59 (2011), 8616–8624.
Bell, D.R., Gochenaur, K., Direct vasoactive and vasoprotective properties of anthocyanin-rich extracts. J Appl Physiol 100 (2006), 1164–1170.
Andriambeloson, E., Magnier, C., Haan-Archipoff, G., Lobstein, A., Anton, R., Beretz, A., et al. Natural dietary polyphenolic compounds cause endothelium-dependent vasorelaxation in rat thoracic aorta. J Nutr 128 (1998), 2324–2333.
Milbury, P.E., Vita, J.A., Blumberg, J.B., Anthocyanins are bioavailable in humans following an accurate dose of cranberry juice. J Nutr 140 (2010), 1099–1104.
Hassellund, S.S., Flaa, A., Kjeldsen, S.E., Seljeflot, I., Karlsen, A., Erlund, I., et al. Effects of anthocyanins on cardiovascular risk factors and inflammation in pre-hypertensive men: a double-blind randomized placebo-controlled crossover study. J Human Hypertens 27 (2012), 100–106.
Jennings, A., Welch, A.A., Spector, T., Macgregor, A., Cassidy, A., Intakes of anthocyanins and flavones are associated with biomarkers of insulin resistance and inflammation in women. J Nutr 144 (2014), 202–208.
Clark, J.L., Zahrada, P., Taylor, C.G., Efficacy of flavonoids in the management of high blood pressure. Nutr Rev 73 (2015), 799–822.
Matsumoto, H., Inaba, H., Kishi, M., Tominaga, S., Hirayama, M., Tsuda, T., Orally administered delphinidin 3-rutinoside and cyanidin 3-rutinoside are directly absorbed in rats and humans and appear in the blood as the intact forms. J Agric Food Chem 49 (2001), 1546–1551.
