Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis: Investigation of immune-related parameters

Milla, S.; Massart, Sébastien; Mathieu, C.; Wang, N.; Douny, Caroline; Douxfils, J.; Scippo, Marie-Louise; De Pauw, Edwin; Dieu, M.; Silvestre, F.; Kestemont, P.

doi:10.1016/j.cbd.2017.11.005

Article (Scientific journals)

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis: Investigation of immune-related parameters

Milla, S.; Massart, Sébastien; Mathieu, C. et al.

2018 • In Comparative Biochemistry and Physiology. Part D, Genomics and Proteomics, 25, p. 86-98

Peer reviewed

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https://hdl.handle.net/2268/221087

DOI
10.1016/j.cbd.2017.11.005

PubMed
29223774

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Keywords :

Corticosteroids; Eurasian perch; Glucose metabolism; Immunity; Proteomics; Article; Perca fluviatilis

Abstract :

[en] The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80 mg/kg) or DOC (4 mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Implantation of cortisol decreased the ratio of blood leucocytes and depressed Ig levels in both organs while DOC modulated the proportion of leucocyte sub-populations (increase in lymphocytes and decrease in granulocytes). In contrast, the innate humoral immunity was not strongly influenced by any of corticosteroid implants. The only immune parameter that was significantly affected was lysozyme, after DOC treatment. A number of proteins were differentially regulated by these hormones and some were identified in the liver (21 for cortisol and 8 for DOC) and in the spleen (10 for cortisol and 10 for DOC). None of the proteins was directly linked to immunity, except the natural killer enhancing factor, which was repressed by cortisol in the spleen. Our results also confirm that the proteins involved in energetic and glucose metabolism are affected by corticosteroids. Furthermore, these corticosteroids differently regulate immune status in Eurasian perch and they primarily impact leucocytes, as opposed to innate immune function. © 2017 Elsevier Inc.

Research center :

FARAH - Fundamental and Applied Research for Animals and Health - ULiège

Disciplines :

Aquatic sciences & oceanology

Author, co-author :

Milla, S.; University of Namur, Research Unit in Environmental and Evolutionary Biology, rue de Bruxelles 61, Namur, Belgium, Université de Lorraine, Unité de Recherche Animal et Fonctionnalités des Produits Animaux, USC INRA 340, Vandoeuvre-lès-Nancy, France

Massart, Sébastien ; Université de Namur - UNamur

Mathieu, C.; University of Namur, Research Unit in Environmental and Evolutionary Biology, rue de Bruxelles 61, Namur, Belgium

Wang, N.; University of Namur, Research Unit in Environmental and Evolutionary Biology, rue de Bruxelles 61, Namur, Belgium

Douny, Caroline ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires

Douxfils, J.; University of Namur, Research Unit in Environmental and Evolutionary Biology, rue de Bruxelles 61, Namur, Belgium

Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Dieu, M.; University of Namur, Research Unit in Cellular Biology, rue de Bruxelles 61, Namur, Belgium

Silvestre, F.; University of Namur, Research Unit in Environmental and Evolutionary Biology, rue de Bruxelles 61, Namur, Belgium

Kestemont, P.; University of Namur, Research Unit in Environmental and Evolutionary Biology, rue de Bruxelles 61, Namur, Belgium

Language :

English

Title :

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis: Investigation of immune-related parameters

Publication date :

2018

Journal title :

Comparative Biochemistry and Physiology. Part D, Genomics and Proteomics

ISSN :

1744-117X

Publisher :

Elsevier Inc.

Volume :

Pages :

86-98

Peer reviewed :

Peer reviewed

Name of the research project :

2. 4570. 06

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 06 March 2018

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