[en] We developed a sensitive UHPLC-MS/MS method with a simple yet efficient sample collection and preparation protocol to accurately quantify E4 in whole blood. This method was then successfully applied for the pharmacokinetic study of E4 in a murin model providing the best administration route for E4. The innovative sampling allowed decreasing the number of animals used for the study as VAMS only draws few microliters of blood thus leaving the mice alive for the whole PK study reducing the inter-individual variability.
Research Center/Unit :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Nys, Gwenaël ; Université de Liège > Département de pharmacie > Analyse des médicaments
Servais, Anne-Catherine ; Université de Liège > Département de pharmacie > Analyse des médicaments
Pequeux, Christel ; Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Fillet, Marianne ; Université de Liège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Pharmacokinetic study of estetrol in murin model developing a whole blood microsampling, extraction procedure and an UHPLC-MS/MS method
