Reference : A surprising and dramatic neuroendocrine-immune phenotype of mice deficient in Growth...
Scientific congresses and symposiums : Poster
Human health sciences : Immunology & infectious disease
A surprising and dramatic neuroendocrine-immune phenotype of mice deficient in Growth Hormone-Releasing Hormone (GHRH)
Farhat, Khalil mailto [Université de Liège - ULiège > > > Form. doc. sc. bioméd. & pharma.]
Bodart, Gwennaëlle mailto [Université de Liège - ULiège > > > Form. doc. sc. bioméd. & pharma.]
Renard, chantal mailto []
Desmet, Christophe mailto [Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire >]
MOUTSCHEN, Michel mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service des maladies infectieuses - médecine interne >]
BARON, Frédéric mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service d'hématologie clinique >]
Beguin, Yves mailto [Université de Liège > > GIGA-R : Hématologie >]
Salvatori, Roberto [Johns Hopkins University > Endocrinology > > >]
Martens, Henri mailto [Université de Liège > > Centre d'immunologie >]
GEENEN, Vincent [Centre Hospitalier Universitaire de Liège - CHU > Centre d'immunologie > Service d'endocrinologie clinique > >]
The 19th European Congress of Endocrinology.
20/05/2017 - 23/05/2017
The European Society of Endocrinology
[en] Growth hormone ; Infectious diseases ; S.pneumoniae
[en] In the framework of close interactions between the immune and neuroendocrine systems, Growth Hormone (GH) has been proposed to exert significant effects on the immune system, but there is not yet a consensus about GH immunomodulatory properties.
These studies investigated the immune and anti-infectious response of dwarf Ghrh-/- mice presenting a severe deficiency of the GHRH/GH/IGF-1 axis.
In basal conditions, thymic parameters and T-cell responses of Ghrh-/- mice were not severely affected but a constant B-cell lymphopaenia was observed. Thus, we investigated vaccine and anti-infectious responses of Ghrh-/- mice toward Streptococcus pneumonia,
a B-dependent pathogen,
Ghrh-/- mice were unable to trigger production of specific IgM and IgG against serotype 1 pneumococcal polysaccharide (PPS) after vaccination with either native PPS (Pnx23) or protein-PPS conjugate (Prev-13) vaccines. These vaccines both include the serotype 1 (our S.pneumoniae strain) and provide an effective protection in mice. A short GH supplementation to Ghrh-/- mice (1 daily injection of 1 mg/kg GH for 4 weeks) restored IgM and IgG response to Pnx23 vaccine but not to Prev-13. This suggests that GH could exert distinct impacts upon spenic areas.
Furthermore, after intranasal instillation of a non-lethal dose (defined by the full clearance by WT C57BL/6 mice after 24h) of serotype 1 S.pneumoniae, Ghrh-/- mice exhibited a dramatic susceptibility. This was proved by a marked time-dependent increase in pulmonary bacterial, a septicemia already 24h after infection and a survival limit of 72h. We also observed a dramatic decrease in lung B- and T-cell populations and an increase in proportion of inflammatory macrophages. By contrast, wild-type and heterozygote mice completely cleared S.pneumoniae infection after 24h.
In conclusion, our data show without ambiguity that the somatotrope GHRH/GH/IGF-1 axis plays an important and unsuspected role in defense against S.Pneumoniae.
Giga-Infection, Immunity and Inflammation
F.R.S.-FNRS of Belgium ; Research Concerted Action Somatosthym of the Brussels-Wallonia Federation
Researchers ; Professionals ; Students

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