Article (Scientific journals)
Identification of proteins from wild cardoon flowers (Cynara cardunculus L.) by a proteomic approach
Ben Amira, Amal; Bauwens, Julien; De Pauw, Edwin et al.
2017In Journal of Chemical Biology, 10, p. 25-33
Peer reviewed
 

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Keywords :
Wild cardoon; Protein extraction; 2D gel electrophoresis; Proteomic; MALDI-TOFMS; Cardosin
Abstract :
[en] Proteomic approach was applied to identify total proteins, particularly the enzymatic content, from wild cardoon flowers. As the selection of an appropriate sample preparation method is the key for getting reliable results, two different extraction/precipitation methods (trichloroacetic acid and phenol/ammonium acetate) were tested on fresh and lyophilized flowers. After two-dimensional electrophoresis (2D–E) separations, a better protein pattern was obtained after phenol extraction from lyophilized flowers. Only 46 % of the total analyzed spots resulted in a protein identification by mass spectrometry MALDI-TOF. Four proteases (cardosins A, E, G, and H), which have become a subject of great interest in dairy technology, were identified. They presented molecular weights and isoelectric points very close and high levels of homology between matched peptides sequences. The absence of the other cardosins (B, C, D, and F) could be an advantage, as it reduces the excessive proteolytic activity that causes bitter flavors and texture defects, during cheese making.
Disciplines :
Food science
Author, co-author :
Ben Amira, Amal
Bauwens, Julien ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Entomologie fonctionnelle et évolutive
De Pauw, Edwin  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Besbes, Souhail
Attia, Hamadi
Francis, Frédéric  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Entomologie fonctionnelle et évolutive
Blecker, Christophe ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Science des alim. et formul.
Language :
English
Title :
Identification of proteins from wild cardoon flowers (Cynara cardunculus L.) by a proteomic approach
Publication date :
2017
Journal title :
Journal of Chemical Biology
ISSN :
1864-6158
eISSN :
1864-6166
Publisher :
Springer, Berlin, Germany
Volume :
10
Pages :
25-33
Peer reviewed :
Peer reviewed
Available on ORBi :
since 10 March 2017

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