Reference : Study of Mezoneuron benthamianum, a plant traditionally used against malaria in Guinea
Scientific congresses and symposiums : Poster
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/202058
Study of Mezoneuron benthamianum, a plant traditionally used against malaria in Guinea
English
Tchinda Tiabou, Alembert mailto [Université de Liège > Département de pharmacie > Pharmacognosie >]
Loua, Jean mailto [Research and Valorization Center on Medicinal Plants of Dubreka, Dubreka, Guinea > > > >]
Esters, Virginie mailto [Université de Liège > Département de pharmacie > Pharmacognosie >]
Cieckiewicz, Ewa mailto [Université de Liège > Département de pharmacie > Pharmacognosie >]
Ledoux, Allison mailto [Université de Liège > Département de pharmacie > Pharmacognosie >]
Angenot, Luc mailto [Université de Liège > Département de pharmacie > Département de pharmacie >]
Tits, Monique mailto [Université de Liège > Département de pharmacie > Département de pharmacie >]
Baldé, Aliou M mailto [Departement of Pharmacy, University Gamal Abdel Nasser of Conakry, Conakry, Guinea > > > >]
Frederich, Michel mailto [Université de Liège > Département de pharmacie > Pharmacognosie >]
Jansen, Olivia mailto [Université de Liège > Département de pharmacie > Pharmacognosie >]
Jul-2016
A0
Yes
No
International
9th Joint Natural Products Conference 2016 Copenhagen
24 au 27 juillet 2016
Copenhagen
Danemark
[en] Malaria ; Traditional medicine ; Mezoneuron ; Guinea
[en] Despite some improvements in malaria control, this parasitic disease remains a major public health problem in many African countries, causing about 400 000 deaths/year through the continent, mainly by children under the age of five (WHO, 2015).
In Guinea, the leaves of Mezoneuron benthamianum Baill. are traditionally used to treat malaria (Traore et al., 2013) and showed a good antiplasmodial activity in an antiprotozoal in vitro screening (Traore et al., 2014), as well as promising results in a preliminary small-scale ethnomedical study (unpublished data), encouraging us to continue the study of this plant.
The aim of this work was to evaluate the activity of M. benthamianum leaves extracts against P. falciparum using an in vitro test model (p-LDH assay) and to undertake a bio-guided fractionation to identify the compounds responsible for the activity.
Hydroethanolic extracts (70% v/v) of M. benthamianum leaves showed a moderate in vitro activity against P. falciparum 3D7, with IC50 = 22.5 – 32.6 µg/ml, depending on the batch; while a dark precipitate formed during ethanol evaporation showed higher activity (IC50 = 6,5µg/ml). The bioguided fractionation was performed on this most active fraction and allowed the isolation of three diterpens, two flavonoids, resveratrol, gallic acid and its ethylester, β-sitosterol glucoside and pheophorbide derivatives.
The active compounds belong to several phytochemical classes, including flavonoids, pheophorbide and gallic acid derivatives, contributing together to the global antiplasmodial activity of the hydroalcoholic extract against P. falciparum parasite.
This study gives some concrete evidence to support the ethnopharmacological use of Mezoneuron benthamianum leaves extract in the management of malaria. The active compounds can be further studied for their antiplasmodial potential, as well as their suitability to be used as quality markers for the standardization of this herbal drug from the Guinean traditional pharmacopeia.
http://hdl.handle.net/2268/202058

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
mezoneuron_poster_2016_copenhague.pdfAuthor postprint1.39 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.