PAO; polyphosphate detection; single cell technologies
Abstract :
[en] Phosphate minerals have long been used for the production of phosphorus-based chemicals
used in many economic sectors. However, these resources are not renewable and the natural
phosphate stocks are decreasing. In this context, the research of new phosphate sources has
become necessary. Many types of wastes contain non-negligible phosphate concentrations, such
as wastewater. In wastewater treatment plants, phosphorus is eliminated by physicochemical
and/or biological techniques. In this latter case, a specific microbiota, phosphate accumulating
organisms (PAOs), accumulates phosphate as polyphosphate. This molecule can be considered as an alternative phosphate source, and is directly extracted from wastewater generated by human activities. This review focuses on the techniques which can be applied to enrich and try to isolate these PAOs, and to detect the presence of polyphosphate in microbial cells.
Disciplines :
Biotechnology
Author, co-author :
Tarayre, Cédric ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Nguyen, Huu-Thanh
Brognaux, Alison ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Delepierre, Anissa ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
De Clercq, Lies
Charlier, Raphaëlle
Michels, Evi
Meers, Erik
Delvigne, Frank ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Language :
English
Title :
Characterisation of Phosphate Accumulating Organisms and Techniques for Polyphosphate Detection: A Review
Publication date :
31 May 2016
Journal title :
Sensors
ISSN :
1424-8220
eISSN :
1424-3210
Publisher :
Molecular Diversity Preservation International (MDPI), Basel, Switzerland
Wilfert, P.; Kumar, P.S.; Korving, L.; Witkamp, G.-J.; van Loosdrecht, M.C.M. The relevance of phosphorusand iron chemistry to the recovery of phosphorus from wastewater: A review. Environ. Sci. Technol. 2015, 49,9400–9414.
Schoumans, O.F.; Bouraoui, F.; Kabbe, C.; Oenema, O.; van Dijk, K.C. Phosphorus management in Europe ina changing world. Ambio 2015, 44, 180–192.
Hirota, R.; Kuroda, A.; Kato, J.; Ohtake, H. Bacterial phosphate metabolism and its application to phosphorusrecovery and industrial bioprocesses. J. Biosci. Bioeng. 2010, 109, 423–432.
Powell, N. Biological Phosphorus Removal by Microalgae in Waste Stabilisation Ponds; Massey University:Palmerston North, New Zealand, 2009.
Ye, Y.; Gan, J.; Hu, B. Screening of Phosphorus-Accumulating Fungi and Their Potential for PhosphorusRemoval fromWaste Streams. Appl. Biochem. Biotechnol. 2015, 177, 1127–1136.
Bao, L.L.; Li, D.; Li, X.K.; Huang, R.X.; Zhang, J.; Lu, Y.; Xia, G.Q. Phosphorus accumulation by bacteriaisolated from a continuous-flow two-sludge system. J. Environ. Sci. 2007, 19, 391–395.
Kulakovskaya, T.V.; Vagabov, V.M.; Kulaev, I.S. Inorganic polyphosphate in industry, agriculture andmedicine: Modern state and outlook. Process. Biochem. 2012, 47, 1–10.
Mao, Y.; Graham, D.W.; Tamaki, H.; Zhang, T. Dominant and Novel Clades of Candidatus Accumulibacterphosphatis in 18 Globally Distributed Full-Scale Wastewater Treatment Plants. Sci. Rep. 2015, 5, 11857.
Oehmen, A.; Lemos, P.C.; Carvalho, G.; Yuan, Z.; Keller, J.; Blackall, L.L.; Reis, M.A.M. Advances in enhancedbiological phosphorus removal: From micro to macro scale. Water Res. 2007, 41, 2271–2300.
Morohoshi, T.; Yamashita, T.; Kato, J.; Ikeda, T.; Takiguchi, N.; Ohtake, H.; Kuroda, A. A Method for ScreeningPolyphosphate-Accumulating Mutants Which Remove Phosphate Efficiently from SyntheticWastewater. J. Biosci. Bioeng. 2003, 95, 637–640.
Chaudhry, V.; Nautiyal, C.S. A high throughput method and culture medium for rapid screening of phosphateaccumulating microorganisms. Bioresour. Technol. 2011, 102, 8057–8062.
Aravind, J.; Saranya, T.; Kanmani, P. Optimizing the Production of Polyphosphate from AcinetobacterTowneri. Glob. J. Environ. Sci. Manag. 2015, 1, 63–70.
Khoi, L.Q. Isolation and Phylogenetic Analysis of Polyphosphate Accumulating Organisms inWater andSludge of Intensive Catfish Ponds in the Mekong Delta, Vietnam. Am. J. Life Sci. 2013, 1, 61.
Naili, O.; Benounis, M.; Benammar, L. Screening of Bacteria Isolated from Activated Sludges for PhosphateRemoval fromWastewater. J. Appl. Environ. Biol. Sci. 2015, 5, 1–5.
Yuan, Z.; Pratt, S.; Batstone, D.J. Phosphorus recovery from wastewater through microbial processes.Curr. Opin. Biotechnol. 2012, 23, 878–883.
Tchobanoglous, G.; Stensel, D.; Tsuchihashi, R.; Burton, F. Wastewater Engineering: Treatment and ResourceRecovery; Eddy, M., Ed.; McGraw-Hill Education-Europe: London, UK, 2013.
Abdulsada, Z.K. Evaluation of Microalgae for Secondary and Tertiary Wastewater Treatment. Ph.D. Thesis,Carleton University Ottawa, Ottawa, ON, Canada, 2014.
Eixler, S.; Selig, U.; Karsten, U. Extraction and detection methods for polyphosphate storage in autotrophicplanktonic organisms. Hydrobiologia 2005, 533, 135–143.
Serafim, L.S.; Lemos, P.C.; Levantesi, C.; Tandoi, V.; Santos, H.; Reis, M.A.M. Methods for detectionand visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms.J. Microbiol. Methods 2002, 51, 1–18.
Hupfer, M.; Glöss, S.; Schmieder, P.; Grossart, H.P. Methods for detection and quantification of polyphosphateand polyphosphate accumulating microorganisms in aquatic sediments. Int. Rev. Hydrobiol. 2008, 93, 1–30.
Rao, N.N.; Gómez-García, M.R.; Kornberg, A. Inorganic polyphosphate: Essential for growth and survival.Annu. Rev. Biochem. 2009, 78, 605–647.
Majed, N.; Chernenko, T.; Diem, M.; Gu, A.Z. Identification of functionally relevant populations in enhancedbiological phosphorus removal processes based on intracellular polymers profiles and insights into themetabolic diversity and heterogeneity. Environ. Sci. Technol. 2012, 46, 5010–5017.
Majed, N.; Li, Y.; Gu, A.Z. Advances in techniques for phosphorus analysis in biological sources. Curr. Opin. Biotechnol. 2012, 23, 852–859.
Ezawa, T.; Smith, S.E.; Smith, F.A. Differentiation of polyphosphate metabolism between the extra and intraradical hyphae of arbuscular mycorrhizal fungi. New Phytol. 2001, 149, 555–563.
Pandolfi, D.; Dumas, M.D. Caractérisation Morphologique et Physiologique de la Biomasse des Boues Activées parAnalyse d’ Images; Institut National Polytechnique de Lorraine: Nancy, France, 2006.
Lorenz, B.; Münkner, J.; Oliveira, M.P.; Leitão, J.M.; Müller, W.E.; Schröder, H.C. A novel method fordetermination of inorganic polyphosphates using the fluorescent dye fura-2. Anal. Biochem. 1997, 246,176–184.
Günther, S.; Trutnau, M.; Kleinsteuber, S.; Hause, G.; Bley, T.; Röske, I.; Harms, H.; Müller, S. Dynamicsof polyphosphate-accumulating bacteria in wastewater treatment plant microbial communities detectedvia DAPI (41,61-diamidino-2-phenylindole) and tetracycline labeling. Appl. Environ. Microbiol. 2009, 75,2111–2121.
Günther, S.; Röske, I.; Bley, T. Population Structure and Dynamics of Polyphosphate Accumulating Organisms in aCommunal Wastewater Treatment Plant; Technischen Universität Dresden: Dresden, Germany, 2011.
Jimenez-Nuñez, M.D.; Moreno-Sanchez, D.; Hernandez-Ruiz, L.; Benítez-Rondán, A.; Ramos-Amaya, A.; Rodríguez-Bayona, B.; Medina, F.; Brieva, J.A.; Ruiz, F.A. Myeloma cells contain high levels of inorganicpolyphosphate which is associated with nucleolar transcription. Haematologica 2012, 97, 1264–1271.
Günther, S.; Hübschmann, T.; Rudolf, M.; Eschenhagen, M.; Röske, I.; Harms, H.; Müller, S. Fixationprocedures for flow cytometric analysis of environmental bacteria. J. Microbiol. Methods 2008, 75, 127–134.
Miyauchi, R.; Oki, K.; Aoi, Y.; Tsuneda, S. Diversity of nitrite reductase genes in “Candidatus accumulibacterphosphatis”-dominated cultures enriched by flow-cytometric sorting. Appl. Environ. Microbiol. 2007, 73,5331–5337.
Günther, S.; Koch, C.; Hübschmann, T.; Röske, I.; Müller, R.A.; Bley, T.; Harms, H.; Müller, S. Correlationof community dynamics and process parameters as a tool for the prediction of the stability of wastewatertreatment. Environ. Sci. Technol. 2012, 46, 84–92.
Mehlig, L.; Petzold, M.; Heder, C.; Gunther, S.; Muller, S.; Eschenhagen, M.; Roske, I.; Roske, K. Biodiversityof Polyphosphate Accumulating Bacteria in Eight WWTPs with Different Modes of Operation. J. Environ. Eng.2013, 139, 1089–1098.
Chen, H.; Wang, D.; Li, X.; Yang, Q.; Luo, K. Biological phosphorus removal from real wastewater in asequencing batch reactor operated as aerobic/extended-idle regime. Biochem. Eng. J. 2013, 77, 147–153.
Albertsen, M.; Benedicte, L.; Hansen, S.; Saunders, A.M.; Nielsen, P.H. A metagenome of a full-scale microbialcommunity carrying out enhanced biological phosphorus removal. ISME J. 2012, 2012, 1094–1106.
Broch, S.P. Operation and Control of SBR Processes for Enhanced Biological Nutrient Removal from Wastewater;Universitat de Girona: Girona, Spain, 2008.
Burow, L.C.; Mabbett, A.N.; Blackall, L.L. Anaerobic glyoxylate cycle activity during simultaneous utilizationof glycogen and acetate in uncultured Accumulibacter enriched in enhanced biological phosphorus removalcommunities. ISME J. 2008, 2, 1040–1051.
De Kreuk, M.K.; Heijnen, J.J.; van Loosdrecht, M.C.M. Simultaneous COD, nitrogen, and phosphate removalby aerobic granular sludge. Biotechnol. Bioeng. 2005, 90, 761–769.
Liu, W.T.; Nielsen, A.T.; Wu, J.H.; Tsai, C.S.; Matsuo, Y.; Molin, S. In situ identification of polyphosphate and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removalprocess. Environ. Microbiol. 2001, 3, 110–122.
Chung, J.; Kim, Y.; Lee, D.; Shim, H.; Kim, J. Characteristics of Denitrifying Phosphate AccumulatingOrganisms in an Anaerobic-Intermittently Aerobic Process. Environ. Eng. Sci. 2006, 23, 981–993.
Kim, J.M.; Lee, H.J.; Kim, S.Y.; Song, J.J.; Park, W.; Jeon, C.O. Analysis of the fine-scale population structureof “candidatus accumulibacter phosphatis” in enhanced biological phosphorus removal sludge, usingfluorescence in situ hybridization and flow cytometric sorting. Appl. Environ. Microbiol. 2010, 76, 3825–3835.
Schroeder, S.; Ahn, J.; Seviour, R.J. Ecophysiology of polyphosphate-accumulating organisms andglycogen-accumulating organisms in a continuously aerated enhanced biological phosphorus removalprocess. J. Appl. Microbiol. 2008, 105, 1412–1420.
Breus, N.A.; Ryazanova, L.P.; Suzina, N.E.; Kulakovskaya, N.V.; Valiakhmetov, A.Y.; Yashin, V.A.; Sorokin, V.V.; Kulaev, I.S. Accumulation of inorganic polyphosphates in Saccharomyces cerevisiae undernitrogen deprivation: Stimulation by magnesium ions and peculiarities of localization. Microbiology 2011, 80,624–630.
Breus, N.A.; Ryazanova, L.P.; Dmitriev, V.V.; Kulakovskaya, T.V.; Kulaev, I.S. Accumulation of phosphateand polyphosphate by Cryptococcus humicola and Saccharomyces cerevisiae in the absence of nitrogen.FEMS Yeast Res. 2012, 12, 617–624.
Smith, S.A.; Morrissey, J.H. Sensitive fluorescence detection of polyphosphate in polyacrylamide gels using41,6-diamidino-2-phenylindol. Electrophoresis 2007, 28, 3461–3465.
Cosmidis, J.; Benzerara, K.; Menguy, N.; Arning, E. Microscopy evidence of bacterial microfossils inphosphorite crusts of the Peruvian shelf: Implications for phosphogenesis mechanisms. Chem. Geol. 2013,359, 10–22.
Ge, H.; Batstone, D.J.; Keller, J. Biological phosphorus removal from abattoir wastewater at very short sludgeages mediated by novel PAO clade Comamonadaceae. Water Res. 2015, 69, 173–182.
Rivadeneyra, A.; Gonzalez-Martinez, A.; Gonzalez-Lopez, J.; Martin-Ramos, D.; Martinez-Toledo, M.V.; Rivadeneyra, M.A. Precipitation of phosphate minerals by microorganisms isolated from a fixed-biofilmreactor used for the treatment of domestic wastewater. Int. J. Environ. Res. Public Health 2014, 11, 3689–3704.
Malley, D.F.; Lockhart, L.; Wilkinson, P.; Hauser, B. Determination of carbon, carbonate, nitrogen, andphosphorus in freshwater sediments by near-infrared reflectance spectroscopy: Rapid analysis and a checkon conventional analytical methods. J. Paleolimnol. 2000, 24, 415–425.
Schuster, K.C.; Urlaub, E.; Gapes, J.R. Single-cell analysis of bacteria by Raman microscopy: Spectralinformation on the chemical composition of cells and on the heterogeneity in a culture. J. Microbiol. Methods 2000, 42, 29–38.
Majed, N.; Matthäus, C.; Diem, M.; Gu, A.Z. Evaluation of intracellular polyphosphate dynamics in enhancedbiological phosphorus removal process using Raman microscopy. Environ. Sci. Technol. 2009, 43, 5436–5442.
Majed, N.; Gu, A.Z. Application of Raman microscopy for simultaneous and quantitative evaluation ofmultiple intracellular polymers dynamics functionally relevant to enhanced biological phosphorus removalprocesses. Environ. Sci. Technol. 2010, 44, 8601–8608.
Comolli, L.R.; Kundmann, M.; Downing, K.H. Characterization of intact subcellular bodies in whole bacteriaby cryo-electron tomography and spectroscopic imaging. J. Microsc. 2006, 223, 40–52.
Saito, K.; Ohtomo, R.; Kuga-Uetake, Y.; Aono, T.; Saito, M. Direct labeling of polyphosphate at theultrastructural level in Saccharomyces cerevisiae by using the affinity of the polyphosphate binding domainof Escherichia coli exopolyphosphatase. Appl. Environ. Microbiol. 2005, 71, 5692–5701.
Forbes, C.M.; O’Leary, N.D.; Dobson, A.D.; Marchesi, J.R. The contribution of “omic”-based approaches tothe study of enhanced biological phosphorus removal microbiology: Minireview. FEMS Microbiol. Ecol. 2009,69, 1–15.
Wilmes, P.; Wexler, M.; Bond, P.L. Metaproteomics provides functional insight into activated sludgewastewater treatment. PLoS ONE 2008, 3, e1778.
Wexler, M.; Richardson, D.J.; Bond, P.L. Radiolabelled proteomics to determine differential functioning ofAccumulibacter during the anaerobic and aerobic phases of a bioreactor operating for enhanced biologicalphosphorus removal. Environ. Microbiol. 2009, 11, 3029–3044.