Profil

Brognaux Alison

See author's contact details
Main Referenced Co-authors
Delvigne, Frank  (39)
Thonart, Philippe  (31)
Francis, Frédéric  (12)
Sorensen, Soren (11)
Delepierre, Anissa  (9)
Main Referenced Keywords
Escherichia coli (5); membrane permeability (5); phenotypic heterogeneity (5); bioreactor (4); mini-bioreactors (4);
Main Referenced Unit & Research Centers
Center for Education and Research on Macromolecules (CERM) (5)
Centre Wallon de Biologie Industrielle (1)
Unité de bio-industries / Gembloux Agro Bio-Tech / Université de Liège (1)
Université de Liège - Gembloux Agro Bio Tech (1)
Main Referenced Disciplines
Biotechnology (40)
Chemistry (6)
Materials science & engineering (4)
Microbiology (3)
Biochemistry, biophysics & molecular biology (1)

Publications (total 52)

The most downloaded
2552 downloads
Brognaux, A. (2008). Mise en évidence de l'impact de l'environnement extracellulaire généré au sein des bioréacteurs sur la dynamique des populations microbiennes: cas de la culture fed-batch d'Escherichia coli [Master’s dissertation, Gembloux Agro Bio Tech - Université de Liège]. ORBi-University of Liège. https://orbi.uliege.be/handle/2268/63987 https://hdl.handle.net/2268/63987

The most cited

61 citations (Scopus®)

Tarayre, C., Nguyen, H.-T., Brognaux, A., Delepierre, A., De Clercq, L., Charlier, R., Michels, E., Meers, E., & Delvigne, F. (31 May 2016). Characterisation of Phosphate Accumulating Organisms and Techniques for Polyphosphate Detection: A Review. Sensors, 16 (797), 1-14. doi:10.3390/s16060797 https://hdl.handle.net/2268/199402

Tarayre, C., Charlier, R., Delepierre, A., Brognaux, A., Bauwens, J., Francis, F., DERMIENCE, M., Lognay, G., Taminiau, B., Daube, G., Compère, P., Meers, E., Michels, E., & Delvigne, F. (2017). Looking for phosphate-accumulating bacteria in activated sludge processes : a multidisciplinary approach. Environmental Science and Pollution Research. doi:10.1007/s11356-017-8490-y
Peer Reviewed verified by ORBi

Tarayre, C., Nguyen, H.-T., Brognaux, A., Delepierre, A., De Clercq, L., Charlier, R., Michels, E., Meers, E., & Delvigne, F. (31 May 2016). Characterisation of Phosphate Accumulating Organisms and Techniques for Polyphosphate Detection: A Review. Sensors, 16 (797), 1-14. doi:10.3390/s16060797
Peer Reviewed verified by ORBi

Delepierre, A., Brognaux, A., Baert, J., Tarayre, C., Bauwens, J., Francis, F., & Delvigne, F. (20 May 2016). Study of microbial phenotypic heterogeneity under bioprocess conditions using « single-cell » techniques [Poster presentation]. Journée TERRA.

Delepierre, A., Brognaux, A., Bauwens, J., Francis, F., & Delvigne, F. (12 May 2016). Single cell analysis of Escherichia coli outer membrane porin composition in response to nutrient depletion [Poster presentation]. Conférences « Chimie verte et Biotechnologie blanche », Gembloux, Belgium.

Baert, J., Delepierre, A., Brognaux, A., Toye, D., & Delvigne, F. (19 January 2016). Caractérisation de l’hétérogénéité phénotypique des populations microbiennes : vers de nouvelles stratégies pour l’optimisation des bioprocédés [Paper presentation]. Journées Jeunes Chercheurs Condorcet, Reims, France.

Baert, J., Delepierre, A., Brognaux, A., & Delvigne, F. (01 October 2015). Single cell analysis of the activity of the electron transport chain : a promising way to assess the effect of metabolic engineering strategies [Paper presentation]. 3rd European Congress of Applied Biotechnology.

Brognaux, A., Delepierre, A., Baert, J., Pêcheux, H., Madsen, J., Riber, L., Sorensen, S., & Delvigne, F. (01 October 2015). Impact of metabolic engineering strategies on phenotypic heterogeneity [Paper presentation]. 3rd European Congress of Applied Biotechnology - ECAB3, Nice, France.

Brognaux, A., Delepierre, A., Pecheux, H., Baert, J., Madsen, J., Riber, L., Guillermo, G., Lara, A., Sorensen, S., & Delvigne, F. (21 July 2015). Impact of phenotypic heterogeneity and metabolic specialisation on metabolic engineering strategies: case of study of E.coli as a representative microbial cell factory [Paper presentation]. 6th International Conference on Analysis of Microbial Cells at the Single Cell Level, Retz, Austria.

Baert, J., Kinet, R., Brognaux, A., Delepierre, A., Telek, S., Sorensen, S. J., Riber, L., Fickers, P., & Delvigne, F. (2015). Phenotypic variability in bioprocessing conditions can be tracked on the basis of on-line flow cytometry and fits to a scaling law. Biotechnology Journal. doi:10.1002/biot.201400537
Peer Reviewed verified by ORBi

Baert, J., Brognaux, A., & Delvigne, F. (18 September 2014). Flow cytometry metabolic fingerprinting : an efficient tool for the characterization of microbial metabolic activity at a single cell resolution [Poster presentation]. 19th Conference on Food Microbiology.

Brognaux, A., Francis, F., Twizere, J.-C., Thonart, P., & Delvigne, F. (08 September 2014). Characterization of the scale-down effect on the extracellular proteome of Escherichia coli and correlation with membrane permeability [Poster presentation]. ESBES 2014, Lille, France.

Delvigne, F., Gorret, N., Molina-Jouve, C., Zune, Q., & Brognaux, A. (September 2014). Implications of microbial phenotypic heterogeneity in large-scale bioprocessing conditions [Paper presentation]. European symposium on biochemical engineering science ESBES, Lille, France.

Brognaux, A., Han, S., Sorensen, S., Lebeau, F., Thonart, P., & Delvigne, F. (03 April 2014). Use of on-line flow cytometry for the characterization of microbial stress dynamics during the bioprocess [Paper presentation]. 3rd BioProScale Symposium / Inhomogeneities in large-scale bioprocesses - System biology and process dynamics, Berlin, Germany.

Brognaux, A. (2014). Utilisation de biocapteurs microbiens GFP pour la caractérisation des performances des bioréacteurs [Doctoral thesis, ULiège - Université de Liège]. ORBi-University of Liège. https://orbi.uliege.be/handle/2268/164267

Brognaux, A., Francis, F., Twizere, J.-C., Thonart, P., & Delvigne, F. (2014). Scale-down effect on the extracellular proteome of Escherichia coli: correlation with membrane permeability and modulation according substrate heterogeneities. Bioprocess and Biosystems Engineering. doi:10.1007/s00449-013-1119-8
Peer Reviewed verified by ORBi

Brognaux, A., Han, S., Sorensen, S., Lebeau, F., Thonart, P., & Delvigne, F. (07 February 2014). Use of on-line flow cytometry for the characterization of microbial stress dynamics during the bioprocess [Poster presentation]. 19th National Symposium on Applied Biological Sciences, Gembloux, Belgium.

Tarayre, C., Brognaux, A., Bauwens, J., Brasseur, C., Mattéotti, C., Millet, C., Destain, J., Vandenbol, M., Portetelle, D., De Pauw, E., Haubruge, E., Francis, F., & Thonart, P. (2013). Short communication - Isolation of amylolytic, xylanolytic, and cellulolytic microorganisms extracted from the gut of the termite Reticulitermes santonensis by means of a micro-aerobic atmosphere. World Journal of Microbiology and Biotechnology.
Peer Reviewed verified by ORBi

Brognaux, A., Han, S., Soren, S., Lebeau, F., Thonart, P., & Delvigne, F. (15 November 2013). Use of on-line flow cytometry for the characterization of physiological behavior in stress conditions during the bioprocess [Poster presentation]. Journée Scientifique - Ecole doctorale Thématique en Génie des Procédés "Procédés et matériaux durables", Liège, Belgium.

Brognaux, A., Zune, Q., Han, S., Sorensen, S., Lebeau, F., Thonart, P., & Delvigne, F. (08 October 2013). Use of on-line flow cytometry to detect segregation in the microbial population [Poster presentation]. 14ème congrès de la Société Française en Génie des Procédés, Lyon, France.

Tarayre, C., Brognaux, A., Brasseur, C., Bauwens, J., Millet, C., Mattéotti, C., Destain, J., Vandenbol, M., Portetelle, D., De Pauw, E., Haubruge, E., Francis, F., & Thonart, P. (2013). Isolation and Cultivation of a Xylanolytic Bacillus subtilis Extracted from the Gut of the Termite Reticulitermes santonensis. Applied Biochemistry and Biotechnology. doi:10.1007/s12010-013-0337-5
Peer Reviewed verified by ORBi

Brognaux, A., Han, S., Sorensen, S., Lebeau, F., Thonart, P., & Delvigne, F. (24 April 2013). On-line flow cytometry to detect cell growth rate and membrane damage during bioprocess: focus on the segregation of the microbial population [Paper presentation]. 2nd european congress of applied biotechnology, La Haye, Netherlands.

Brognaux, A., Francis, F., Twizere, J.-C., Thonart, P., & Delvigne, F. (23 April 2013). Characterization of the extracellular proteome of Escherichia coli according to the scale-up of bioprocesses and correlation with membrane permeability [Poster presentation]. 2nd European Congress of Applied Biotechnology, La Haye, Netherlands.

Brognaux, A., Han, S., Sorensen, S., Lebeau, F., Thonart, P., & Delvigne, F. (08 February 2013). On-line flow cytometry profiling of Escherichia coli stress response [Paper presentation]. 18th Symposium on Applied and Biological Sciences, Gent, Belgium.

Brognaux, A., Shanshan, H., Sorensen, S., Lebeau, F., Thonart, P., & Delvigne, F. (2013). On-line profiling of Escherichia coli stress response. In Communications in Agricultural and Applied Biological Sciences.
Peer reviewed

Brognaux, A., Neubauer, P., Twizere, J.-C., Francis, F., Gorret, N., Thonart, P., & Delvigne, F. (2013). Direct and indirect use of GFP whole cell biosensors for the assessment of bioprocess performances: design of milliliter scale-down bioreactors. Biotechnology Progress, 29 (1), 48-59. doi:10.1002/btpr.1660
Peer Reviewed verified by ORBi

Brognaux, A., Han, S., Sorensen, S. J., Lebeau, F., Thonart, P., & Delvigne, F. (2013). A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors. Microbial Cell Factories, 12 (100), 1-14. doi:10.1186/1475-2859-12-100
Peer Reviewed verified by ORBi

Delvigne, F., Brognaux, A., Han, S., Sorensen, S., & Thonart, P. (2013). Use of GFP whole cell microbial biosensors for the investigation of the scale-up and scale-down effect on biopharmaceutical processes. Momentum press.

Han, S., Delvigne, F., Brognaux, A., Charbon, G., & Sorensen, S. (2013). Design of growth-dependent biosensors based on destabilized GFP for the detection of physiological behavior of Escherichia coli in heterogeneous bioreactors. Biotechnology Progress, 29 (2), 553-563. doi:10.1002/btpr.1694
Peer Reviewed verified by ORBi

Brognaux, A., Bugge, J., Schwartz, F., Thonart, P., Telek, S., & Delvigne, F. (2013). Real-time monitoring of cell viability and cell density on the basis of a three dimensional optical reflectance method (3D-ORM): investigation of the effect of sub-lethal and lethal injuries. Journal of Industrial Microbiology and Biotechnology. doi:10.1007/s10295-013-1271-9
Peer Reviewed verified by ORBi

Zune, Q., Toye, D., Delvigne, F., Brognaux, A. (Other coll.), Ongena, M. (Other coll.), & Thonart, P. (Other coll.). (2013). Biofilm formation on metal structured packing for the production of high added value biomolecules. Récents Progrès en Génie des Procédés.
Peer reviewed

Brognaux, A., Neubauer, P., Twizere, J.-C., Thonart, P., & Delvigne, F. (18 May 2012). Development of mini scale-down platform based on the response of GFP microbial biosensors [Poster presentation]. Biosensors 2012, Cancun, Mexico.

Brognaux, A., Neubauer, P., Twizere, J.-C., Thonart, P., & Delvigne, F. (15 March 2012). Use of microbial biosensors to detect substrate heterogeneities at the single cell level and assess microbial viability: Validation of a mini-bioreactor platform [Paper presentation]. 2nd BioProScale "Inhomogeneities in large scale bioprocess: system biology and process dynamics".

Brognaux, A., Neubauer, P., Twizere, J.-C., Thonart, P., & Delvigne, F. (10 February 2012). Potentiality of using microbial biosensors for the detection of sub-strate heterogeneities and the assessment of microbial viability in industrial bioreactors: a complete set of experiments in chemostat and scale-down reactors, and elaboration of a mini scale-down platform [Paper presentation]. 17th Symposium on Applied and Biological Sciences, Leuven, Belgium.

Brognaux, A., Nott, K., Richard, G., Laurent, P., Favrelle, A., Jérôme, C., Blecker, C., Wathelet, J.-P., Paquot, M., & Deleu, M. (2012). (Trans)esterification of mannose catalyzed by lipase B from Candida antarctica in an improved reaction medium using co-solvents and molecular sieve. Preparative Biochemistry and Biotechnology, 42 (4), 348-363. doi:10.1080/10826068.2011.622330
Peer Reviewed verified by ORBi

Brognaux, A., Neubauer, P., Twizere, J.-C., Thonart, P., & Delvigne, F. (2012). Potentiality of using microbial biosensors for the detection of substrate heterogeneities and the assessment of microbial viability in industrial bioreactors: a complete set of experiments in chemostat and scale-down reactors, and elaboration of a mini scale-down platform. Communications in Agricultural and Applied Biological Sciences, 77 (1), 3-7.
Peer Reviewed verified by ORBi

Delvigne, F., Brognaux, A., Gorret, N., Delafosse, A., Collignon, M.-L., Toye, D., Crine, M., & Thonart, P. (29 November 2011). Prise en compte l'état de la membrane cellulaire sur la réponse de biocapteurs fluorescents pour la détection de défaut d'écoulement au niveau des bioréacteurs : vers une intégration du "sécrétome" dans la problématique de l'extrapolation [Paper presentation]. 12ème congrès de la Société Française de génie des procédés, Lille, France.

Brognaux, A., Delvigne, F., & Thonart, P. (07 April 2011). Utilization of whole cell Green Fluorescent Protein (GFP) reporter systems as tracers to estimate the bioreactor efficiency [Poster presentation]. 3ème journée de réflexion de l'Ecole Doctorale Thématique en Génie des Procédés: "L'intensification des procédés du point de vue académique et industriel", Bruxelles, Belgium.

Delvigne, F., Brognaux, A., Francis, F., Twizere, J.-C., Gorret, N., Sorensen, S., & Thonart, P. (2011). Green fluorescent protein (GFP) leakage from microbial biosensors provides useful information for the evaluation of the scale-down effect. Biotechnology Journal, 6, 968-978. doi:10.1002/biot.201000410
Peer Reviewed verified by ORBi

Delvigne, F., Brognaux, A., Gorret, N., Sorensen, S., Crine, M., & Thonart, P. (2011). Applicability of GFP Microbial Whole Cell Biosensors to Bioreactor Operations : Mathematical Modeling and Related Experimental Tools. In Biosensors : emerging materials and applications (pp. 601-630). Rijeka, Croatia: Intech.

Delvigne, F., Brognaux, A., Gorret, N., Delafosse, A., Collignon, M.-L., Toye, D., Crine, M., & Thonart, P. (2011). Taking into account cell membrane permeability to account for the response of GFP microbial biosensors : integrating the secretome in the scaling-up procedures. Récents Progrès en Génie des Procédés, 101, 1-7.
Peer reviewed

Delvigne, F.* , Brognaux, A.* , Gorret, N., Neubauer, P., Delafosse, A., Collignon, M.-L., Toye, D., Crine, M., Boxus, M., & Thonart, P. (2011). Characterization of the response of GFP microbial biosensors sensitive to substrate limitation in scale-down bioreactors. Biochemical Engineering Journal, 55 (2), 131-139. doi:10.1016/j.bej.2011.03.016
Peer Reviewed verified by ORBi
* These authors have contributed equally to this work.

Brognaux, A., Delvigne, F., & Thonart, P. (08 December 2010). E.coli prpoS::gfp strain as biosensor of glucose heterogeneity inside industrial bioreactors [Poster presentation]. Biowin Day 2010. "Science for business: open innovation, the new model to innovate in health", Louvain-la-neuve, Belgium.

Nott, K., Brognaux, A., Richard, G., Paquot, M., Wathelet, J.-P., Blecker, C., Jérôme, C., & Deleu, M. (14 October 2010). Influence of co-solvents and molecular sieve on mannose enzymatic acylation [Poster presentation]. Journée Annuelle de la Société Royale de Chimie (Belgique), Gembloux, Belgium.

Bauwens, J., Matteotti, C., Brognaux, A., Brasseur, C., Destain, J., Vandenbol, M., Portetelle, D., Thonart, P., De Pauw, E., Haubruge, E., & Francis, F. (08 July 2010). Caractérisation de la diversité des organismes symbiotiques et des activités glycosyl hydrolases dans le tube digestif de Reticulitermes santonensis (Feytaud) par une approche multidisciplinaire [Paper presentation]. CIFE, Louvain-la-Neuve, Belgium.

Favrelle, A., Brognaux, A., Nott, K., Richard, G., Laurent, P., Debuigne, A., Deleu, M., Wathelet, J.-P., Blecker, C., Paquot, M., & Jérôme, C. (18 May 2010). Synthèse enzymatique de surfactants sucrés en milieux organiques et dans le CO2 supercritique [Poster presentation]. XXIII ème Journées du Groupe Français des Glucides, Cap Hornu, France.

Bauwens, J., Matteotti, C., Brognaux, A., Brasseur, C., Destain, J., Vandenbol, M., Portetelle, D., Thonart, P., De Pauw, E., Haubruge, E., & Francis, F. (05 May 2010). Integrated “omics” approaches to investigate the chemical aspects of symbiosis in termites and potential application in ligno-cellulosic use [Paper presentation]. One-Day Symposium on Chemical Entomology, Gembloux, Belgium.

Richard, G., Brognaux, A., Nott, K., Paquot, M., & Wathelet, J.-P. (May 2010). Influence de l'ajout de co-solvant pour l'estérification du D-mannose et de l'acide D-glucuronique assistée par les lipases [Poster presentation]. Club de bioconversion en synthèse organique, Royat, France.

Delvigne, F., Destain, J., Brognaux, A., Kar, T., Lejeune, A., & Thonart, P. (2010). L'extrapolation des bioréacteurs : un problème de génie des procédés ou de physiologie microbienne ? Chimie Nouvelle, 104, 7-14.

Brognaux, A., Favrelle, A., Deleu, M., Richard, G., Nott, K., Debuigne, A., Jérôme, C., Blecker, C., Wathelet, J.-P., & Paquot, M. (15 October 2009). Optimisation de l'acylation enzymatique du mannose par l'utilisation de co-solvants [Poster presentation]. 2ème congrès pluridisciplinaire sur les glucides (Glucidoc 2009), Autrans, France.

Favrelle, A., Brognaux, A., Debuigne, A., Richard, G., Deleu, M., Nott, K., Wathelet, J.-P., Blecker, C., Paquot, M., & Jérôme, C. (15 October 2009). Synthèse enzymatique de surfactants sucrés dans le CO2 supercritique [Poster presentation]. 2ème congrès pluridisciplinaire sur les glucides (Glucidoc 2009), Autrans, France.

Favrelle, A., Brognaux, A., Debuigne, A., Deleu, M., Nott, K., Wathelet, J.-P., Blecker, C., Paquot, M., & Jérôme, C. (07 July 2009). Enzymatic modifications of sugar in supercritical carbon dioxide [Poster presentation]. Biotrans 2009, 9th International Symposium on Biocatalysis and Biotransformations, Berne, Switzerland.

Brognaux, A. (2008). Mise en évidence de l'impact de l'environnement extracellulaire généré au sein des bioréacteurs sur la dynamique des populations microbiennes: cas de la culture fed-batch d'Escherichia coli [Master’s dissertation, Gembloux Agro Bio Tech - Université de Liège]. ORBi-University of Liège. https://orbi.uliege.be/handle/2268/63987

Contact ORBi