Garcinia kola; antimalarial-Improved traditional medicine; Analytical marker
Abstract :
[en] African populations use traditional medicines in their initial attempt to treat a range of diseases. Nevertheless, accurate knowledge of the composition of these drugs remains a challenge in terms of ensuring
the health of population and in order to advance towards improved traditional medicines (ITMs). In this
paper chromatographic methods were developed for qualitative and quantitative analyses of a per os
antimalarial ITM containing Garcinia kola.
The identified analytical markers were used to establish TLC and HPLC fingerprints. G. kola seeds were
analysed by HPLC to confirm the identity of the extract used by the Congolese manufacturer in the
ITM. The main compounds (GB1, GB2, GB-1a and Kolaflavanone) were isolated by preparative TLC and
identified by UPLC–MS and NMR. For the quantification of the major compound GB1, a simple and rapid
experimental design was applied to develop an LC method, and then its validation was demonstrated
using the total error strategy with the accuracy profile as a decision tool. The accurate results were
observed within 0.14–0.45 mg/mL range of GB1 expressed as naringenin. The extracts used in several
batches of the analysed oral solutions contained GB1 (expressed as naringenin) within 2.04–2.43%.
Both the fingerprints and the validated LC-DAD were found suitable for the quality control of G. kolabased raw material and finished products, respectively.
Research center :
Centre Interfacultaire de Recherche du Médicament - CIRM
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