Characterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD

Odjo, Djosse Psijus Sylvanus; Bera, François; Jacquet, Nicolas; Richel, Aurore; Malumba Kamba, Paul

doi:10.1002/star.201500281

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Characterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD

Odjo, Djosse Psijus Sylvanus; Bera, François; Jacquet, Nicolas et al.

2016 • In Stärke, 68, p. 1-9

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https://hdl.handle.net/2268/194130

DOI
10.1002/star.201500281

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Keywords :

Corn / Digestion / HPAEC-PAD; Oligosaccharides / Starch

Abstract :

[en] Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric detector (HPAEC-PAD) and two colorimetric approaches (glucose oxidase/ peroxidase assay and the 3,5-dinitrosalicylic acid assay). HPAEC-PAD revealed five major saccharides as the result of in vitro pepsin-pancreatin digestion of corn grain: glucose, isomaltose, maltose, maltotriose, and glucosyl-maltotriose. Concentrations of glucose released as measured by the three methods assessed are similar when the in vitro pepsin-pancreatin digestion is followed by post incubation with amyloglucosidase. This post incubation unfortunately leads to a loss of information about the degree of polymerization of oligosaccharides that can impact their absorption through intestinal tract. HPAEC-PAD gives both qualitative and quantitative information and then seems more suitable for a nutritional appreciation of the digestion of starchy feedstuffs.

Disciplines :

Food science

Author, co-author :

Odjo, Djosse Psijus Sylvanus ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.

Bera, François ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Labo. d'Ingénierie des procédés agro-alim.

Jacquet, Nicolas ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle

Richel, Aurore ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle

Malumba Kamba, Paul ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Labo. d'Ingénierie des procédés agro-alim.

Language :

English

Title :

Characterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD

Publication date :

2016

Journal title :

Stärke

ISSN :

0038-9056

eISSN :

1521-379X

Publisher :

Wiley-VCH

Volume :

Pages :

1-9

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

MAISCVOL

Funders :

Direction Génerale Opérationnelle de l’Agriculture, des Ressources Naturelles et de l’Environnement

Available on ORBi :

since 01 March 2016

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