Characterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD

[en] Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric detector (HPAEC-PAD) and two colorimetric approaches (glucose oxidase/ peroxidase assay and the 3,5-dinitrosalicylic acid assay). HPAEC-PAD revealed five major saccharides as the result of in vitro pepsin-pancreatin digestion of corn grain: glucose, isomaltose, maltose, maltotriose, and glucosyl-maltotriose. Concentrations of glucose released as measured by the three methods assessed are similar when the in vitro pepsin-pancreatin digestion is followed by post incubation with amyloglucosidase. This post incubation unfortunately leads to a loss of information about the degree of polymerization of oligosaccharides that can impact their absorption through intestinal tract. HPAEC-PAD gives both qualitative and quantitative information and then seems more suitable for a nutritional appreciation of the digestion of starchy feedstuffs.

Disciplines :

Food science

Author, co-author :

Odjo, Djosse Psijus Sylvanus ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.

Bera, François ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Labo. d'Ingénierie des procédés agro-alim.

Jacquet, Nicolas ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle

Richel, Aurore ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle

Malumba Kamba, Paul ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Labo. d'Ingénierie des procédés agro-alim.

Language :

English

Title :

Characterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD

Publication date :

2016

Journal title :

Stärke

ISSN :

0038-9056

eISSN :

1521-379X

Publisher :

Wiley-VCH

Volume :

Pages :

1-9

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

MAISCVOL

Funders :

Direction Génerale Opérationnelle de l’Agriculture, des Ressources Naturelles et de l’Environnement

Available on ORBi :

since 01 March 2016

Statistics

Number of views

127 (12 by ULiège)

Number of downloads

1 (0 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

publications

supporting

mentioning

contrasting

Smart Citations

Citing PublicationsSupportingMentioningContrasting

View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

Bibliography

Holmes, R., Carbohydrate digestion and absorption. J. Clin. Pathol. Suppl. (Royal Coll. Pathol.) 1971, 5, 10–13.
Jones, B. J. M., Brown, B. E., Loran, J. S., Edgerton, D., et al. Glucose absorption from starch hydrolysates in the human jejunum. Gut. 1983, 24, 1152–1160.
Dona, A. C., Pages, G., Gilbert, R. G., Kuchel, P. W., Digestion of starch: In vivo and in vitro kinetic models used to characterise oligosaccharide or glucose release. Carbohydr. Polym. 2010, 80, 599–617.
Goñi, I., Garcia-Alonso, A., Saura-Calixto, F. A., Starch hydrolysis procedure to estimate glycemic index. Nutr. Res. 1997, 17, 427–437.
Corradini, C., Cavazza, A., Bignardi, C., High-performance anion-exchange chromatography coupled with pulsed electrochemical detection as a powerful tool to evaluate carbohydrates of food interest: Principles and applications. Int. J. Carbohydr. Chem. 2012, 2012, 487564.
Pico, J., Martínez, M. M., Martín, M. T., Gómez, M., Quantification of carbohydrates in wheat flours with an HPAEC-PAD method. Food Chem. 2015, 173, 674–681.
Goffin, D., Robert, C., Wathelet, B., Blecker, C., et al. Step-forward method of quantitative analysis of enzymatically produced isomaltooligosaccharide preparations by AEC-PAD. Chromatographia 2009, 69, 287–293.
Van Soest Robertson, J. B., Lewes, B. A., Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 1991, 74, 3583–3597.
Malumba, P., Janas, S., Masimango, T., Sindic, M., et al. Influence of drying temperature on the wet-milling performance and the proteins solubility indexes of corn kernels. J. Food Eng. 2009, 95, 393–399.
Morrison, W. R., Laignelet, B., An improved colorimetric procedure for determining apparent amylose and other starches. J. Cereal Sci. 1983, 1, 9–20.
Malumba, P., Odjo, S., Boudry, C., et al. Physicochemical characterization and in vitro assessment of the nutritive value of starch yield from corn dried at different temperatures. Starch/Stärke 2014, 66, 738–748.
Miller, G. L., Use of dinitrosalicylic acid reagent for determination of reducing carbohydrate. Anal. Chem. 1959, 31, 426–428.
McCleary, B., Codd, R., Measurement of (1→3), (1→4) β-D-Glucan in barley and oats: Streamlined enzymic procedure. J. Sci. Food Agr. 1991, 55, 303–312.
Shrivastava, A., Gupta, V., Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chron. Young Sci. 2011, 2, 21–25.
Warren, F. J., Zhang, B., Waltzer, G., et al. The interplay of α-amylase and amyloglucosidase activities on the digestion of starch in in vitro enzymic systems. Carbohydr. Polym. 2015, 117, 192–200.
Saqib, A. A. N., Whitney, P. J., Differential behavior of the dinitrosalicylic acid (DNS) reagent towards mono- and di-saccharide carbohydrates. Biomass Bioenergy 2011, 35, 4748–4750.
Xu, H., Leng, X., Wang, M., Zhang, G., Glucose measurement in the presence of tea polyphenols. Food Anal. Methods 2012, 5, 1027–1032.
Teixeira, R. S. S., Da Silva, A. S. A., Ferreira-Leitão, V. S., Da Silva Bon, E. P., Amino acids interference on the quantification of reducing sugars by the 3, 5-dinitrosalicylic acid assay mislead carbohydrase activity measurements. Carbohydr. Res. 2012, 363, 33–37.
Gurung, N., Ray, S., Bose, S., Rai, V., A broader view: Microbial enzymes and their relevance in industries, medicine, and beyond. Biomed. Res. Int. 2013, 2013, 329121.
Conway, R. L., Hood, L. F., Pancreatic alpha amylase hydrolysis products of modified and unmodified tapioca starches. Starch/Stärke 1976, 28, 341–343.
Sreenath, H. K., Studies on sarch granules digestion by α-Amylase. Starch/Stärke 1992, 44, 61–63.
Barrett K., Brooks H., Boitano H., Barman S., Ganong's Review of Medical Physiology, 23rd ed., McGraw Hill, USA 2010.
Gutierez del Alamo, A., Verstegen, M. W. A., Den Hartog, L. A., et al. Wheat starch digestion rate affects broiler performance. Poult. Sci. 2009, 88, 1666–1675.
Giuberti, G., Gallo, A., Masoero, F., et al. Factors affecting starch utilization in large animal food production system: A review. Starch/Stärke 2014, 66, 72–90.
Van den Borne, J. J. G. C., Schrama, J. W., Heetkamp, M. J. W., et al. Synchronising the availability of amino acids and glucose increases protein retention in pigs. Animal 2007, 1, 666–674.
Pazur, J. H., Ando, T., The Hydrolysis of glucosyl oligosaccharides with α-D-(1-4) and α-d-(1-6) bonds by fungal amyloglucosidase. J. Biol. Chem. 1960, 235, 297–302.
Pazur, J. H., Kleppe, K., The hydrolysis of alpha-D-glucosides by amyloglucosidase from Aspergillus niger. J. Biol. Chem. 1962, 237, 1002–1006.
Fogarty, W. M., Benson CP purification and properties of a thermophilic amyloglucosidase from aspergillus niger. Eur. J. Appl. Microbiol. Biotechnol. 1983, 18, 271–278.
Hiromi, K., Kawai, M., Ono, S., Kinetic studies on gluc-amylase: IV. hydrolysis of isomaltose. J. Biochem. 1966, 59, 476–480.
Cai, L. S., Mechanism and enzymatic contribution to in vitro test method of digestion for maize starches differing in amylose content. J. Agric. Food Chem. 2012, 60, 4379–4387.
Hall, M. B., Determination of dietary starch in animal feeds and pet food by an enzymatic-colorimetric method: Collaborative study. J. AOAC Int. 2015, 98, 397–409.

Similar publications

Sorry the service is unavailable at the moment. Please try again later.