Keywords :
Animals; COS Cells; Cercopithecus aethiops; Gene Expression Regulation; Genomic Imprinting; Intercellular Signaling Peptides and Proteins/metabolism; MicroRNAs/metabolism; Muscle, Skeletal/metabolism; Sheep/genetics; Transfection
Abstract :
[en] BACKGROUND: Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect. RESULTS: We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo. CONCLUSIONS: This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect.
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