Article (Scientific journals)
Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression.
Cheng, Huijun; Xu, Xuewen; Hadfield, Tracy et al.
2014In BMC Genomics, 15, p. 944
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Keywords :
Animals; COS Cells; Cercopithecus aethiops; Gene Expression Regulation; Genomic Imprinting; Intercellular Signaling Peptides and Proteins/metabolism; MicroRNAs/metabolism; Muscle, Skeletal/metabolism; Sheep/genetics; Transfection
Abstract :
[en] BACKGROUND: Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect. RESULTS: We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo. CONCLUSIONS: This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect.
Disciplines :
Genetics & genetic processes
Author, co-author :
Cheng, Huijun ;  Université de Liège - ULiège > Doct. sc. vété. (Bologne)
Xu, Xuewen
Hadfield, Tracy
Cockett, Noelle
Charlier, Carole  ;  Université de Liège > Département des productions animales (DPA) > GIGA-R : Génomique animale
Georges, Michel  ;  Université de Liège > Département des productions animales (DPA) > GIGA-R : Génomique animale
Takeda, Haruko ;  Université de Liège > GIGA-Research
Language :
English
Title :
Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression.
Publication date :
30 October 2014
Journal title :
BMC Genomics
eISSN :
1471-2164
Publisher :
BioMed Central, United Kingdom
Volume :
15
Pages :
944
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 December 2015

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