Keywords :
Amino Acids/analysis; Cephalosporins/metabolism; Chromatography, Affinity; Chromosomes, Bacterial/enzymology; Citrobacter/enzymology; Electrophoresis, Polyacrylamide Gel; Isoelectric Point; Isoenzymes/isolation & purification; Kinetics; Molecular Weight; Penicillins/metabolism; Substrate Specificity; beta-Lactamases/isolation & purification
Abstract :
[en] Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.
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