Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.

Amino Acids/analysis; Cephalosporins/metabolism; Chromatography, Affinity; Chromosomes, Bacterial/enzymology; Citrobacter/enzymology; Electrophoresis, Polyacrylamide Gel; Isoelectric Point; Isoenzymes/isolation & purification; Kinetics; Molecular Weight; Penicillins/metabolism; Substrate Specificity; beta-Lactamases/isolation & purification

Abstract :

[en] Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Amicosante, G.

Oratore, A.

Franceschini, N.

Maccarrone, M.

Strom, R.

Galleni, Moreno ; Université de Liège - ULiège

Frère, Jean-Marie ; Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines

Language :

English

Title :

Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.

Publication date :

1988

Journal title :

Biochemical Journal

ISSN :

0264-6021

eISSN :

1470-8728

Publisher :

Portland Press, United Kingdom

Volume :

254

Issue :

Pages :

885-90

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 01 December 2015

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