Article (Scientific journals)
Biochemical Characterization of the Fez-1 Metallo-Beta-Lactamase of Legionella Gormanii Atcc 33297t Produced in Escherichia Coli
Mercuri, Paola; Bouillenne, Fabrice; Boschi, L. et al.
2001In Antimicrobial Agents and Chemotherapy, 45 (4), p. 1254-62
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Abstract :
[en] The bla(FEZ-1) gene coding for the metallo-beta-lactamase of Legionella (Fluoribacter) gormanii ATCC 33297T was overexpressed via a T7 expression system in Escherichia coli BL21(DE3)(pLysS). The product was purified to homogeneity in two steps with a yield of 53%. The FEZ-1 metallo-beta-lactamase exhibited a broad-spectrum activity profile, with a preference for cephalosporins such as cephalothin, cefuroxime, and cefotaxime. Monobactams were not hydrolyzed. The beta-lactamase was inhibited by metal chelators. FEZ-1 is a monomeric enzyme with a molecular mass of 29,440 Da which possesses two zinc-binding sites. Its zinc content did not vary in the pH range of 5 to 9, but the presence of zinc ions modified the catalytic efficiency of the enzyme. A model of the FEZ-1 three-dimensional structure was built.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Mercuri, Paola ;  Université de Liège > Département des sciences de la vie > Macromolécules biologiques
Bouillenne, Fabrice ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Boschi, L.
Lamotte-Brasseur, Josette 
Amicosante, G.
Devreese, B.
van Beeumen, J.
Frère, Jean-Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Rossolini, G. M.
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Language :
English
Title :
Biochemical Characterization of the Fez-1 Metallo-Beta-Lactamase of Legionella Gormanii Atcc 33297t Produced in Escherichia Coli
Publication date :
April 2001
Journal title :
Antimicrobial Agents and Chemotherapy
ISSN :
0066-4804
eISSN :
1098-6596
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
45
Issue :
4
Pages :
1254-62
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2015

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