Poster (Scientific congresses and symposiums)
SPECIFICITY OF CLASS I TAGATOSE 1,6-BISPHOSPHATE ALDOLASE ENHANCED TOWARD TAGATOSE 1,6-BISPHOPHATE
Freichels, Régine; Delmarcelle, Michaël; Colarusso, Andrea et al.
201512th Biotrans
 

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Keywords :
tagatose 1,6-bisphosphate aldolase; specifitity
Abstract :
[en] Class I tagatose 1,6-bisphosphate aldolase catalyzes the reversible condensation of dihydroxyacetone phosphate and glyceraldehyde 3-phosphate to produce four D-ketohexoses 1,6-bisphosphate: D-tagatose 1,6-bisphosphate, D-fructose 1,6-bisphosphate, D-psicose 1,6-bisphosphate and D-sorbose 1,6-bisphosphate. These four sugars are diastereoisomers and differs from each other in their stereochemistry at carbons 3 and 4. The structure determination of three class I tagatose 1,6-bisphosphate aldolases has afforded new insight into their catalytic mechanism as well as their evolution. However, the determinant(s) that allow(s) the enzyme to be so unspecific at carbon 4 have remains unknown. The aim of this project is focused on the characterization of the structural features of tagatose 1,6-bisphosphate aldolases that determine the specificity of the enzyme towards tagatose versus fructose 1,6-bisphosphate (carbon C4).
Research center :
Center for Protein Engineering
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Freichels, Régine ;  Université de Liège > Département des sciences de la vie > Macromolécules biologiques
Delmarcelle, Michaël ;  Université de Liège > Centre d'ingénierie des protéines
Colarusso, Andrea
Kerff, Frédéric  ;  Université de Liège > Centre d'ingénierie des protéines
Wathelet, Bernard ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle
Galleni, Moreno ;  Université de Liège > Département des sciences de la vie > Macromolécules biologiques
Language :
English
Title :
SPECIFICITY OF CLASS I TAGATOSE 1,6-BISPHOSPHATE ALDOLASE ENHANCED TOWARD TAGATOSE 1,6-BISPHOPHATE
Publication date :
July 2015
Event name :
12th Biotrans
Event organizer :
Marko Mihovilovic - Vienna University of Technology
Event place :
Vienne, Austria
Event date :
du 26 juillet 2015 au 30 juillet 2015
Audience :
International
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
ULiège - Université de Liège [BE]
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since 04 September 2015

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