Reference : Phenotypic variability in bioprocessing conditions can be tracked on the basis of on-...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/184015
Phenotypic variability in bioprocessing conditions can be tracked on the basis of on-line flow cytometry and fits to a scaling law
English
Baert, Jonathan mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries >]
Kinet, Romain mailto [Université de Liège > > Centre Wallon de biologie industrielle >]
Brognaux, Alison mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries >]
Delepierre, Anissa mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries >]
Telek, Samuel mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries >]
Sorensen, Soren J. [> >]
Riber, Leise [> >]
Fickers, Patrick mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries >]
Delvigne, Frank mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries >]
2015
Biotechnology Journal
Yes (verified by ORBi)
International
1860-6768
1860-7314
[en] Noise in gene and protein expression is a major cause for bioprocess deviation. However, this phenomenon has been only scarcely considered in real bioprocessing conditions. In this work, a scaling-law derived from a genome-scale studies based on GFP reporter systems has been calibrated to an on-line flow cytometry device, allowing thus to get an insight at the level of promoter activity and associated noise during a whole microbial culture carried out in bioreactor. We show that most of the GFP reporter systems investigated and thus corresponding genes could be included inside the area covered by the scaling-law. The experimental results suggest that this scaling-law could be used to predict the dynamics of promoter activity, as well as the associated noise, in bioprocessing conditions. The knowledge acquired throughout this work could be used for the design of more robust expression systems.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Researchers ; Professionals
http://hdl.handle.net/2268/184015

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