Reference : Scalable temperature induced stress for the large-scale production of functionalized ...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/184014
Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria
English
Nguyen, Huu Thanh mailto [Université de Liège - ULiège > > > Form. doct. sc. agro. & ingé. biol.]
Razafindralambo, Hary [> >]
Richel, Aurore mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle >]
Jacquet, Nicolas mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle >]
Evrard, Pol [> >]
Antoine, Patrice [> >]
Thonart, Philippe mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries >]
Delvigne, Frank mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries >]
2015
Journal of Industrial Microbiology & Biotechnology
Springer
Yes (verified by ORBi)
International
1367-5435
1476-5535
Berlin
Germany
[en] The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of Bifidobacterium bifidum. However, this heat shock has been considered only in small scale bioreactor and no information is available for a possible scaling-up strategy. Five different operating scales (0.2 L, 2 L, 20 L, 200 L and 2000 L) have thus been tested and the results showed that the viability of B. bifidum increases from 3.15 to 6.57 folds, depending on the scale considered. Our observations pointed out the fact that the heat stress procedure is scalable according to the main outcome, i.e. increases in cell viability, but other factors have to be taken into account. Among these factors, dissolved carbon dioxide seems to play a significant role since it explain the differences observed between the test performed at lab-scale and in industrial conditions.
Researchers ; Professionals
http://hdl.handle.net/2268/184014

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