Determination of Zinc, Cadmium and Lead Bioavailability in Contaminated Soils at the Single-Cell Level by a Combination of Whole-Cell Biosensors and Flow Cytometry

Hurdebise, Quentin; Tarayre, Cédric; Fischer, Christophe; Colinet, Gilles; Hiligsmann, Serge; Delvigne, Frank

doi:10.3390/s150408981

Article (Scientific journals)

Determination of Zinc, Cadmium and Lead Bioavailability in Contaminated Soils at the Single-Cell Level by a Combination of Whole-Cell Biosensors and Flow Cytometry

Hurdebise, Quentin; Tarayre, Cédric; Fischer, Christophe et al.

2015 • In Sensors

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/181705

DOI
10.3390/s150408981

PubMed
25894939

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Keywords :

flow cytometry; Escherichia coli pPZntAgfp; cadmium; lead; zinc; bioavailability; whole-cell biosensors

Abstract :

[en] Zinc, lead and cadmium are metallic trace elements (MTEs) that are widespread in the environment and tend to accumulate in soils because of their low mobility and non-degradability. The purpose of this work is to evaluate the applicability of biosensors as tools able to provide data about the bioavailability of such MTEs in contaminated soils. Here, we tested the genetically-engineered strain Escherichia coli pPZntAgfp as a biosensor applicable to the detection of zinc, lead and cadmium by the biosynthesis of green fluorescent protein (GFP) accumulating inside the cells. Flow cytometry was used to investigate the fluorescence induced by the MTEs. A curvilinear response to zinc between 0 and 25 mg/L and another curvilinear response to cadmium between 0 and 1.5 mg/L were highlighted in liquid media, while lead did not produce exploitable results. The response relating to a Zn2+/Cd2+ ratio of 10 was further investigated. In these conditions, E. coli pPZntAgfp responded to cadmium only. Several contaminated soils with a Zn2+/Cd2+ ratio of 10 were analyzed with the biosensor, and the metallic concentrations were also measured by atomic absorption spectroscopy. Our results showed that E. coli pPZntAgfp could be used as a monitoring tool for contaminated soils being processed.

Disciplines :

Biotechnology

Author, co-author :

Hurdebise, Quentin ^✱; Université de Liège > Sciences et technologie de l'environnement > Physique des bio-systèmes

Tarayre, Cédric ^✱; Université de Liège > Chimie et bio-industries > Bio-industries

Fischer, Christophe ^✱; Université de Liège > Chimie et bio-industries > Bio-industries

Colinet, Gilles ; Université de Liège > Sciences et technologie de l'environnement > Systèmes Sol-Eau

Hiligsmann, Serge ; Université de Liège > Chimie et bio-industries > Bio-industries

Delvigne, Frank ; Université de Liège > Chimie et bio-industries > Bio-industries

^✱ These authors have contributed equally to this work.

Language :

English

Title :

Determination of Zinc, Cadmium and Lead Bioavailability in Contaminated Soils at the Single-Cell Level by a Combination of Whole-Cell Biosensors and Flow Cytometry

Publication date :

16 April 2015

Journal title :

Sensors

ISSN :

1424-8220

eISSN :

1424-3210

Publisher :

Molecular Diversity Preservation International (MDPI), Basel, Switzerland

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

BioRefine

Funders :

Programme Interreg IVB

Available on ORBi :

since 21 May 2015

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