[en] Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p'-DDT was the most potent xenoestrogen fromthe DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only 𝛽-HCH (at 400 and 2000 ng/mL) and 𝛿-HCH (at 2000 ng/mL) displayed estrogenic activities.These 13 organochlorines were determined by GC-MS in 12 samples of North Sea
harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 × 102∼4.2 × 104 𝜇g/kg), p,p'- DDE (5.1 × 102∼8.6 × 103 𝜇g/kg), and HCB (7.6 × 101∼1.5 × 103 𝜇g/kg) were the compounds found in highest concentrations.The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts.
Research Center/Unit :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Brose, François ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Département de sciences des denrées alimentaires (DDA)
Jauniaux, Thierry ; Université de Liège > Département de morphologie et pathologie > Département de morphologie et pathologie
Das, Krishna ; Université de Liège > Département de Biologie, Ecologie et Evolution > Océanologie
Muller, Marc ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Scippo, Marie-Louise ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires
Language :
English
Title :
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast
Hammond, P.S., Berggren, P., Benke, H., Abundance of harbour porpoise and other cetaceans in the North Sea and adjacent waters (2002) Journal of Applied Ecology, 39 (2), pp. 361-376
Beineke, A., Siebert, U., McLachlan, M., Investigations of the potential influence of environmental contaminants on the thymus and spleen of harbor porpoises (Phocoena phocoena) (2005) Environmental Science & Technology, 39 (11), pp. 3933-3938
Jauniaux, T., Petitjean, D., Brenez, C., Post-mortem findings and causes of death of harbour porpoises (Phocoena phocoena) stranded from 1990 to 2000 along the coastlines of Belgiumand Northern France (2002) Journal of Comparative Pathology, 126 (4), pp. 243-253
Peltier, H., Baagøe, H.J., Camphuysen, K.C.J., The stranding anomaly as population indicator: The case of harbour porpoise Phocoena phocoena in north-western europe (2013) PLoS ONE, 8 (4)
Mössner, S., Ballschmiter, K., Marine mammals as global pollution indicators for organochlorines (1997) Chemosphere, 34 (5-7), pp. 1285-1296
Law, R.J., Bersuder, P., Barry, J., Deaville, R., Reid, R.J., Jepson, P.D., Chlorobiphenyls in the blubber of harbour porpoises (Phocoena phocoena) fromtheUK: Levels andtrends 1991-2005 (2010) Marine Pollution Bulletin, 60 (3), pp. 470-473
Pierce, G.J., Santos, M.B., Murphy, S., Bioaccumulation of persistent organic pollutants in female common dolphins (Delphinus delphis) and harbour porpoises (Phocoena phocoena) fromwestern European seas: Geographical trends, causal factors and effects on reproduction and mortality (2008) Environmental Pollution, 153 (2), pp. 401-415
Tanabe, S., Contamination and toxic effects of persistent endocrine disrupters in marine mammals and birds (2002) Marine Pollution Bulletin, 45 (1-12), pp. 69-77
Tanabe, S., Sung, J.-K., Choi, D.-Y., Persistent organochlorine residues in northern fur seal fromthe Pacific coast of Japan since 1971 (1994) Environmental Pollution, 85 (3), pp. 305-314
Bruhn, R., Kannan, N., Petrick, G., Schulz-Bull, D.E., Duinker, J.C., CB pattern in the harbour porpoise: Bioaccumulation, metabolism and evidence for cytochrome p450 IIB activity (1995) Chemosphere, 31 (7), pp. 3721-3732
Boon, J.P., Meer Der J.Van, Allchin, C.R., Concentrationdependent changes of PCB patterns in fish-eating mammals: Structural evidence for induction of cytochrome P450 (1997) Archives of Environmental Contamination and Toxicology, 33 (3), pp. 298-311
Alegrá-Torres, J.A., Dáz-Barriga, F., Gandolfi, A.J., Pérez-Maldonado, I.N., Mechanisms of,-DDE-induced apoptosis in human peripheral blood mononuclear cells (2009) Toxicology in Vitro, 23 (6), pp. 1000-1006
Abhilash, P.C., Singh, N., Distribution of hexachlorocyclohexane isomers in soil samples from a small scale industrial area of Lucknow, North India, associated with lindane production (2008) Chemosphere, 73 (6), pp. 1011-1015
Covaci, A., Vijver De K.Van, Decoen, W., Determination of organohalogenated contaminants in liver of harbour porpoises (Phocoena phocoena) stranded on the Belgian North Sea coast (2002) Marine Pollution Bulletin, 44 (10), pp. 1157-1165
Safe, S.H., Endocrine disruptors and human health-is there a problem An update (2000) Environmental Health Perspectives, 108 (6), pp. 487-493
Munoz-De-Toro, M., Beldoménico, H.R., Garcá, S.R., Organochlorine levels in adipose tissue of women from a littoral region of Argentina (2006) Environmental Research, 102 (1), pp. 107-112
Porte, C., Janer, G., Lorusso, L.C., Endocrine disruptors in marine organisms: Approaches and perspectives (2006) Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 143 (3), pp. 303-315
Bila, D.M., Dezotti, M., Endocrine disrupters in the environment: Part 1-effects and consequences (2007) Qúmica Nova, 30 (3), pp. 651-666
Ross, P.S., Couillard, C.M., Ikonomou, M.G., Large and growing environmental reservoirs of Deca-BDE present an emerging health risk for fish and marine mammals (2009) Marine Pollution Bulletin, 58 (1), pp. 7-10
Sonne, C., Letcher, R.J., Bechshøft, T.O., Two decades of biomonitoring polar bear health in Greenland: A review (2012) Acta Veterinaria Scandinavica, 54, p. S15
Smith, A.G., Gangolli, S.D., Organochlorine chemicals in seafood: Occurrence and health concerns (2002) Food and Chemical Toxicology, 40 (6), pp. 767-779
Willemsen, P., Scippo, M.-L., Kausel, G., Use of reporter cell lines for detection of endocrine-disrupter activity (2004) Analytical and Bioanalytical Chemistry, 378 (3), pp. 655-663
Maranghi, F., Rescia, M., Macr, C., Lindanemay modulate the female reproductive development through the interaction with ER-: An in vivo-in vitro approach (2007) Chemico-Biological Interactions, 169 (1), pp. 1-14
Jepson, P.D., Bennett, P.M., Deaville, R., Allchin, C.R., Baker, J.R., Law, A.J., Relationships betweenpolychlorinatedbiphenyls and health status in harbor porpoises (Phocoena phocoena) stranded in the United Kingdom (2005) Environmental Toxicology and Chemistry, 24 (1), pp. 238-248
Hall, A.J., Hugunin, K., Deaville, R., Law, R.J., Allchin, C.R., Jepson, P.D., The risk of infection from polychlorinated biphenyl exposure in the harbor porpoise (Phocoena phocoena): A casecontrol approach (2006) Environmental Health Perspectives, 114 (5), pp. 704-711
Das, K., Vossen, A., Tolley, K., Interfollicular fibrosis in the thyroid of the harbour porpoise: An endocrine disruption (2006) Archives of Environmental Contamination and Toxicology, 51 (4), pp. 720-729
Garritano, S., Pinto, B., Calderisi, M., Cirillo, T., Amodio-Cocchieri, R., Reali, D., Estrogen-like activity of seafood related to environmental chemical contaminants (2006) Environmental Health:AGlobal Access Science Source, 5
Guéguen, M., Amiard, J.-C., Arnich, N., Shellfish and residual chemical contaminants: Hazards, monitoring, and health risk assessment along French coasts (2011) Reviews of Environmental Contamination and Toxicology, 213, pp. 55-111
Scippo, M.-L., Maghuin-Rogister, G., Endocrine disruptors in food: Potential impact on human health (2007) Annales de Medecine Veterinaire, 151 (1), pp. 44-54
Nadzialek, S., Vanparys, C., Heiden Der E.Van, Understanding the gap between the estrogenicity of an effluent and its real impact into the wild (2010) Science of the Total Environment, 408 (4), pp. 812-821
Willemsen, P., Scippo, M.-L., Maghuin-Rogister, G., Martial, J.A., Muller, M., Use of specific bioluminescent cell lines for the detection of steroid hormone (ant)agonists in meat producing animals (2002) Analytica Chimica Acta, 473 (1-2), pp. 119-126
Streck, G., Chemical and biological analysis of estrogenic, progestagenic and androgenic steroids in the environment (2009) Trends in Analytical Chemistry, 28 (6), pp. 635-652
Houtman, C.J., Booij, P., Jover, E., Estrogenic and dioxin-like compounds in sediment from Zierikzee harbour identified with CALUX assay-directed fractionation combined with one and two dimensional gas chromatography analyses (2006) Chemosphere, 65 (11), pp. 2244-2252
Cai, K., Elliott, C.T., Phillips, D.H., Scippo, M.-L., Muller, M., Connolly, L., Treatment of estrogens and androgens in dairy wastewater by a constructed wetland system (2012) Water Research, 46 (7), pp. 2333-2343
Houtman, C.J., Van Oostveen, A.M., Brouwer, A., Lamoree, M.H., Legler, J., Identification of estrogenic compounds in fish bile using bioassay-directed fractionation (2004) Environmental Science & Technology, 38 (23), pp. 6415-6423
Simon, E., Lamoree, M.H., Hamers, T., Testing endocrine disruption in biota samples: A method to remove interfering lipids and natural hormones (2010) Environmental Science and Technology, 44 (21), pp. 8322-8329
Suzuki, G., Tue, N.M., Linden Der S.Van, Identification of major dioxin-like compounds and androgen receptor antagonist in acid-treated tissue extracts of high trophic-level animals (2011) Environmental Science & Technology, 45 (23), pp. 10203-10211
Willemsen, P., Scippo, M.-L., Maghuin-Rogister, G., Martial, J.A., Muller, M., Enhancement of steroid receptor-mediated transcription for the development of highly responsive bioassays (2005) Analytical and Bioanalytical Chemistry, 382 (4), pp. 894-905
Jayle, M.-F., Crépy, O., Hydrolyse, extraction et purification des stérodes (1961) Analyses des StérodesHormonaux: Tome i, pp. 61-113. , M.-F. Jayle, Ed., Masson et Cie Editeurs, Paris, France
Rivas, A., Olea, N., Olea-Serrano, F., Human exposure to endocrine-disrupting chemicals: Assessing the total estrogenic xenobiotic burden (1997) TrAC: Trends in Analytical Chemistry, 16 (10), pp. 613-619
Sebaugh, J.L., McCray, P.D., Defining the linear portion of a sigmoid-shaped curve: Bend points (2003) Pharmaceutical Statistics, 2 (3), pp. 167-174
Payne, J., Jones, C., Lakhani, S., Kortenkamp, A., Improving the reproducibility of theMCF-7 cell proliferation assay for the detection of xenoestrogens (2000) Science of the Total Environment, 248 (1), pp. 51-62
Hatakeyama, M., Tessier, D.M., Dunlap, D.Y., Zou, E., Matsumura, F., Estrogenic action of-HCH through activation of c-Neu in MCF-7 breast carcinoma cells (2002) Environmental Toxicology and Pharmacology, 11 (1), pp. 27-38
Hatakeyama, M., Zou, E., Matsumura, F., Comparison of the characteristic of estrogenic action patterns of-HCH and heregulin 1 in MCF-7 human breast cancer cells (2002) Journal of Biochemical and Molecular Toxicology, 16 (5), pp. 209-219
Marabini, L., Chiesara, E., Radice, S., Possible estrogenic effects of some highly bioaccumulated polychlorinated biphenyls (PCB 101, 118,138,153) alone and in mixture in MCF-7 breast cancer cells (2007) Toxicology Letters, 172, pp. S49-S50
Pl Sková, M., Vondrácek, J., Canton, R.F., Impact of polychlorinated biphenyls contamination on estrogenic activity in human male serum (2005) Environmental Health Perspectives, 113 (10), pp. 1277-1284
Hamers, T., Kamstra, J.H., Cenijn, P.H., In vitro toxicity profiling of ultrapure non-dioxin-like polychlorinated biphenyl congeners and their relative toxic contribution to PCB mixtures in humans (2011) Toxicological Sciences, 121 (1), pp. 88-100
Payne, J., Rajapakse, N., Wilkins, M., Kortenkamp, A., Prediction and assessment of the effects of mixtures of four xenoestrogens (2000) Environmental Health Perspectives, 108 (10), pp. 983-987
Daston, G.P., Cook, J.C., Kavlock, R.J., Uncertainties for endocrine disrupters: Our view on progress (2003) Toxicological Sciences, 74 (2), pp. 245-252
Tinwell, H., Ashby, J., Sensitivity of the immature rat uterotrophic assay to mixtures of estrogens (2004) Environmental Health Perspectives, 112 (5), pp. 575-582
Vanmeeuwen, J.A., Berg Den M.Van, Sanderson, J.T., Verhoef, A., Piersma, A.H., Estrogenic effects of mixtures of phytoand synthetic chemicals on uterine growth of prepubertal rats (2007) Toxicology Letters, 170 (2), pp. 165-176
Habibi, H., Jeffries, K., Nelson, E., Jackson, L., Risk assessment for endocrine disrupting chemical mixtures (2008) Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 148 (4), p. 455
Weijs, L., Van Elk, C., Das, K., Blust, R., Covaci, A., Persistent organic pollutants and methoxylated PBDEs in harbour porpoises from the North Sea from 1990 until 2008: Young wildlife at risk (2010) Science of the Total Environment, 409 (1), pp. 228-237
Law, R.J., Bolam, T., James, D., Butyltin compounds in liver of harbour porpoises (Phocoena phocoena) from the UK prior to and following the ban on the use of tributyltin in antifouling paints (1992-2005 & 2009) (2012) Marine Pollution Bulletin, 64 (11), pp. 2576-2580
Weijs, L., Das, K., Siebert, U., Concentrations of chlorinated and brominated contaminants and their metabolites in serum of harbour seals and harbour porpoises (2009) Environment International, 35 (6), pp. 842-850
Weijs, L., Dirtu, A.C., Das, K., Inter-species differences for polychlorinated biphenyls and polybrominated diphenyl ethers in marine top predators from the Southern North Sea. Part 1. Accumulation patterns in harbour seals and harbour porpoises (2009) Environmental Pollution, 157 (2), pp. 437-444
Mahfouz, C., Henry, F., Jauniaux, T., Khalaf, G., Amara, R., Organochlorines in harbour porpoises (Phocoena phocoena) stranded along the southern North Sea between 2010 and 2013 (2014) Nvironmental Science: Processes & Impacts, 16 (12), pp. 2774-2781
Kwong, R.W.M., Yu, P.K.N., Lam, P.K.S., Wang, W.-X., Uptake, elimination, and biotransformation of aqueous and dietary DDT in marine fish (2008) Environmental Toxicology and Chemistry, 27 (10), pp. 2053-2063
Kwong, R.W.M., Yu, P.K.N., Lam, P.K.S., Wang, W.-X., Biokinetics and biotransformation of DDTs in the marine green mussels Perna viridis (2009) Aquatic Toxicology, 93 (4), pp. 196-204
Benezet, H.J., Matsumura, F., Isomerization of-BHC to-BHC in the environment (1973) Nature, 243 (5407), pp. 480-481
Voorspoels, S., Covaci, A., Maervoet, J., De Meester, I., Schepens, P., Levels and profiles of PCBs and OCPs in marine benthic species from the Belgian North Sea and the Western Scheldt Estuary (2004) Marine Pollution Bulletin, 49 (5-6), pp. 393-404
Barrie, L.A., Gregor, D., Hargrave, B., Arctic contaminants: Sources, occurrence and pathways (1992) Science of the Total Environment, 122 (1-2), pp. 1-74
Saadati, N., Abdullah, M.P., Zakaria, Z., Rezayi, M., Hosseinizare, N., Distribution and fate of HCH isomers and DDT metabolites in a tropical environment-case study Cameron Highlands-Malaysia (2012) Chemistry Central Journal, 6 (1)
Kleivane, L., Skaare, J.U., Bjørge, A., De Ruiter, E., Reijnders, P.J.H., Organochlorine pesticide residue and PCBs in harbour porpoise (Phocoena phocoena) incidentally caught in Scandinavianwaters (1995) Environmental Pollution, 89 (2), pp. 137-146
Krahn, M.M., Hanson, M.B., Schorr, G.S., Effects of age, sex and reproductive status on persistent organic pollutant concentrations in Southern Resident' killer whales (2009) Marine Pollution Bulletin, 58, pp. 1522-1529
Navas, J.M., Segner, H., Antiestrogenic activity of anthropogenic and natural chemicals (1998) Environmental Science and Pollution Research International, 5 (2), pp. 75-82
