Metagenomic analysis of the bacterial microbiota linked to the traditional Algerian date product "Btana"

Abekhti, Abdelkader; Taminiau, Bernard; Kihal, Mabrouk; Daube, Georges

doi:10.1007/s13213-015-1084-3

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Metagenomic analysis of the bacterial microbiota linked to the traditional Algerian date product "Btana"

Abekhti, Abdelkader; Taminiau, Bernard; Kihal, Mabrouk et al.

2015 • In Annals of Microbiology

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/180864

DOI
10.1007/s13213-015-1084-3

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Keywords :

Pyrosequencing; Bacterial diversity; Traditional food; Dates; Phoenix dactylifera L; Btana

Abstract :

[en] In this study, using high throughput pyrosequencing, we highlighted the bacterial diversity of the traditional Algerian date product "Btana" that is produced in southern Algeria using both direct (DBM) and indirect (UBM) methods. Metagenomic analysis yielded a total of 103,379 reads, with a 606 total operational taxonomic units (OTUs) detected. Firmicutes represented 84.79 % of the total pyrosequencing reads. Phylogenetic analysis revealed that the Bacillales represented 90.20 % ± 15.12 % of the total reads. Among the phylotypes detected, Bacillus was the dominant genus (39.53 %). While Bacillus megaterium was shared among all of the samples, its distribution varied widely. Non-metric multidimensional scaling (nMDS) analysis showed that UBM samples clustered together, and three main OTUs were found in these UBM samples: Paenibacillus polymyxa, Paenibacillus xylanexedens, and Planomicrobium JN082684. Correlation analysis showed no association between parametersof the samples (age, pH, water activity) and the specific microbiota.

Research Center/Unit :

Fundamental & Applied Research for Animal and Health (FARAH)

Disciplines :

Microbiology

Author, co-author :

Abekhti, Abdelkader; Université de Biskra, Algérie > Natural and Life Sciences > Exact Sciences and Natural and Life Sciences,

Taminiau, Bernard ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Kihal, Mabrouk; Université d'Oran, Algérie > Biology > Applied Microbiology

Daube, Georges ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Language :

English

Title :

Metagenomic analysis of the bacterial microbiota linked to the traditional Algerian date product "Btana"

Publication date :

2015

Journal title :

Annals of Microbiology

ISSN :

1590-4261

eISSN :

1869-2044

Publisher :

Springer, Germany

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 April 2015

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