Article (Scientific journals)
Impact of the Structure of Biocompatible Aliphatic Polycarbonates on siRNA Transfection Ability
Frère, Antoine; Kawalec, Michal; Tempelaar, Sarah et al.
2015In Biomacromolecules
Peer Reviewed verified by ORBi
 

Files


Full Text
Impact of the Structure of Biocompatible Aliphatic Polycarbonates on siRNA Transfection Ability.pdf
Author postprint (1.75 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
ring-opening polymerization; aliphatic polycarbonates; guanidinium; morpholine; polyplex; siRNA; HDAC
Abstract :
[en] RNAi therapeutics are promising therapeutic tools that have sparked the interest of many researchers. In an effort to provide a safe alternative to PEI, we have designed a series of new guanidinium and/or morpholino functionalized biocompatible and biodegradable polycarbonate vectors. The impact of different functions (morpholino-, guanidinium-, hydrophobic groups), of the architecture (linear homopolymer to dumbbell-shape) and of the molecular weight of these copolymers on their capacity to form polyplexes and to decrease the expression of two epigenetic regulators of gene expression, HDAC7 and HDAC5 was evaluated. The use of one of these polymers combining morpholine and guanidine functions at the ratio >1 and hydrophobic trimethylene carbonate groups showed a significant decrease of mRNA and protein level in HeLa cells, similar to PEI. These results highlight the potential of polycarbonate vectors for future in vivo application as an anti-cancer therapy.
Research center :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Frère, Antoine ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Kawalec, Michal;  Université de Mons - UMONS
Tempelaar, Sarah;  Université de Mons - UMONS
Peixoto, Paul ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases
Hendrick, Elodie ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et biologie moléculaires animales
Peulen, Olivier  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Evrard, Brigitte  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Dubois, Philippe;  Université de Mons - UMONS
Mespouille, Laetitia;  Université de Mons - UMONS
Mottet, Denis ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et biologie moléculaires animales
Piel, Géraldine ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Language :
English
Title :
Impact of the Structure of Biocompatible Aliphatic Polycarbonates on siRNA Transfection Ability
Publication date :
January 2015
Journal title :
Biomacromolecules
ISSN :
1525-7797
eISSN :
1526-4602
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 30 January 2015

Statistics


Number of views
139 (32 by ULiège)
Number of downloads
4 (4 by ULiège)

Scopus citations®
 
23
Scopus citations®
without self-citations
18
OpenCitations
 
24

Bibliography


Similar publications



Contact ORBi