Article (Scientific journals)
Crystal structure of penicillin-binding protein 3 (PBP3) from Escherichia coli
Sauvage, Eric; Derouaux, Adeline; Fraipont, Claudine et al.
2014In PLoS ONE
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Abstract :
[en] In Escherichia coli, penicillin-binding protein 3 (PBP3), also known as FtsI, is a central component of the divisome, catalyzing cross-linking of the cell wall peptidoglycan during cell division. PBP3 is mainly periplasmic, with a 23 residues cytoplasmic tail and a single transmembrane helix. We have solved the crystal structure of a soluble form of PBP3 (PBP357-577) at 2.5 Å revealing the two modules of high molecular weight class B PBPs, a carboxy terminal module exhibiting transpeptidase activity and an amino terminal module with unknown function. To gain additional insight, the PBP3 Val88-Ser165 subdomain (PBP388-165), for which the electron density is poorly defined in the PBP3 crystal, was produced and its structure solved by SAD phasing at 2.1 Å. The structure shows a three dimensional domain swapping with a β-strand of one molecule inserted between two strands of the paired molecule, suggesting a possible role in PBP357-577 dimerization.
Research center :
d‐BRU - Dental Biomaterials Research Unit - ULiège [BE]
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sauvage, Eric ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Derouaux, Adeline ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Fraipont, Claudine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Joris, Marine
Herman, Raphaël ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Rocaboy, Mathieu
Schloesser, Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Dumas, Jacques
Kerff, Frédéric  ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Charlier, Paulette ;  Université de Liège - ULiège > Département des sciences de la vie > Cristallographie des macromolécules biologiques
Language :
English
Title :
Crystal structure of penicillin-binding protein 3 (PBP3) from Escherichia coli
Publication date :
2014
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 June 2014

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