Stochastic exposure to sub-lethal high temperature enhances exopolysaccharides (EPS) excretion and improves Bifidobacterium bifidum cell survival to freeze-drying

Nguyen, Huu Thanh; Razafindralambo, Hary; Blecker, Christophe; N'Yapo, Charles; Thonart, Philippe; Delvigne, Frank

doi:10.1016/j.bej.2014.04.005

Article (Scientific journals)

Stochastic exposure to sub-lethal high temperature enhances exopolysaccharides (EPS) excretion and improves Bifidobacterium bifidum cell survival to freeze-drying

Nguyen, Huu Thanh; Razafindralambo, Hary; Blecker, Christophe et al.

2014 • In Biochemical Engineering Journal, 88, p. 85-94

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/166756

DOI
10.1016/j.bej.2014.04.005

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Keywords :

Bifidobacteria,; Exopolysaccharide, Bioreactors,; Microbial stress, Viability

Abstract :

[en] Exposure of microbial cells to sub-lethal stresses is known to increase cell robustness. In this work, a two-compartment bioreactor in which microbial cells are stochastically exposed to sub-lethal temperature stresses has been used in order to investigate the response of the stress sensitive Bifidobacterium bifidum THT 0101 to downstream processing operations. A stochastic model validated by residence time distribution experiments has shown that in the heat-shock configuration, a two-compartment bioreactor (TCB) allows the exposure of microbial cells to sub-lethal temperature of 42°C for a duration comprised between 100 and 300 seconds. This exposure resulted in a significant increase of cell resistance to freeze-drying by comparison with cells cultivated in conventional bioreactors or in the TCB in the cold shock mode (CS-TCB). The mechanism behind this robustness seems to be related with the coating of microbial cells with exopolysaccharide (EPS), as assessed by the change of the zeta potential and the presence of higher EPS concentration after heat shock. Conditioning of Bifidobacteria on the basis of the heat shock technique is interesting from the practical and economical point of view since this strategy can be directly implemented in the bioreactor during stationary phase preceding cell recovery and freeze-drying

Disciplines :

Biotechnology

Author, co-author :

Nguyen, Huu Thanh ; Université de Liège - ULiège > Form. doct. sc. agro. & ingé. biol.

Razafindralambo, Hary

Blecker, Christophe ; Université de Liège - ULiège > Chimie et bio-industries > Science des alim. et formul.

N'Yapo, Charles

Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles

Delvigne, Frank ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries

Language :

English

Title :

Stochastic exposure to sub-lethal high temperature enhances exopolysaccharides (EPS) excretion and improves Bifidobacterium bifidum cell survival to freeze-drying

Publication date :

24 April 2014

Journal title :

Biochemical Engineering Journal

ISSN :

1369-703X

Publisher :

Elsevier Science, Lausanne, Switzerland

Volume :

Pages :

85-94

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://dx.doi.org/10.1016/j.bej.2014.04.005

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 05 May 2014

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