Article (Scientific journals)
Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses.
Simonis, Nicolas; Rual, Jean-Francois; Lemmens, Irma et al.
2012In Retrovirology, 9, p. 26
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Keywords :
Host-Pathogen Interactions; Human T-lymphotropic virus 1/immunology/pathogenicity; Human T-lymphotropic virus 2/immunology/pathogenicity; Humans; Systems Biology/methods; T-Lymphocytes/immunology/virology; Two-Hybrid System Techniques
Abstract :
[en] BACKGROUND: Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression. RESULTS: We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway. CONCLUSIONS: This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and -2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Simonis, Nicolas
Rual, Jean-Francois
Lemmens, Irma
Boxus, Mathieu
Hirozane-Kishikawa, Tomoko
GATOT, Jean-Stéphane 
Dricot, Amelie
Hao, Tong
Vertommen, Didier
Legros, Sebastien
Daakour, Sarah ;  Université de Liège > GIGA-Research
Klitgord, Niels
Martin, Maud ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
Willaert, Jean-Francois
Dequiedt, Franck  ;  Université de Liège - ULiège > GIGA-Research
Navratil, Vincent
Cusick, Michael E.
Burny, Arsène ;  Université de Liège - ULiège > Agro Biotech Gembloux
Van Lint, Carine
Hill, David E.
Tavernier, Jan
Kettmann, Richard ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
Vidal, Marc
Twizere, Jean-Claude  ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
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Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses.
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