Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses.

Host-Pathogen Interactions; Human T-lymphotropic virus 1/immunology/pathogenicity; Human T-lymphotropic virus 2/immunology/pathogenicity; Humans; Systems Biology/methods; T-Lymphocytes/immunology/virology; Two-Hybrid System Techniques

Abstract :

[en] BACKGROUND: Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression. RESULTS: We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway. CONCLUSIONS: This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and -2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Simonis, Nicolas

Rual, Jean-Francois

Lemmens, Irma

Boxus, Mathieu

Hirozane-Kishikawa, Tomoko

GATOT, Jean-Stéphane

Dricot, Amelie

Hao, Tong

Vertommen, Didier

Legros, Sebastien

More authors (14 more)

Language :

English

Title :

Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses.

Publication date :

2012

Journal title :

Retrovirology

eISSN :

1742-4690

Publisher :

Springer

Volume :

Pages :

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.ncbi.nlm.nih.gov/pubmed/22458338

Available on ORBi :

since 11 April 2014

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