Direct determination of tagitinin C in Tithonia diversifolia leaves by on-line coupling of supercritical carbon dioxide extraction to FT-IR spectroscopy by means of optical fibres
[en] Supercritical fluid extraction (SFE) with carbon dioxide as extraction medium was on-line coupled to a FT-IR spectrometer equipped with a Mercury Cadmium Telluride (MCT) detector using a tailor-made high-pressure fibre optic flow cell. This method was optimised and developed for the monitoring in real time and the quantification of dynamic extractions of tagitinin C from Tithonia.diversifolia leaves. In order to demonstrate the method ability to allow the direct quantification of tagitinin C in the extract medium the standard addition method was used. The area integration Of Curves obtained by plotting the absorbance of the highly specific C=O stretching vibration at 1668 cm(-1) versus time (i.e. extractograms) was used as instrumental response. The SFE/FT-IR process was successfully validated using the accuracy profile as decision tool. On this basis, a linear regression model was chosen for the calibration curve. The relative standard deviation for repeatability and intermediate precision were between 0.8 and 3.1 %, respectively. Moreover, the method was found to be accurate as the two-sided 95% beta-expectation tolerance interval did not exceed the acceptance limits of 85 and 115% on the analytical range investigated (500-2500 mu g of added amount of tagitinin Q. The proposed method allowed the non-destructive extraction of tagitinin C and its on-line quantitative determination in less than 25 min thus facilitating the subsequent experiments or the pharmacological studies performed on this compound.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Ziemons, Eric ; Université de Liège - ULiège > Chimie analytique
Barillaro, Valéry
Rozet, Eric ; Université de Liège - ULiège > Chimie analytique
Mbakop, N. W.
Lejeune, Robert ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Thunus, Léopold ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Direct determination of tagitinin C in Tithonia diversifolia leaves by on-line coupling of supercritical carbon dioxide extraction to FT-IR spectroscopy by means of optical fibres
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Goffin E., Ziemons E., De Mol P.M., de Madureira M., Martins A.P., Proença da Cunha A., Philippe G., Tits M., Angenot L., and Frederich M. Planta Med. 68 (2002) 543
Gu J., Gills J., Park E.J., Mata-Greenwood E., Hawthorne M., Axelrod F., Chavez P., Fong H., Mehta R., Pezzuto J., and Kinghorn A. J. Nat. Prod. 65 (2002) 532
Benner B., Goodpaster J., Degrasse J., Tully L., and Levin B. J. Forensic Sci. 48 (2003) 554
Stone M., and Taylor L. J. Chromatogr. A 931 (2001) 53
Batlle R., Carlsson H., Holmgren E., Colmsjö A., and Crescenzi C. J. Chromatogr. A 963 (2002) 73
Wang Z., Ashraf-Khorassani M., and Taylor L. J. Chromatogr. A 1033 (2004) 221
Pòl J., Hyötyläinen T., Ranta-Aho O., and Riekkola M.-L. J. Chromatogr. A 1052 (2004) 25
Zougagh M., Redigolo H., Rìos A., and Valcàrcel M. Anal. Chim. Acta 525 (2004) 265
Tena M., and Valcàrcel M. J. Chromatogr. A 753 (1996) 299
Tena M., Luque de Castro M., and Valcàrcel M. Anal. Chem. 68 (1996) 2386
Wang J., and Mashall W. Anal. Chem. 66 (1994) 3900
Braumann U., Händel H., and Albert K. Anal. Chem. 67 (1995) 930
Braumann U., Händel H., Strohschein S., Spraul M., Krack G., Ecker R., and Albert K. J. Chromatogr. A 761 (1997) 336
Kalinoski H., Udseth H., Wright B., and Simth R. Anal. Chem. 58 (1986) 2421
Jublot L., Linforth R., and Taylor A. J. Chromatogr. A 1056 (2004) 27
Heglund D., Tilotta D., Hawthorne S., and Miller D. Anal. Chem. 66 (1994) 3543
Kirschner C., Jordan S., and Taylor L. Anal. Chem. 66 (1994) 882
Current R., and Tilotta D. J. Chromatogr. A 785 (1997) 269
Liang S., and Tilotta D. J. Chromatogr. A 986 (2003) 319
Ziemons E., Goffin E., Lejeune R., Angenot L., and Thunus L. Talanta 62 (2004) 383
Ziemons E., Goffin E., Lejeune R., Proença da Cunha A., Angenot L., and Thunus L. J. Supercrit. Fluids 33 (2005) 53
Hubert Ph., Nguyen-Huu J.-J., Boulanger B., Chapuzet E., Chiap P., Cohen N., Compagnon P.-A., Dewé W., Feinberg M., Lallier M., Laurentie M., Mercier N., Muzard G., Nivet C., and Valat L. J. Pharm. Biomed. Anal. 36 (2004) 579
Guidance for Industry: Bioanalytical Method Valiadation, U.S. Departement of Health and Human Services, Food and Drug Administration, Center of Drug Evaluation and Research, Center for Biologics Evaluation and Research, May 2001.
Miller J.C., and Miller J.N. Statistics for Analytical Chemistry. fifth ed. (2000), Ellis Horwood, New York pp. 96-100
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.