Antioxidant capacity of hydrophilic food matrices: optimization and validation of ORAC assay.

[en] It is widely accepted that ORAC is a useful method for assessing food extracts that contain various antioxidants. The principal aim of this study was to validate the ORAC assay. We first identified parameters that can interfere with the ORAC assay and we optimized it. Then, experiments were conducted to determine the limits of linearity and response function, to determine the accuracy profiles to circumvent some of the drawbacks of traditional validation procedures. Trueness, selectivity and limits of quantification of the method were also determined. Our objective of ORAC method validation is thus to give guarantees that most of the results generated during use of this method will be close enough to unknown true value of antioxidant capacity of food matrices. The validation results indicate that the described method will give accurate and reliable results for Trolox equivalent values ranging from 50 to 200 µmol/L.

Disciplines :

Food science

Author, co-author :

Kevers, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie

Sipel, Arnaud; Université de Liège - ULiège > Sciences de la vie > Biologie moléculaire et Biotechnologie Végétales

PINCEMAIL, Joël ; Centre Hospitalier Universitaire de Liège - CHU > Chirurgie cardio-vasculaire

Dommes, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales

Language :

English

Title :

Antioxidant capacity of hydrophilic food matrices: optimization and validation of ORAC assay.

Publication date :

2014

Journal title :

Food Analytical Methods

ISSN :

1936-9751

eISSN :

1936-976X

Publisher :

Springer, New York, United States - New York

Volume :

Pages :

409-416

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 10 June 2013

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Bibliography

Augustin W, Wiswedel I, Noack H, Reinheckel T, Reichelt O (1997) Mol Cell Biochem 174: 199.
Cao G, Prior RL (1998) Clin Chem 44: 1309.
Cavalier E, Rozet E, Dubois N, Charlier C, Hubert P, Chapelle JP, Krzesinski JM, Delanaye P (2008) Clin Chim Acta 396: 80.
Cook A, Le A (2006) J Phys Chem Lab 10: 44.
Ferreira I, Baptista P, Vilas-Boas M, Barros L (2007) Food Chem 100: 1511.
Halliwell B (2009) Free Radic Biol Med 46: 531.
Hubert P, Nguyen-Huu J, Boulanger B, Chapuzet E, Chiap P, Cohen N, Compagnon P, Dewe W, Feinberg M, Lallier M, Laurentie M, Mercier N, Muzard G, Nivet C, Valat L (2003) Validation des procédures analytiques quantitatives harmonisation des démarches 13: 101.
Hubert P, Nguyen-Huu J, Boulanger B, Chapuzet E, Chiap P, Cohen N, Compagnon P, Dewé W, Feinberg M, Lallier M, Laurentie M, Mercier N, Muzard G, Nivet C, Valat L (2004) J Pharm Biomed Anal 36: 579.
Hubert P, Nguyen-Huu J, Boulanger B, Chapuzet E, Cohen N, Compagnon P, Dewé W, Feinberg M, Laurentie M, Mercier N, Muzard G, Valat L, Rozet E (2007) J Pharm Biomed Anal 45: 82.
Klonis N, Sawyer W (2000) Photochem Photobiol 72: 179.
Mikami I, Yamaguchi M, Shinmoto H, Tsushida T (2009) Food Sci Technol Res 15: 171.
Ou B, Hampsch-Woodill M, Prior R (2001) J Agric Food Chem 49: 4619.
Pérez-Jiménez J, Saura-Calixto F (2006) Food Res Int 39: 791.
Pérez-Jiménez J, Arranz S, Tabernero M, Díaz-Rubio M, Serrano J, Goñi I, Saura-Calixto F (2008) Food Res Int 41: 274.
Price J, Sanny C, Shevlin D (2006) J Pharmacol Toxicol Methods 54: 56.
Prior R, Hoang H, Gu L, Wu X, Bacchiocca M, Howard L, Hampsch-Woodill M, Huang D, Ou B, Jacob RR (2003) J Agric Food Chem 51: 3273.
Prior R, Wu X, Schaick K (2005) J Agric Food Chem 53: 4290.
Rozet E, Ceccato A, Hubert C, Ziemons E, Oprean R, Rudaz S, Boulanger B, Hubert P (2007) J Chromatogr A 1158: 111.
Sipel A, Kevers C, Pincemail J, Dommes J (2013) Food Analytical Methods In press.
Tabart J, Kevers C, Sipel A, Pincemail J, Defraigne J, Dommes J (2007) Food Chem 105: 1268.
Tabart J, Franck T, Kevers C, Pincemail J, Serteyn D, Defraigne J, Dommes J (2012) Food Chem 131: 1116.
Tucker G, Robards K (2008) Crit Rev Food Sci Nutr 48: 929.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Int J Biochem Cell Biol 39: 44.
Watanabe J, Oki T, Takebayashi J, Yamasaki K, Takano-Ishikawa Y, Hino A, Yasui A (2012) Anal Sci 28: 159.
Wu X, Beecher G, Holden J, Haytowitz D, Gebhardt S, Prior R (2004) J Agric Food Chem 52: 4026.

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