[en] A carbapenem-resistant Pseudomonas aeruginosa strain (PA41437) susceptible to expanded-spectrum cephalosporins was recovered from several consecutive lower-respiratory-tract specimens of a patient who developed a ventilator-associated pneumonia while hospitalized in an intensive care unit. Cloning experiments identified OXA-198, a new class D β-lactamase which was weakly related (less than 45% amino acid identity) to other class D β-lactamases. Expression in Escherichia coli TOP10 and in P. aeruginosa PAO1 led to transformants that were resistant to ticarcillin and showed reduced susceptibility to carbapenems and cefepime. The bla(OXA-198) gene was harbored by a class 1 integron carried by a ca. 46-kb nontypeable plasmid. This study describes a novel class D β-lactamase involved in carbapenem resistance in P. aeruginosa.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
El Garch, Farid; CHU Mont-Godinne > Laboratoire de bactériologie
Bogaerts, Pierre; CHU Mont-Godinne > Laboratoire de bactériologie
Bebrone, Carine ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Galleni, Moreno ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Glupczynski, Youri; CHU Mont-Godinne > Laboratoire de bactériologie
Language :
English
Title :
OXA-198, an acquired carbapenem-hydrolyzing class D beta-lactamase from Pseudomonas aeruginosa.
Publication date :
October 2011
Journal title :
Antimicrobial Agents and Chemotherapy
ISSN :
0066-4804
eISSN :
1098-6596
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
FP7 - 241742 - TEMPOTEST-QC - An Integrated Tool-Kit for the Clinical Evaluation of Microbial Detection and Antibiotic Susceptibility Point-of-Care Testing Technologies
Funders :
BAPCOC - Belgian Antibiotic Policy Coordination Committee [BE] FPS Health Federal Public Service Health, Food Chain Safety and Environment [BE] EU - European Union [BE] Région wallonne [BE]
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