Reference : Deep sequencing reveals abundant non-canonical retroviral microRNAs in B-cell leukemi...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/138327
Deep sequencing reveals abundant non-canonical retroviral microRNAs in B-cell leukemia/lymphoma
English
Rosewick, Nicolas [Université Libre de Bruxelles (ULB) > Institut Jules Bordet > Laboratory of Experimental Hematology > >]
Momont, Mélanie mailto [Université de Liège - ULiège > > > GIGA - Membres]
Durkin, Keith mailto [Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale >]
Takeda, Haruko mailto [Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale >]
Caiment, Florian [> >]
Cleuter, Yvette [> >]
Vernin, Céline [> >]
Mortreux, Franck [> >]
Wattel, Eric [> >]
Burny, Arsène [> >]
Georges, Michel mailto [Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale >]
Van den Broeke, Anne [Université Libre de Bruxelles (ULB) > Institut Jules Bordet > Laboratory of Experimental Hematology > >]
23-Jan-2013
Proceedings of the National Academy of Sciences of the United States of America
National Academy of Sciences
Yes (verified by ORBi)
International
0027-8424
1091-6490
Washington
DC
[en] non-coding RNA ; oncogenesis ; retrovirus silencing
[en] Viral tumor models have significantly contributed to our understanding of oncogenic mechanisms.
How transforming delta-retroviruses induce malignancy however remains poorly understood,
especially as viral mRNA/protein are tightly silenced in tumors. Here, using deep sequencing of
broad windows of small RNA sizes in the Bovine Leukemia Virus ovine model of
leukemia/lymphoma, we provide in vivo evidence of the production of non-canonical Pol IIItranscribed viral microRNAs in leukemic B-cells in the complete absence of Pol II 5’ LTR-driven
transcriptional activity. Processed from a cluster of five independent self-sufficient transcriptional
units located in a proviral region dispensable for in vivo infectivity, BLV microRNAs represent ~
40 % of all microRNAs in both experimental and natural malignancy. They are subject to strong
purifying selection and associate with Argonautes, consistent with a critical function in silencing of
important cellular and/or viral targets. BLV microRNAs are strongly expressed in preleukemic and
malignant cells in which structural and regulatory gene expression is repressed, suggesting a key
role in tumor onset and progression. Understanding how Pol III-dependent microRNAs subvert
cellular and viral pathways will contribute in deciphering the intricate perturbations that underlie
malignant transformation.
Researchers ; Professionals
http://hdl.handle.net/2268/138327
http://reflexions.ulg.ac.be/virusleucemie

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