Keywords :
Amino Acid Sequence; Binding Sites; Chromosomes, Bacterial/enzymology; Citrobacter/enzymology; Isoenzymes/antagonists & inhibitors; Penicillanic Acid/pharmacology; Peptide Fragments/analysis; beta-Lactamases/antagonists & inhibitors
Abstract :
[en] Both forms of the chromosome-encoded beta-lactamase of Citrobacter diversus react with beta-iodopenicillanate at a rate characteristic of class A beta-lactamases. The active site of form I was labelled with the same reagent. The sequence of the peptide obtained after trypsin hydrolysis is identical with that of a peptide obtained in a similar manner from the chromosome-encoded beta-lactamase of Klebsiella pneumoniae.
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