Establishment of an interactomic map of the Ets factors family: Towards a better understanding of their roles in oncogenic processes

Ets transcription factors play key roles in several cancers such as leukemia, prostate cancer and Ewing’s sarcoma.
They regulate the expression of genes controlling biological processes such as cellular proliferation, differentiation,
apoptosis, metastasis, and transformation. This family is characterized by a highly conserved DNA-binding domain (ETS
domain) and is classified into subfamilies according to sequence homology between the members.
Using a high-throughput yeast two-hybrid (Y2H) method, we tested the interaction of the major splicing variants of the
28 human Ets factors and their functional domains of interest against the last available version of the human
ORFeome (hORFeome v5.1). This screen has identified more than 200 new partners of Ets proteins.
Further validation of these new interactions together with previously described interactions will enable a global
evaluation of the regulation, and normal and cancerous roles of Ets factors.