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Potentiality of using microbial biosensors for the detection of substrate heterogeneities and the assessment of microbial viability in industrial bioreactors: a complete set of experiments in chemostat and scale-down reactors, and elaboration of a mini scale-down platform
Brognaux, Alison; Neubauer, Peter; Twizere, Jean-Claude et al.
2012In Communications in Agricultural and Applied Biological Sciences, 77 (1), p. 3-7
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Keywords :
biosensors; scale-up; stress gene
Abstract :
[en] Substrate limitation responsive biosensors have been used in order to detect spatial substrate heterogeneities, , inside industrial bioreactors (whole-cell biosensor). Three green fluorescent protein (GFP) transcriptional reporters have been chosen in E.coli, i.e. uspA::gfp, csiE::gfp and yciG::gfp. The promoter uspA is induced in response to a variety of stresses whereas the two other promoters, csiE and yciG, are supposed to be more specific in front of a substrate limitation. The responsiveness of these biosensors has been assessed in chemostat reactor. Secondly, the same biosensors have been tested in well-mixed laboratory reactors and in scale-down reactors able to reproduce industrial conditions. Finally, a mini scale-down platform has been proposed as a high throughput tool to investigate rapidly the usefulness of a given microbial biosensor. Local heterogeneities in mini-bioreactor have caused a decrease of GFP expression, as in scale-down reactor. The presence of GFP in supernatants was noticed and this leakage seems to be correlated with the membrane permeability.
Disciplines :
Biotechnology
Author, co-author :
Brognaux, Alison ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Neubauer, Peter
Twizere, Jean-Claude  ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Delvigne, Frank  ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
Potentiality of using microbial biosensors for the detection of substrate heterogeneities and the assessment of microbial viability in industrial bioreactors: a complete set of experiments in chemostat and scale-down reactors, and elaboration of a mini scale-down platform
Publication date :
2012
Event name :
17th Symposium on Applied and Biological Sciences
Event organizer :
Universiteit Gent. Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen
Event place :
Leuven, Belgium
Event date :
10 février 2012
By request :
Yes
Journal title :
Communications in Agricultural and Applied Biological Sciences
ISSN :
1379-1176
Publisher :
Universiteit Gent. Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, Gent, Belgium
Volume :
77
Issue :
1
Pages :
3-7
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 17 February 2012

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