Genetic determination and localization of multiple bacteriocins produced by Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431

[en] Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 from artisanalproduced Peruvian cheeses showed the presence of 4 putative bacteriocin genes: enterocin A, enterocin B, enterocin P, and mundticin KS. The multiple bacteriocin producer E. faecium CWBI-B1430 presented 1 plasmid of 34.6 kb, whereas E. mundtii CWBI-B1431 contained 1 plasmid of 11.0 kb. The structural gene responsible for mundticin KS production was located on 5.6 and 3.1 kb HindIII plasmid fragments. The reverse transcription-PCR analysis showed the expression of the bacteriocin genes enterocin A, enterocin B, and mundticin KS in E. faecium CWBI-B1430 and the bacteriocin genes enterocin P and mundticin KS in E. mundtii CWBI-B1431. To our knowledge, this is the first report of the expression of mundticin KS in E. faecium and enterocin P in E. mundtii.

Disciplines :

Microbiology

Author, co-author :

Aguilar Galvez, Ana Consuelo ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.

Dubois Dauphin, Robin ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries

Campos Gutierrez, David Carlos; Universidad Nacional Agraria La Molina > Instituto de Biotecnología

Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles

Language :

English

Title :

Genetic determination and localization of multiple bacteriocins produced by Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431

Publication date :

2011

Journal title :

Food Science and Biotechnology

ISSN :

1226-7708

Publisher :

Korean Society of Food Science and Technology, South Korea

Volume :

Issue :

Pages :

289-296

Peer reviewed :

Peer reviewed

Available on ORBi :

since 07 February 2012

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Bibliography

Franzetti, L., Pompei, M., Scarpellini, M., Galli, A., Phenotypic and genotypic characterization of Enterococcus spp. of different origins (2004) Curr. Microbiol., 49, pp. 255-260
Giraffa, G., Functionality of enterococci in dairy products (2003) Int. J. Food Microbiol., 88, pp. 215-222
Lim, S., Park, M., Chang, D., Characterization of bacteriocin produced by Enterococcus faecium MJ-14 isolated from meju (2005) Food Sci. Biotechnol., 14, pp. 49-57
Ben Belgacem, Z., Ferchichi, M., Prévost, H., Dousset, X., Manai, M., Screening for anti-listerial bacteriocin-producing lactic acid bacteria from 'Gueddid' a traditionally Tunisian fermented meat (2007) Meat Sci., 78, pp. 513-521
Foulquié, M., Sarantinopoulos, P., Tsakalidou, E., de Vuyst, L., The role and application of enterococci in food and health (2006) Int. J. Food Microbiol., 106, pp. 1-24
Franz, C.H., van Belkum, M., Holzapfel, W., Abriouel, H., Gálvez, A., Diversity of enterococcal bacteriocins and their grouping in a new classification scheme (2007) FEMS Microbiol. Rev., 31, pp. 293-310
Ghrairi, T., Frere, J., Berjeaud, J., Manai, M., Purification and characterisation of bacteriocins produced by Enterococcus faecium from Tunisian rigouta cheese (2008) Food Control, 19, pp. 162-169
Aguilar-Galvez, A., Dubois-Dauphin, R., Ghalfi, H., Campos, D., Thonart, P., Description of two Enterococcus strains isolated from traditional Peruvian artisanal-produced cheeses with a bacteriocinlike inhibitory (2009) Biotechnol. Agron. Soc., 13, pp. 349-356
Heilig, J., Elbing, K., Brent, R., Large-scale preparation of plasmid DNA (2002) Current Protocols in Molecular Biology, pp. 9-10. , In, Ausuhel F, Brent R, Kingston R, Moore D, Seidman J, Smith J, Struhl K (eds). John Wiley and Sons, New York, NY, USA
Park, S., Itoh, K., Fujisawa, T., Characteristics and identification of enterocins produced by Enterococcus faecium JCM 5804T (2003) J. Appl. Microbiol., 95, pp. 294-300
Foulquié, M., Callewaert, R., Devreese, B., van Beeumen, J., de Vuyst, L., Isolation and biochemical characterization of enterocins produced by enterococci from different sources (2003) J. Appl. Microbiol., 94, pp. 214-229
Cintas, L., Casaus, P., Herranz, C., Sigve, L., Holo, H., Hernández, P., Nes, I., Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q (2000) J. Bacteriol., 182, pp. 6806-6814
Gutierrez, J., Criado, R., Citti, R., Martín, M., Herranz, C., Nes, I., Cintas, L., Hernández, P., Cloning, production, and functional expression of enterocin P, a sec-dependent bacteriocin produced by Enterococcus faecium P13, in Escherichia coli (2005) Int. J. Food Microbiol., 103, pp. 239-250
Saavedra, L., Minahk, C., Ruiz, A., Sesma, F., Enhancement of the enterocin CRL35 activity by a synthetic peptide derived from the NH2-terminal sequence (2004) Antimicrob. Agents Ch., 48, pp. 2778-2781
Zendo, T., Eungruttanagorn, N., Fujioka, S., Tashiro, Y., Nomura, K., Sera, Y., Kobayashi, G., Sonomoto, K., Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean (2005) J. Appl. Microbiol., 99, pp. 1181-1190
Kawamoto, S., Shima, J., Sato, R., Eguchi, T., Ohmomo, S., Shibato, J., Horikoshi, N., Sameshima, T., Biochemical and genetic characterization of mundticin KS, an antilisterial peptide produced by Enterococcus mundtii NFRI 7393 (2002) Appl. Environ. Microb., 68, pp. 3830-3840
Aymerich, T., Holo, H., Håvarstein, L., Hugas, M., Garriga, M., Nes, I., Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins (1996) Appl. Environ. Microb., 62, pp. 1676-1682
Cintas, L., Casaus, P., Håvarstein, L., Hernández, P., Nes, I., Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum (1997) Appl. Environ. Microb., 63, pp. 4321-4330
Casaus, P., Nilsen, T., Cintas, L., Nes, I., Hernández, P., Holo, H., Enterocin B, new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A (1997) Microbiology, 143, pp. 2287-2294
Cintas, L., Casaus, P., Fernández, M., Hernández, P., Comparative antimicrobial activity of enterocin L50, pediocin PA-1, nisin A, and lactocin S against spoilage and foodborne pathogenic bacteria (1998) Food Microbiol., 15, pp. 289-298
O'Keeffe, T., Hill, C., Ross, P., Characterization and heterologous expression of the genes encoding enterocin A production, immunity, and regulation in Enterococcus faecium DPC1146 (1999) Appl. Environ. Microb., 65, pp. 1506-1515
du Toit, D., Franz, C., Dicks, L., Holzapfel, W., Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faeces (2000) J. Appl. Microbiol., 88, pp. 482-494
Ennahar, S., Asou, Y., Zendo, T., Sonomoto, K., Ishizaki, A., Biochemical and genetic evidence for production of enterocins A and B by Enterococcus faecium WHE 81 (2001) Int. J. Food Microbiol., 70, pp. 291-301
Herranz, C., Casaus, P., Mukhopadhyay, S., Martínez, J., Rodríguez, J., Nes, I., Hernández, P., Cintas, L., Enterococcus faecium P21: A strain occurring naturally in dry-fermented sausages producing the class II bacteriocins enterocin A and enterocin B (2001) Food Microbiol., 18, pp. 115-131
Mareková, M., Lauková, A., de Vuyst, L., Skaugen, M., Nes, I., Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13 (2003) J. Appl. Microbiol., 94, pp. 523-530
Cocolin, L., Foschino, R., Comi, G., Grazia, M., Description of the bacteriocins produced by two strains of Enterococcus faecium isolated from Italian goat milk (2007) Food Microbiol., 24, pp. 752-758
Lauková, A., Czikková, S., Vasilková, Z., Juriš, P., Mareková, M., Occurrence of bacteriocin production among environmental enterococci (1998) Lett. Appl. Microbiol., 27, pp. 178-182
Bennik, M., Vanloo, B., Brasseur, R., Gorris, L., Smid, E., A novel bacteriocin with a YGNGV motif from vegetable-associated Enterococcus mundtii: Full characterization and interaction with target organisms (1998) Biochim. Biophys. Acta, 1373, pp. 47-58
Campos, C., Rodríguez, O., Calo-Mata, P., Prado, M., Barros-Velázquez, J., Preliminary characterization of bacteriocins from Lactococcus lactis, Enterococcus faecium, and Enterococcus mundtii strains isolated from turbot (Psetta maxima) (2006) Food Res. Int., 39, pp. 356-364
Ferreira, A., Canal, N., Morales, D., Bopp, D., Corção, G., Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces (2007) Braz. Arch. Biol. Techn., 50, pp. 249-258
Criado, R., Gutiérrez, J., Martín, M., Herranz, C., Hernández, C., Cintas, L., Immunochemical characterization of temperature-regulated production of enterocin L50 (EntL50A and EntL50B), enterocin P, and enterocin Q by Enterococcus faecium L50 (2006) Appl. Environ. Microb., 72, pp. 7634-7643
Abriouel, H., Ben Omar, N., Lucas, R., Martínez-Cañamero, M., Gálvez, A., Bacteriocin production, plasmid content, and plasmid location of enterocin P structural gene in enterococci isolated from food sources (2006) Lett. Appl. Microbiol., 42, pp. 331-337
Aymerich, T., Artigas, M., Garriga, M., Monfort, J., Hugas, M., Effect of sausage ingredients and additives on the production of enterocins A and B by Enterococcus faecium CTC492. Optimization of in vitro production and anti-listerial effect in dry fermented sausages (2000) J. Appl. Microbiol., 88, pp. 686-694
Drider, D., Fimland, G., Héchard, Y., McMullen, L., Prévost, H., The continuing story of class IIa bacteriocins (2006) Microbiol. Mol. Biol. R., 70, pp. 564-582
Franz, C., Worobo, R., Quadri, L., Schillinger, U., Holzapfel, W., Vederas, J., Stiles, M., Atypical genetic locus associated with constitutive production of enterocin B by Enterococcus faecium BFE 900 (1999) Appl. Environ. Microb., 65, pp. 2170-2178
Quadri, L., Yan, L., Stiles, M., Vederas, J., Effect of amino acid substitutions on the activity of carnobacteriocin B2 (1997) J. Biol. Chem., 272, pp. 3384-3388
van Belkum, M., Stiles, M., Nonlantibiotic antibacterial peptides from lactic acid bacteria (2000) Nat. Prod. Rep., 17, pp. 323-365