Qualitative and quantitative evaluation of bisindole usambarane alkaloids in Strychnos usambarensis roots by high performance liquid chromatography diode-array

Frederich, Michel; Tits, Monique; Angenot, Luc

doi:10.1002/(SICI)1099-1565(199803/04)9:2<63::AID-PCA388>3.0.CO;2-9

Article (Scientific journals)

Qualitative and quantitative evaluation of bisindole usambarane alkaloids in Strychnos usambarensis roots by high performance liquid chromatography diode-array

Frederich, Michel; Tits, Monique; Angenot, Luc

1998 • In Phytochemical Analysis, 9 (2), p. 63-66

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https://hdl.handle.net/2268/10832

DOI
10.1002/(SICI)1099-1565(199803/04)9:2<63::AID-PCA388>3.0.CO;2-9

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Keywords :

diode-array detector; Strychnos usambarensis; bisindole usambarane alkaloids; high performance liquid chromatography

Abstract :

[en] A high performance liquid chromatographic method for the simultaneous determination of bisindole usambarane alkaloids from the roots of Strychnos usambarensis using a diode-array detector is proposed, The system, consisting of a RP-8 Select B column and a linear gradient elution with acetonitrile and acetate buffer allowed the separation of all the alkaloids in 20 min, Usambarensine was found to be the major tertiary alkaloid present in the roots, In order to demonstrate the possibility of quantifying the bisindole alkaloids in crude roots extracts, the quantitative evaluation of 3',4'-dihydrousambarensine, usambarensine and N-b-methylusambarensine was carried out. (C) 1998 John Wiley & Sons, Ltd.

Disciplines :

Biochemistry, biophysics & molecular biology
Chemistry
Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie

Tits, Monique ; Université de Liège - ULiège > Département de pharmacie > Phytochimie et phytothérapie

Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie

Language :

English

Title :

Qualitative and quantitative evaluation of bisindole usambarane alkaloids in Strychnos usambarensis roots by high performance liquid chromatography diode-array

Publication date :

1998

Journal title :

Phytochemical Analysis

ISSN :

0958-0344

eISSN :

1099-1565

Publisher :

John Wiley & Sons Ltd., West Sussex, United Kingdom

Volume :

Issue :

Pages :

63-66

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 09 April 2009

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Bibliography

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