Poster (Scientific congresses and symposiums)
Evaluation of the contamination of North Sea Porpoises (Phocoena phocoena) by endocrine disrupting chemicals using CG-MS and in vitro assays
Didimo Imazaki, Pedro Henrique; Brose, François; Remacle, Anne-Sophie et al.
20095th Copenhagen Workshop on Endocrine Disrupters
 

Files


Full Text
imazaki_abstract_cow_2009_ver_2_0.pdf
Author preprint (15.99 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
endocrine disrupters; harbour porpoise; North Sea
Abstract :
[en] During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be very slowly degraded, decreasing the environmental quality and causing ecological risks. Marine mammals inhabiting polluted environments accumulate high quantities of these chemicals, and are good indicators of marine pollution Thirteen major organochloride pollutants (known from literature to contaminate north sea porpoise) were chosen in this study – o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. The thirteen chemicals were tested individually and in mixtures by the use of report gene expression assays. The MCF7-ERE cells used in the assays were originated from MCF7 human mammary tumor cells transfected with the ERE-luciferase reporter system. Preliminary results suggest that o,p’-DDD; p,p’-DDD; o,p’-DDT; HCB; β-HCH; δ-HCH; PCB 138 and PCB 180 are able to stimulate the luciferase expression of MCF7-ERE cells when they are present individually or in mixtures. No synergism was observed within mixtures. When MCF7-ERE cells were exposed to the chemicals and 17β-oestradiol simultaneously, p,p’-DDE; α-HCH; PCB 138 and PCB 180 could raise the luciferase expression in comparison to the exposition of 17β-oestradiol alone; on the other hand, o,p’-DDD; p,p’-DDT; HCB; β-HCH; γ-HCH and δ-HCH seem to inhibit the luciferase expression at low doses. In the next step, the pollutants will be searched and quantified in the blubber tissue of porpoises by use of gas chromatography-mass spectrometry. The blubber tissue samples will also have their endocrine disrupting effects characterized. This work is ongoing and It is expected a relevant level of these compounds to be found in the samples. Conversely, their endocrine disrupting effects and how they act in agonist, antagonist ways are subject that still needs to be studied.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Didimo Imazaki, Pedro Henrique ;  Université de Liège - ULiège > Doct. sc. vété. (Bologne)
Brose, François ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires
Remacle, Anne-Sophie ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Van Der Heiden, Edwige ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Scippo, Marie-Louise  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Language :
English
Title :
Evaluation of the contamination of North Sea Porpoises (Phocoena phocoena) by endocrine disrupting chemicals using CG-MS and in vitro assays
Publication date :
2009
Event name :
5th Copenhagen Workshop on Endocrine Disrupters
Event place :
Copenhague, Denmark
Event date :
du 20 au 22 mai 2009
Audience :
International
Additional URL :
Available on ORBi :
since 12 December 2011

Statistics


Number of views
75 (6 by ULiège)
Number of downloads
62 (6 by ULiège)

Bibliography


Similar publications



Contact ORBi