Transforming growth factor-beta1-mediated Slug and Snail transcription factor up-regulation reduces the density of Langerhans cells in epithelial metaplasia by affecting E-cadherin expression
[en] Epithelial metaplasia (EpM) is an acquired tissue abnormality resulting from the transformation of epithelium into another tissue with a different structure and function. This adaptative process is associated with an increased frequency of (pre)cancerous lesions. We propose that EpM is involved in cancer development by altering the expression of adhesion molecules important for cell-mediated antitumor immunity. Langerhans cells (LCs) are intraepithelial dendritic cells that initiate immune responses against viral or tumor antigens on both skin and mucosal surfaces. In the present study, we showed by immunohistology that the density of CD1a LCs is reduced in EpM of the uterine cervix compared with native squamous epithelium and that the low number of LCs observed in EpM correlates with the down-regulation of cell-surface E-cadherin. We also demonstrated that transforming growth factor- 1 is not only overexpressed in metaplastic tissues but also reduces E-cadherin expression in keratinocytes in vitro by inducing the promoter activity of Slug and Snail transcription factors. Finally, we showed that in vitro-generated LCs adhere poorly to keratinocytes transfected with either Slug or Snail DNA. These data suggest that transforming growth factor- 1 indirectly reduces antigenpresenting cell density in EpM by affecting E-cadherin expression, which might explain the increased susceptibility of abnormal tissue differentiation to the development of cancer by the establishment of local immunodeficiency responsible for EpM tumorigenesis.
Disciplines :
Oncology Immunology & infectious disease
Author, co-author :
Herfs, Michael ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Hubert, Pascale ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Kholod, Natalia
Caberg, Jean-Hubert ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Gilles, Christine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Berx, Geert
Savagner, Pierre
Boniver, Jacques ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Language :
English
Title :
Transforming growth factor-beta1-mediated Slug and Snail transcription factor up-regulation reduces the density of Langerhans cells in epithelial metaplasia by affecting E-cadherin expression
Publication date :
May 2008
Journal title :
American Journal of Pathology
ISSN :
0002-9440
eISSN :
1525-2191
Publisher :
American Society for Investigative Pathology, Bethesda, United States - Maryland
JM Slack Metaplasia and transdifferentiation: from pure biology to the clinic Nat Rev Mol Cell Biol 8 2007 369 378
D Tosh JM Slack How cells change their phenotype Nat Rev Mol Cell Biol 3 2002 187 194
DA Elson RR Riley A Lacey G Thordarson FJ Talamantes JM Arbeit Sensitivity of the cervical transformation zone to estrogen-induced squamous carcinogenesis Cancer Res 60 2000 1267 1275
JM Quinlan BJ Colleypriest M Farrant D Tosh Epithelial metaplasia and the development of cancer Biochim Biophys Acta 1776 2007 10 21
E Burghardt AG Ostor Site and origin of squamous cervical cancer: a histomorphologic study Obstet Gynecol 62 1983 117 127
P Delvenne P Hubert N Jacobs Epithelial metaplasia: an inadequate environment for antitumour immunity? Trends Immunol 25 2004 169 173
H Uchi H Terao T Koga M Furue Cytokines and chemokines in the epidermis J Dermatol Sci 24 Suppl 1 2000 S29 S38
M Sikorski T Bieda M Bobek H Zrubek Dynamics of cervical Langerhans cell counts in the course of HPV-positive CIN treatment with the use of human recombinant interferon gamma Eur J Gynaecol Oncol 26 2005 294 298
W Al-Saleh SL Giannini N Jacobs M Moutschen J Doyen J Boniver P Delvenne Correlation of T-helper secretory differentiation and types of antigen-presenting cells in squamous intraepithelial lesions of the uterine cervix J Pathol 184 1998 283 290
SL Giannini P Hubert J Doyen J Boniver P Delvenne Influence of the mucosal epithelium microenvironment on Langerhans cells: implications for the development of squamous intraepithelial lesions of the cervix Int J Cancer 97 2002 654 659
A Tang M Amagai LG Granger JR Stanley MC Udey Adhesion of epidermal Langerhans cells to keratinocytes mediated by E-cadherin Nature 361 1993 82 85
KL Cepek SK Shaw CM Parker GJ Russell JS Morrow DL Rimm MB Brenner Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin Nature 372 1994 190 193
T Bailey L Biddlestone N Shepherd H Barr P Warner J Jankowski Altered cadherin and catenin complexes in the Barrett's esophagus-dysplasia-adenocarcinoma sequence: correlation with disease progression and dedifferentiation Am J Pathol 152 1998 135 144
AO Chan BC Wong HY Lan SL Loke WK Chan WM Hui YH Yuen I Ng L Hou WM Wong MF Yuen JM Luk SK Lam Deregulation of E-cadherin-catenin complex in precancerous lesions of gastric adenocarcinoma J Gastroenterol Hepatol 18 2003 534 539
Y Kato T Hirano K Yoshida K Yashima S Akimoto K Tsuji T Ohira M Tsuboi N Ikeda Y Ebihara H Kato Frequent loss of E-cadherin and/or catenins in intrabronchial lesions during carcinogenesis of the bronchial epithelium Lung Cancer 48 2005 323 330
JR Graff JG Herman RG Lapidus H Chopra R Xu DF Jarrard WB Isaacs PM Pitha NE Davidson SB Baylin E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas Cancer Res 55 1995 5195 5199
K Yoshiura Y Kanai A Ochiai Y Shimoyama T Sugimura S Hirohashi Silencing of the E-cadherin invasion-suppressor gene by CpG methylation in human carcinomas Proc Natl Acad Sci USA 92 1995 7416 7419
E Batlle E Sancho C Franci D Dominguez M Monfar J Baulida HA Garcia De The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells Nat Cell Biol 2 2000 84 89
V Bolós H Peinado MA Perez-Moreno MF Fraga M Esteller A Cano The transcription factor Slug represses E-cadherin expression and induces epithelial to mesenchymal transitions: a comparison with Snail and E47 repressors J Cell Sci 116 2003 499 511
A Cano MA Perez-Moreno I Rodrigo A Locascio MJ Blanco MG del Barrio F Portillo MA Nieto The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression Nat Cell Biol 2 2000 76 83
KM Hajra DY Chen ER Fearon The SLUG zinc-finger protein represses E-cadherin in breast cancer Cancer Res 62 2002 1613 1618
A Miyoshi Y Kitajima K Sumi K Sato A Hagiwara Y Koga K Miyazaki Snail and SIP1 increase cancer invasion by upregulating MMP family in hepatocellular carcinoma cells Br J Cancer 90 2004 1265 1273
A Miyoshi Y Kitajima S Kido T Shimonishi S Matsuyama K Kitahara K Miyazaki Snail accelerates cancer invasion by upregulating MMP expression and is associated with poor prognosis of hepatocellular carcinoma Br J Cancer 92 2005 252 258
K Sugimachi S Tanaka T Kameyama K Taguchi S Aishima M Shimada K Sugimachi M Tsuneyoshi Transcriptional repressor snail and progression of human hepatocellular carcinoma Clin Cancer Res 9 2003 2657 2664
RG Hardy C Vicente-Duenas I Gonzalez-Herrero C Anderson T Flores S Hughes C Tselepis JA Ross I Sanchez-Garcia Snail family transcription factors are implicated in thyroid carcinogenesis Am J Pathol 171 2007 1037 1046
J Choi SY Park CK Joo Transforming growth factor-beta1 represses E-cadherin production via slug expression in lens epithelial cells Invest Ophthalmol Vis Sci 48 2007 2708 2718
D Medici ED Hay DA Goodenough Cooperation between snail and LEF-1 transcription factors is essential for TGF-beta1-induced epithelial-mesenchymal transition Mol Biol Cell 17 2006 1871 1879
H Peinado M Quintanilla A Cano Transforming growth factor beta-1 induces snail transcription factor in epithelial cell lines: mechanisms for epithelial mesenchymal transitions J Biol Chem 278 2003 21113 21123
G Monteleone F Pallone TT MacDonald Smad7 in TGF-beta-mediated negative regulation of gut inflammation Trends Immunol 25 2004 513 517
PM Siegel J Massague Cytostatic and apoptotic actions of TGF-beta in homeostasis and cancer Nat Rev Cancer 3 2003 807 821
P Delvenne MA Fontaine C Delvenne A Nikkels J Boniver Detection of human papillomaviruses in paraffin-embedded biopsies of cervical intraepithelial lesions: analysis by immunohistochemistry, in situ hybridization, and the polymerase chain reaction Mod Pathol 7 1994 113 119
W Qu G Jiang Y Cruz CJ Chang GY Ho RS Klein RD Burk PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems J Clin Microbiol 35 1997 1304 1310
PJ Snijders AJ van den Brule HF Schrijnemakers G Snow CJ Meijer JM Walboomers The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes J Gen Virol 71 1990 173 181
J Zavadil L Cermak N Soto-Nieves EP Bottinger Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition EMBO J 23 2004 1155 1165
C Detry D Waltregny P Quatresooz M Chaplet W Kedzia V Castronovo P Delvenne A Bellahcene Detection of bone sialoprotein in human (pre)neoplastic lesions of the uterine cervix Calcif Tissue Int 73 2003 9 14
P Hubert JH Caberg C Gilles L Bousarghin E Franzen-Detrooz J Boniver P Delvenne E-cadherin-dependent adhesion of dendritic and Langerhans cells to keratinocytes is defective in cervical human papillomavirus-associated (pre)neoplastic lesions J Pathol 206 2005 346 355
P Delvenne W Al-Saleh C Gilles A Thiry J Boniver Inhibition of growth of normal and human papillomavirus-transformed keratinocytes in monolayer and organotypic cultures by interferon-gamma and tumor necrosis factor-alpha Am J Pathol 146 1995 589 598
M Jordà D Olmeda A Vinyals E Valero E Cubillo A Llorens A Cano A Fabra Upregulation of MMP-9 in MDCK epithelial cell line in response to expression of the Snail transcription factor J Cell Sci 118 2005 3371 3385
MK Tripathi S Misra SV Khedkar N Hamilton C Irvin-Wilson C Sharan L Sealy G Chaudhuri Regulation of BRCA2 gene expression by the SLUG repressor protein in human breast cells J Biol Chem 280 2005 17163 17171
P Hubert L Bousarghin R Greimers E Franzen-Detrooz J Boniver P Delvenne Production of large numbers of Langerhans' cells with intraepithelial migration ability in vitro Exp Dermatol 14 2005 469 477
MJ Cossentino RK Wong Barrett's esophagus and risk of esophageal adenocarcinoma Semin Gastrointest Dis 14 2003 128 135
JL Whiting A Sigurdsson DC Rowlands MT Hallissey JW Fielding The long term results of endoscopic surveillance of premalignant gastric lesions Gut 50 2002 378 381
CP Wild LJ Hardie Reflux. Barrett's oesophagus and adenocarcinoma: burning questions Nat Rev Cancer 3 2003 676 684
SL Giannini W Al-Saleh H Piron N Jacobs J Doyen J Boniver P Delvenne Cytokine expression in squamous intraepithelial lesions of the uterine cervix: implications for the generation of local immunosuppression Clin Exp Immunol 113 1998 183 189
C Tselepis I Perry C Dawson R Hardy SJ Darnton C McConkey RC Stuart N Wright R Harrison JA Jankowski Tumour necrosis factor-alpha in Barrett's oesophagus: a potential novel mechanism of action Oncogene 21 2002 6071 6081
S Swami S Kumble G Triadafilopoulos E-cadherin expression in gastroesophageal reflux disease. Barrett's esophagus, and esophageal adenocarcinoma: an immunohistochemical and immunoblot study Am J Gastroenterol 90 1995 1808 1813
RS Barnetson A Satchell L Zhuang HB Slade GM Halliday Imiquimod induced regression of clinically diagnosed superficial basal cell carcinoma is associated with early infiltration by CD4 T cells and dendritic cells Clin Exp Dermatol 29 2004 639 643
N Inoshima Y Nakanishi T Minami M Izumi K Takayama I Yoshino N Hara The influence of dendritic cell infiltration and vascular endothelial growth factor expression on the prognosis of non-small cell lung cancer Clin Cancer Res 8 2002 3480 3486
TE Reichert C Scheuer R Day W Wagner TL Whiteside The number of intratumoral dendritic cells and zeta-chain expression in T cells as prognostic and survival biomarkers in patients with oral carcinoma Cancer 91 2001 2136 2147
K Matthews CM Leong L Baxter E Inglis K Yun BT Backstrom J Doorbar M Hibma Depletion of Langerhans cells in human papillomavirus type 16-infected skin is associated with E6-mediated down regulation of E-cadherin J Virol 77 2003 8378 8385
G Böhmer AJ van den Brule O Brummer CL Meijer KU Petry No confirmed case of human papillomavirus DNA-negative cervical intraepithelial neoplasia grade 3 or invasive primary cancer of the uterine cervix among 511 patients Am J Obstet Gynecol 189 2003 118 120
FX Bosch N Munoz The viral etiology of cervical cancer Virus Res 89 2002 183 190
JM Walboomers MV Jacobs MM Manos FX Bosch JA Kummer KV Shah PJ Snijders J Peto CJ Meijer N Munoz Human papillomavirus is a necessary cause of invasive cervical cancer worldwide J Pathol 189 1999 12 19
CA Eads RV Lord K Wickramasinghe TI Long SK Kurumboor L Bernstein JH Peters SR DeMeester TR Demeester KA Skinner PW Laird Epigenetic patterns in the progression of esophageal adenocarcinoma Cancer Res 61 2001 3410 3418
KF To WK Leung TL Lee J Yu JH Tong MW Chan EK Ng SC Chung JJ Sung Promoter hypermethylation of tumor-related genes in gastric intestinal metaplasia of patients with and without gastric cancer Int J Cancer 102 2002 623 628
B Hotz M Arndt S Dullat S Bhargava HJ Buhr HG Hotz Epithelial to mesenchymal transition: expression of the regulators snail, slug, and twist in pancreatic cancer Clin Cancer Res 13 2007 4769 4776
Y Uchikado S Natsugoe H Okumura T Setoyama M Matsumoto S Ishigami T Aikou Slug expression in the E-cadherin preserved tumors is related to prognosis in patients with esophageal squamous cell carcinoma Clin Cancer Res 11 2005 1174 1180
S Takano F Kanai A Jazag H Ijichi J Yao H Ogawa N Enomoto M Omata A Nakao Smad4 is essential for down-regulation of E-cadherin induced by TGF-beta in pancreatic cancer cell line PANC-1 J Biochem (Tokyo) 141 2007 345 351
S Thuault U Valcourt M Petersen G Manfioletti CH Heldin A Moustakas Transforming growth factor-beta employs HMGA2 to elicit epithelial-mesenchymal transition J Cell Biol 174 2006 175 183
M Dohadwala SC Yang J Luo S Sharma RK Batra M Huang Y Lin L Goodglick K Krysan MC Fishbein L Hong C Lai RB Cameron RM Gemmill HA Drabkin SM Dubinett Cyclooxygenase-2-dependent regulation of E-cadherin: prostaglandin E(2) induces transcriptional repressors ZEB1 and snail in non-small cell lung cancer Cancer Res 66 2006 5338 5345
A Fukunaga H Nagai T Noguchi H Okazawa T Matozaki X Yu CF Lagenaur N Honma M Ichihashi M Kasuga C Nishigori T Horikawa Src homology 2 domain-containing protein tyrosine phosphatase substrate 1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes J Immunol 172 2004 4091 4099
AA Price M Cumberbatch I Kimber A Ager Alpha 6 integrins are required for Langerhans cell migration from the epidermis J Exp Med 186 1997 1725 1735
MJ Staquet Adhesion and migration of epidermal dendritic cells Pathol Biol (Paris) 43 1995 858 862