Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leaves and Other Selected Tropical Green Vegetables Investigated on Lipoperoxidation and Phorbol-12-myristate-13-acetate (PMA) Activated Monocytes
monocytes; lipid peroxidation; reactive oxygen species; green vegetable; antioxidant; polyphenols
Abstract :
[en] Abelmoschus esculentus (Malvaceae), Hibiscus acetosella (Malvaceae), Manihot esculenta Crantz (Euphorbiaceae) and Pteridium aquilinum (Dennstaedtiaceae) leaves are currently consumed as vegetables by migrants from sub-Saharan Africa living in Western Europe and by the people in the origin countries, where these plants are also used in the folk medicine. Manihot leaves are also eaten in Latin America and some Asian countries. This work investigated the capacity of aqueous extracts prepared from those vegetables to inhibit the peroxidation of a linoleic acid emulsion. Short chain, volatile C-compounds as markers of advanced lipid peroxidation were measured by gas chromatography by following the ethylene production. The generation of lipid hydroperoxides, was monitored by spectroscopy using N-N′-dimethyl-p-phenylene-diamine (DMPD). The formation of intermediate peroxyl, and other free radicals, at the initiation of the lipid peroxidation was investigated by electron spin resonance, using α-(4-pyridyl-1-oxide)-N-tert-butylnitrone as spin trap agent. The ability of the extracts to decrease the cellular production of reactive oxygen species (ROS) in “inflammation like” conditions was studied by fluorescence technique using 2′,7′-dichlorofluorescine-diacetate as fluorogenic probe, in a cell model of human monocytes (HL-60 cells) activated with phorbol ester. Overall the extracts displayed efficient concentration-dependent inhibitory effects. Their total polyphenol and flavonoid content was determined by classic colorimetric methods. An HPLC-UV/DAD analysis has clearly identified the presence of some polyphenolic compounds, which explains at least partially the inhibitions observed in our models. The role of these plants in the folk medicine by sub-Saharan peoples as well as in the prevention of oxidative stress and ROS related diseases requires further consideration.
Research Center/Unit :
Faculté de médecine; département de pharmacie, CIRM, laboratoire de pharmacognosie/ Faculté des sciences, centre de l'oxygène, recherche et développement (CORD)
Disciplines :
Food science Veterinary medicine & animal health Biochemistry, biophysics & molecular biology
Deby-Dupont, Ginette; Université de Liège - ULiège > Centre de l'Oxygène, Recherche et Développement, CORD
Tits, Monique ; Université de Liège - ULiège > Département de pharmacie > Phytochimie et phytothérapie
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Language :
English
Title :
Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leaves and Other Selected Tropical Green Vegetables Investigated on Lipoperoxidation and Phorbol-12-myristate-13-acetate (PMA) Activated Monocytes
Alternative titles :
[fr] Activités antioxydantes et antiradicalaires des feuilles de Manihot esculenta (Crantz) et d'autres légumes verts tropicaux sélectionnés, investiguées sur lipoperoxydation et des monocytes activés au phorbol 12-myristate, 13-acétate (PMA)
Publication date :
16 September 2011
Journal title :
Nutrients
ISSN :
2072-6643
Publisher :
Molecular Diversity Preservation International (MDPI), Basel, Switzerland
Volume :
3
Issue :
9
Pages :
818-838
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
SP Partnership Association Lich Biebergemünder Association for South Development International Help Service IPHD Linz
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