Penicillin-binding proteins. Wall peptidoglycan assembly and resistance to penicillin: facts, doubts and hopes

[en] A review with 95 refs. The assembly of the bacterial cell wall peptidoglycan relies upon the availability of a ready-made precursor, the lipid II intermediate. This intermediate is taken up by a multifunctional factory that provides the required enzymic activities for polymer assembly at the exterior of the plasma membrane. Morphogenetic networks regulate the synthesis in a cell-cycle-dependent fashion. As essential components of the cell machinery are targets of Î²-lactam antibiotics, safety devices protect the cells against these toxic agents. Controversy and consensus formation lie at the heart of the scientific research. This review focuses on questions that bacterial cell wall biochemists still strive, with increasing success, to answer. [on SciFinder(R)]
[en] As the protein sequence and structure databases expand, the relationships between proteins, the notion of protein superfamily, and the driving forces of evolution are better understood. Key steps of the synthesis of the bacterial cell wall peptidoglycan are revisited in light of these advances. The reactions through which the D-alanyl-D-alanine depeptide is formed, utilized, and hydrolyzed and the sites of action of the glycopeptide and β-lactam antibiotics illustrate the concept according to which new enzyme functions evolve as a result of tinkering of existing proteins. This occurs by the acquisition of local structural changes, the fusion into mul-timodular polypeptides, and the association into multiprotein complexes.

Research Center/Unit :

CIP - Centre d'Ingénierie des Protéines - ULiège

Disciplines :

Microbiology
Biochemistry, biophysics & molecular biology

Author, co-author :

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines

Charlier, Paulette ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines

Coyette, Jacques ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines

Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Fonzé, Eveline; Université de Liège - ULiège > Centre d'Ingénierie des Protéines

Fraipont, Claudine ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Goffin, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Joris, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes

Nguyen-Distèche, Martine ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Language :

English

Title :

Penicillin-binding proteins. Wall peptidoglycan assembly and resistance to penicillin: facts, doubts and hopes

Publication date :

1997

Journal title :

International Journal of Antimicrobial Agents

ISSN :

0924-8579

eISSN :

1872-7913

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

Issue :

Pages :

45-60

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRSM - Fonds de la Recherche Scientifique Médicale
FRFC - Fonds de la Recherche Fondamentale Collective

Available on ORBi :

since 19 September 2011

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