Transgenic engineering of male-specific muscular hypertrophy.

Animals; Body Weights and Measures; Breeding/methods; DNA-Binding Proteins/genetics; Gene Targeting/methods; Genetic Engineering/methods; Genetic Vectors/genetics; Male; Mice; Mice, Transgenic; Muscle Development/genetics; Muscle, Skeletal/growth & development; Myosin Light Chains/genetics; Myostatin; Nuclear Proteins/genetics; Promoter Regions, Genetic/genetics; Sex-Determining Region Y Protein; Totipotent Stem Cells/metabolism; Transcription Factors/genetics; Transforming Growth Factor beta/genetics/metabolism; Y Chromosome/genetics

Abstract :

[en] Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency-associated myostatin propeptide under control of the myosin light chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass. This experiment demonstrates the feasibility of a more efficient cattle production system combining superior beef production abilities for bulls and dairy abilities for cows.

Disciplines :

Genetics & genetic processes
Veterinary medicine & animal health

Author, co-author :

Pirottin, Dimitri ; Université de Liège - ULiège > Département de productions animales > Génétique factorielle et moléculaire

Grobet, Luc ; Université de Liège - ULiège > Département de morphologie et pathologie > Embryologie

Adamantidis, Antoine ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique

Farnir, Frédéric ; Université de Liège - ULiège > Département de productions animales > Biostatistique, économie, sélection animale

Herens, Christian ; Centre Hospitalier Universitaire de Liège - CHU > Génétique

Daa Schroder, Henrik; Odense Universiteitshospital - OUH > Institute of Pathology

Georges, Michel ; Université de Liège - ULiège > Département de productions animales > Génétique factorielle et moléculaire

Language :

English

Title :

Transgenic engineering of male-specific muscular hypertrophy.

Publication date :

2005

Journal title :

Proceedings of the National Academy of Sciences of the United States of America

ISSN :

0027-8424

eISSN :

1091-6490

Publisher :

National Academy of Sciences, Washington, United States - District of Columbia

Volume :

102

Issue :

Pages :

6413-8

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 June 2011

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