[en] Erythromycin is a mixture of macrolide antibiotics produced by Saccharopolyspora erythreas during fermentation. A new method for the analysis of erythromycin by liquid chromatography has previously been developed. It makes use of an Astec C18 polymeric column. After validation in one laboratory, the method was now validated in an interlaboratory study. Validation studies are commonly used to test the fitness of the analytical method prior to its use for routine quality testing. The data derived in the interlaboratory study can be used to make an uncertainty statement as well. The relationship between validation and uncertainty statement is not clear for many analysts and there is a need to show how the existing data, derived during validation, can be used in practice. Eight laboratories participated in this interlaboratory study. The set-up allowed the determination of the repeatability variance, s(2)r and the between-laboratory variance, s(2)L. Combination of s(2)r and s(2)L results in the reproducibility variance s(2)R. It has been shown how these data can be used in future by a single laboratory that wants to make an uncertainty statement concerning the same analysis.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Bryskier A., Agouridas C., Gasc J.-C. Bryskier A.J., Butzler J.-P., Neu H.C., Tulkens P.M. Macrolides: Chemistry, Pharmacology and Clinical Uses. 1993;Arnette Blackwell, Paris.
Cachet Th., Van den Mooter G., Hauchecorne R., Vinckier C., Hoogmartens J. Int. J. Pharm. 55:1989;59.
Vinckier C., Hauchecorne R., Cachet Th., Van den Mooter G., Hoogmartens J. Int. J. Pharm. 55:1989;67.
Kibwage I.O., Busson R., Janssen G., Hoogmartens J., Vanderhaeghe H., Bracke J. J. Org. Chem. 52:1987;991.
Kirst H.A., Wind J.A., Paschal J.W. J. Org. Chem. 52:1987;4359.
European Pharmacopoeia. 4th ed. 2002;Council of Europe, Strasbourg.
Chepkwony H.K., Dehouck P., Roets E., Hoogmartens J. Chromatographia. 53:2001;159.
Wardrop J., Ficker D., Franklin S., Gorski R.J. J. Pharm. Sci. 89:2000;1097.
Dehouck P., Roets E., Hoogmartens J. Chromatographia. 57:2003;671.
Eurachem/Citac Guide, Quantifying Uncertainty in Analytical Measurement. 2nd ed. 2000.
ICH Harmonised Tripartite Guideline, Text On Validation of Analytical Procedures. 1994;ICH Steering Committee.
ISO/IEC Standard 5725-2, Accuracy (Trueness and Precision) of Measurement Methods and Results. 1994.
Vander Heyden Y., Saevels J., Roets E., Hoogmartens J., Decolin D., Quaglia M.G., Van den Bossche W., Leemans R., Smeets O., Van de Vaart F., Mason B., Taylor G.C., Underberg W., Bult A., Chiap P., Crommen J., De Beer J., Hansen S.H., Massart D.L. J. Chromatogr. A. 830:1999;3.
Dehouck P., Vander Heyden Y., Smeyers-Verbeke J., Massart D.L., Crommen J., Hubert Ph., Marini R.D., Smeets O.S.N.M., Decristoforo G., Van de Wauw W., De Beer J., Quaglia M.G., Stella C., Veuthey J.-L., Estevenon O., Van Schepdael A., Roets E., Hoogmartens J. Anal. Chim. Acta. 481:2003;261.
Boyer K.W., Horwitz W., Albert R. Anal. Chem. 57:1985;454.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.