Large Scale Preparation of Purified Exocellular DD-carboxypeptidase-Transpeptidase of Streptomyces Strain R61

[en] The exocellular DD-carboxypeptidase–transpeptidase that Streptomyces R61 excretes during growth has been produced in large fermentation units of 15 m3 total capacity. The yield from 15,000 liter culture filtrate was 1.080 g purified enzyme (92% purity) with a total recovery of 29% and at least a 2000-fold increased specific activity.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Fossati, P.; Société Rapidase - France

Saint-Ghislain, M.; Société Rapidase - France

Sicard, P. J.; Société Rapidase - France

Frère, Jean-Marie ; Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie

Dusart, Jean; Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie

Klein, Daniel; Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie

Language :

English

Title :

Large Scale Preparation of Purified Exocellular DD-carboxypeptidase-Transpeptidase of Streptomyces Strain R61

Publication date :

April 1978

Journal title :

Biotechnology and Bioengineering

ISSN :

0006-3592

eISSN :

1097-0290

Publisher :

John Wiley & Sons, Inc

Volume :

Pages :

577-587

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

ULiège. Patrimoine - Université de Liège. Patrimoine

Available on ORBi :

since 26 May 2011

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Bibliography

Ghusen J.M. (1977) The bacterial DD‐carboxypeptidase‐transpeptidase enzyme system. A new insight into the mode of action of penicillin. E. R. Squibb Lectures on Chemistry of Microbial Products, W. E. Brown, University of Tokyo Press, Tokyo; .
Ghuysen J.M. J. Gen. Microbiol. 1977, 101:13.
Fr J.M., Leyh‐Bouille M., Ghuysen J.M., Nieto M., Perkins H.R. Meth. Enzymol. , ère; 1976, 45:610.
Lambert A., Neish (1950) RAPID METHOD FOR ESTIMATION OF GLYCEROL IN FERMENTATION SOLUTIONS. Canadian Journal of Research 28 B:83.
Kalckar H.M. J. Biol. Chem. 1947, 167:461.