Article (Scientific journals)
The exchange reaction of peptides R-D-alanyl-D-alanine with D-[14C]alanine to R-D-alanyl-D-[14C]alanine and D-alanine, catalysed by the membranes of Streptococcus faecalis ATCC 9790
Coyette, Jacques; Ghuysen, Jean-Marie; Perkins, Harold R.
1977In European Journal of Biochemistry, 75 (1), p. 225-229
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Keywords :
alanine/*metabolism; carboxypeptidases/*metabolism; cell membrane/*enzymology; detergents/pharmacology; enterococcus faecalis/*enzymology; hydrogen-Ion concentration; kinetics; muramoylpentapeptide carboxypeptidase/*metabolism; oligopeptides/*metabolism; osmolar concentration; structure-activity relationship
Abstract :
[en] Under alkaline conditions, the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC 9790 catalyses exchange reactions in which the X-L-R3-D-Ala moiety of peptides of the type X-L-R3-D-Ala-D-Ala is transferred to simple amino compounds such as D-alanine, glycine and glycyl-glycine. The enzyme system is unable, however, to catalyse complex reactions that would simulate the natural transpeptidation reaction.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Coyette, Jacques;  Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie
Perkins, Harold R.;  University of Liverpool > Department of Microbiology
Language :
English
Title :
The exchange reaction of peptides R-D-alanyl-D-alanine with D-[14C]alanine to R-D-alanyl-D-[14C]alanine and D-alanine, catalysed by the membranes of Streptococcus faecalis ATCC 9790
Publication date :
02 May 1977
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
75
Issue :
1
Pages :
225-229
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRFC - Fonds de la Recherche Fondamentale Collective [BE]
NIH - National Institutes of Health [US-MD]
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